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TESK1 (NM_006285) Human Untagged Clone
CAT＃: SC323413
TESK1 (untagged)-Kinase deficient mutant (K86M) of Human testis-specific kinase 1 (TESK1)
CNY 4,672.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006285, the custom clone sequence may differ by one or more nucleotides
ATGGCCGGGGAACGGCCCCCACTGCGGGGCCCTGGGCCCGGGCCTGGAGAGGTGCCGGGGGAGGGGCCCC CGGGGCCGGGGGGCACGGGCGGAGGCCCGGGCCGGGGCCGCCCCTCCTCCTACCGGGCTCTCCGCAGCGC CGTGTCTAGCCTGGCGCGTGTGGACGATTTTCACTGCGCGGAGAAGATCGGGGCCGGCTTCTTCTCTGAG GTCTACAAGGTTCGGCACCGACAGTCAGGGCAAGTCATGGTGCTGAAGATGAACAAGCTCCCCAGTAACC GGGGCAACACACTACGGGAAGTGCAGCTGATGAACCGGCTCAGGCACCCCAACATCCTAAGGTTCATGGG AGTCTGTGTGCACCAGGGACAGCTGCACGCTCTTACAGAGTATATGAATGGGGGGACATTGGAACAGCTG CTCAGCTCCCCTGAACCCCTGTCCTGGCCGGTCAGGCTCCACCTGGCCCTGGACATTGCCCGAGGCCTGC GGTACCTGCACTCCAAAGGTGTATTTCACCGCGACCTCACATCCAAGAACTGTCTAGTCCGACGGGAAGA TCGAGGCTTCACCGCTGTCGTGGGTGACTTCGGGCTGGCCGAAAAGATTCCTGTGTATAGGGAGGGGGCA AGGAAGGAGCCATTGGCCGTGGTGGGCTCCCCATACTGGATGGCTCCAGAGGTGTTACGGGGTGAGCTGT ATGATGAGAAGGCTGATGTCTTTGCCTTCGGGATTGTCCTCTGTGAGCTCATCGCCCGAGTACCTGCAGA CCCTGACTACCTACCACGCACTGAGGACTTTGGCCTGGATGTGCCTGCTTTCCGAACTCTGGTGGGGGAT GACTGCCCACTGCCTTTCTTGCTCCTGGCCATCCACTGCTGCAACCTGGAACCCAGCACCCGTGCCCCCT TCACCGAAATTACCCAGCACCTGGAATGGATCCTGGAGCAGCTGCCTGAGCCAGCCCCACTCACCAGGAC CGCCCTGACACACAATCAGGGCTCTGTTGCAAGAGGGGGTCCCTCTGCCACGCTTCCCAGGCCAGATCCC CGGCTTTCCCGAAGCCGGTCAGACCTCTTCCTGCCCCCATCACCAGAATCACCCCCCAACTGGGGGGACA ATCTGACTCGAGTCAACCCCTTCTCACTACGGGAAGACCTCAGGGGTGGCAAGATCAAGCTCTTAGACAC ACCCAGCAAGCCAGTCCTGCCTCTTGTGCCACCATCACCATTCCCATCCACTCAGCTGCCCTTGGTGACC ACTCCGGAGACCCTGGTCCAGCCTGGGACACCTGCCCGCCGCTGCCGCTCACTACCCTCATCCCCCGAGC TCCCCCGCCGTATGGAGACAGCACTGCCAGGTCCTGGCCCTCCCGCTGTGGGCCCCTCGGCTGAAGAGAA GATGGAGTGCGAGGGCAGCAGCCCTGAGCCGGAACCTCCAGGGCCAGCGCCCCAGCTGCCTCTGGCTGTG GCCACAGACAACTTCATCAGCACCTGTTCCTCGGCCTCCCAACCCTGGTCCCCTAGATCAGGACCCGTCC TCAATAACAATCCCCCAGCTGTGGTGGTGAACTCCCCACAAGGCTGGGCTGGGGAGCCCTGGAACCGGGC CCAGCATAGCCTGCCCCGGGCGGCAGCCCTGGAGCGGACAGAACCCTCGCCACCCCCTTCAGCTCCCCGG GAGCCCGATGAGGGGCTGCCCTGTCCTGGCTGCTGCCTCGGCCCCTTCAGCTTTGGCTTCCTGTCCATGT GCCCCCGCCCCACACCAGCTGTTGCCCGCTACCGCAACCTGAACTGTGAGGCGGGCAGTCTCCTCTGCCA CCGAGGGCACCACGCCAAGCCACCCACACCCAGCCTGCAGCTGCCTGGGGCACGCTCTTAG >OriGene 5' read for mutant NM_006285 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCCGCGGCAAGCCA GCCCGCCGGCGCCCCGGAGCGCTGCCGCCACCGTCACCGCCACCGCCCGGTCCAGGCCCAAGCCCAGGCC CTGCGCGGAGCGGAGCGGCGCGGCGTCCACCCGGGCCCAGCCAGCCGGCGCGGCGGGGGCGGGTCCAGGA TCTTCGCGGATCTTCGCGATCTTCCATTCTCAGGCGGGAGCCGGAGTCCGGGCGCCCGGGATGGGCTGGG CCCGGCCATGGCAAGCGCGGCCTGCCCGGGCCCGGGGGACCCTGCCCTGGGTGAAGGCAGGGCCCCGGCC TGGGGGCCCCGGCCCCGGGCCGGGAAACCCCCCCCACCTGGCGGGGGCCCGGGAAACGGGATTTTAAAAA GTTAAGGAACCACGTCCCCCCAGATTTTGCTCTTAAGAATTCGAAAAACCGGCCAAAT >SC323413 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMMSRSGWMYYWCGTGCGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTT TAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCCGCGGC AAGCCAGCCCGCCGGCGCCCCGGAGCGCTGCCGCCACCGTCACCGCCACCGCCCGGTCCAGGCCCAAGCC CAGGCCCTGCGCGGAGCGGAGCGGCGCGGCGTCCACCCGGGCCCA |
| Restriction Sites | Please inquire |
| ACCN | NM_006285 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006285.2, NP_006276.2 |
| RefSeq Size | 2559 bp |
| RefSeq ORF | 1881 bp |
| Locus ID | 7016 |
| UniProt ID | Q15569 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain. Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases (LIMKs). The encoded protein can phosphorylate myelin basic protein and histone in vitro. The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that this gene plays an important role at and after the meiotic phase of spermatogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207560 | TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) |
CNY 4,664.00 |
|
| RC207560L1 | Lenti-ORF clone of TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) |
CNY 7,064.00 |
|
| RC207560L2 | Lenti-ORF clone of TESK1 (mGFP-tagged)-Human testis-specific kinase 1 (TESK1) |
CNY 6,460.00 |
|
| RC207560L3 | Lenti-ORF clone of TESK1 (Myc-DDK-tagged)-Human testis-specific kinase 1 (TESK1) |
CNY 6,460.00 |
|
| RC207560L4 | Lenti-ORF clone of TESK1 (mGFP-tagged)-Human testis-specific kinase 1 (TESK1) |
CNY 6,460.00 |
|
| RG207560 | TESK1 (tGFP-tagged) - Human testis-specific kinase 1 (TESK1) |
CNY 5,040.00 |
|
| SC116192 | TESK1 (untagged)-Human testis-specific kinase 1 (TESK1) |
CNY 4,672.00 |
|
| SC123458 | TESK1 (untagged)-Human testis-specific kinase 1 (cDNA clone MGC:45208 IMAGE:5170888), complete cds |
CNY 6,248.00 |
