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NEK9 (NM_033116) Human Untagged Clone
CAT＃: SC323388
NEK9 (untagged)-Kinase deficient mutant (K81M) of Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9)
CNY 7,176.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APUG; LCCS10; NC; NERCC; NERCC1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323388 sequence for NM_033116 edited (data generated by NextGen Sequencing)
ATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTTGGGAGC GAGTCCGGGGGTTGCGGGGACTCGAGTCCGGGGCCTAGCGCCAGTCAGGGGCCGCGAGCC GGCGGCGGCGCGGCGGAGCAGGAGGAACTGCACTACATCCCCATCCGCGTCCTGGGCCGC GGCGCCTTCGGGGAAGCCACGCTGTACCGCCGCACCGAGGATGACTCACTGGTTGTGTGG ATGGAAGTCGATTTGACCCGGCTGTCTGAGAAGGAACGTCGTGATGCCTTGAATGAGATT GTTATTCTGGCACTGCTGCAGCATGACAACATTATTGCCTACTACAATCACTTCATGGAC AATACCACGCTGCTGATTGAGCTGGAATATTGTAATGGAGGGAACCTGTATGACAAAATC CTTCGTCAGAAGGACAAGTTGTTTGAGGAAGAGATGGTGGTGTGGTACCTATTTCAGATT GTTTCAGCAGTGAGCTGCATCCATAAAGCTGGAATCCTTCATAGAGATATAAAGACATTA AATATTTTTCTGACCAAGGCAAACCTGATAAAACTTGGAGATTATGGCCTAGCAAAGAAA CTTAATTCTGAGTATTCCATGGCTGAGACGCTTGTGGGAACCCCATATTACATGTCTCCA GAGCTCTGTCAAGGAGTAAAGTACAATTTCAAGTCTGATATCTGGGCAGTTGGCTGCGTC ATTTTTGAACTGCTTACCTTAAAGAGGACGTTTGATGCTACAAACCCACTTAACCTGTGT GTGAAGATCGTGCAAGGAATTCGGGCCATGGAAGTTGACTCTAGCCAGTACTCTTTGGAA TTGATCCAAATGGTTCATTCGTGCCTTGACCAGGATCCTGAGCAGAGACCTACTGCAGAT GAACTTCTAGATCGCCCTCTTCTCAGGAAACGCAGGAGAGAGATGGAGGAAAAAGTCACT CTGCTTAATGCACCTACAAAGAGACCAAGGTCAAGCACTGTGACTGAAGCACCCATTGCT GTAGTAACATCACGAACCAGTGAAGTCTATGTTTGGGGTGGTGGAAAATCCACCCCCCAG AAACTGGATGTTATCAAGAGTGGCTGTAGTGCCCGGCAGGTCTGTGCAGGGAATACCCAC TTTGCTGTGGTCACAGTGGAGAAGGAACTGTACACTTGGGTGAACATGCAAGGAGGCACT AAACTCCATGGTCAGCTGGGCCATGGAGACAAAGCCTCCTATCGACAGCCAAAGCATGTG GAAAAGTTGCAAGGCAAAGCTATCCATCAGGTGTCATGTGGTGATGATTTCACTGTCTGT GTGACTGATGAGGGTCAGCTCTATGCCTTCGGATCAGATTATTATGGCTGCATGGGGGTG GACAAAGTTGCTGGCCCTGAAGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAAT CCAGTGGAGCAGGTCTCCTGTGGAGATAATCATGTGGTGGTTCTGACACGAAACAAGGAA GTCTATTCTTGGGGCTGTGGCGAATATGGACGACTGGGTTTGGATTCAGAAGAGGATTAT TATACACCACAAAAGGTGGATGTTCCCAAGGCCTTGATTATTGTTGCAGTTCAATGTGGC TGTGATGGGACATTTCTGTTGACCCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAA TTCAATAAGCTGGGTCTGAATCAGTGCATGTCGGGAATTATCAACCATGAAGCATACCAT GAAGTTCCCTACACAACGTCCTTTACCTTGGCCAAACAGTTGTCCTTTTATAAGATCCGT ACCATTGCCCCAGGCAAGACTCACACAGCTGCTATTGATGAGCGAGGCCGGCTGCTGACC TTTGGCTGCAACAAGTGTGGGCAGCTGGGCGTTGGGAACTACAAGAAGCGTCTGGGAATC AACCTGTTGGGGGGACCCCTTGGTGGGAAGCAAGTGATCAGGGTCTCCTGCGGTGATGAG TTTACCATTGCTGCCACTGATGATAATCACATTTTTGCCTGGGGCAATGGTGGTAATGGC CGCCTGGCAATGACCCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCT CGGCCTATTTTTGGATCTCTGCATCATGTCCCGGACCTGTCTTGCCGTGGATGGCATACC ATTCTCATCGTTGAGAAAGTATTGAATTCTAAGACCATCCGTTCCAATAGCAGTGGCTTA TCCATTGGAACTGTGTTTCAGAGCTCTAGCCCGGGAGGAGGCGGCGGGGGCGGCGGTGGT GAAGAAGAGGACAGTCAGCAGGAATCTGAAACTCCTGACCCAAGTGGAGGCTTCCGAGGA ACAATGGAAGCAGACCGAGGAATGGAAGGTTTAATCAGTCCCACAGAGGCCATGGGGAAC AGTAATGGGGCCAGCAGCTCCTGTCCTGGCTGGCTTCGAAAGGAGCTGGAAAATGCAGAA TTTATCCCCATGCCTGACAGCCCATCTCCTCTCAGTGCAGCGTTTTCAGAATCTGAGAAA GATACCCTGCCCTATGAAGAGCTGCAAGGACTCAAAGTGGCCTCTGAAGCTCCTTTGGAA CACAAACCCCAAGTAGAAGCCTCGTCACCTCGGCTGAATCCTGCAGTAACCTGTGCTGGG AAGGGAACACCACTGACTCCTCCTGCGTGTGCGTGCAGCTCTCTGCAGGTGGAGGTTGAG AGATTGCAGGGTCTGGTGTTAAAGTGTCTGGCTGAACAACAGAAGCTACAGCAAGAAAAC CTCCAGATTTTTACCCAACTGCAGAAGTTGAACAAGAAATTAGAAGGAGGGCAGCAGGTG GGGATGCATTCCAAAGGAACTCAGACAGCAAAGGAAGAGATGGAAATGGATCCAAAGCCT GACTTAGATTCAGATTCCTGGTGCCTCCTGGGAACAGACTCCTGTAGACCCAGCCTCTAG Clone variation with respect to NM_033116.4 242 a=>t;324 c=>t;1286 g=>a >OriGene 5' read for mutant NM_033116 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGGGCGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACGGATCCGCGGG CCGGCATCTGAAGCGAGCGGGACGCAGCGCGGCCAGGGCCTCCGGGCATACGCAGGCTGGTCCCCAAGGC CCGCGGCCGCCGCCATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTTGG GAGCGAGTCCGGGGGTTGCGGGGACTCGAGTCCGGGGCCTAGCGCCAGTCAGGGGCCGCGATCTTGTTTG TTGGTGCGGCGGAGCAGGAGGAACTGCACTACATCCCCATCCCGCGTCCTGGGCCGCGGCGCCGTCGGGG AAGCCACCGCTTGTACCGGCCGCACCGAGGATGACTCACTGGTTGGTGTGGATGGAAGTCGATTTGACCC GGCTTGTCTGAGAGGAACGTCGTTGAGCCTTGATGAGATTGTTATTCCTGGCACTGCTGGAGCTGGCCAC ATATTGCTAACTACATCACTTCATGGCCATACCAGCGCTGTGGATGACCTGAAAATGTAATGGAGGAACC GTGTAGACAATCTCTTCTCAAGGACATTTGTTGAGAAAGGGTGTGGACCATTCAATGTTACACAGACTGT ACTAAAGCTGAATCTTAGGAGATCAGACCATACTTTCTGCACGGAAACGTAACTGGCATTGCTTACGGAT CATTTTCGATTTTTCTGCGAACCTTGGACCTTATATGTTCC >SC323388 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACGGATCCGCGGGCCGGCAT CTGAAGCGAGCGGGACGCAGCGCGGCCAGGGCCTCCGGGCATACGCAGGCTGGTCCCCAAGGCCCGCGGC CGCCGCCATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTC |
| Restriction Sites | Please inquire |
| ACCN | NM_033116 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033116.3, NP_149107.3 |
| RefSeq Size | 3244 bp |
| RefSeq ORF | 2940 bp |
| Locus ID | 91754 |
| UniProt ID | Q8TD19 |
| Domains | RCC1, pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211326 | NEK9 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9) |
CNY 7,168.00 |
|
| RC211326L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9), Myc-DDK-tagged |
CNY 9,568.00 |
|
| RC211326L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9), mGFP tagged |
CNY 5,890.00 |
|
| RC211326L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211326L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9), mGFP tagged |
CNY 5,890.00 |
|
| RG211326 | NEK9 (tGFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9) |
CNY 8,768.00 |
|
| SC111489 | NEK9 (untagged)-Human NIMA (never in mitosis gene a)- related kinase 9 (NEK9) |
CNY 7,176.00 |
