STK23 (SRPK3) (NM_014370) Human Untagged Clone
CAT#: SC323379
SRPK3 (untagged)-Kinase deficient mutant (K108M) of Human SFRS protein kinase 3 (SRPK3)
CNY 4,232.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | MSSK-1; MSSK1; STK23 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_014370, the custom clone sequence may differ by one or more nucleotides
ATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGTAGCAGCAGCTCACAGGCCT CCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACACCGGTGCCTCAGATGCTGCAGGGCCT TCTGGGCTCCGACGACGAGGAACAGGAAGACCCCAAAGACTACTGCAAGGGCGGCTACCACCCTGTGAAG ATCGGCGACGTGTTCAATGGGCGGTACCACGTGGTGCGCAAACTGGGCTGGGGCCACTTCTCCACCGTCT GGCTCTGCTGGGACATCCAGCGCAAGCGCTTTGTGGCCCTCAAAGTGGTGAAGAGTGCGGGGCATTACAC GGAGACAGCTGTGGATGAGATCAAGCTCCTGAAATGTGTCCGGGACAGCGACCCCAGTGACCCCAAAAGA GAGACCATTGTCCAGCTCATTGATGACTTCAGGATCTCAGGAGTCAATGGAGTCCATGTGTGCATGGTGC TGGAGGTGCTGGGCCACCAGCTCCTCAAATGGATCATCAAGTCCAACTACCAGGGCCTGCCCGTGCCCTG CGTGAAGAGCATCGTGAGGCAGGTGCTGCACGGCCTGGACTACCTCCACACCAAGTGCAAGATCATCCAC ACGGACATCAAGCCCGAGAACATCTTGCTGTGTGTGGGGGACGCTTACATCAGGCGCCTGGCTGCCGAGG CCACGGAGTGGCAACAGGCAGGGGCGCCGCCCCCCTCCCGCTCCATAGTCAGCACTGCCCCCCAGGAGGT CTTGCAGACCGGTAAGCTGTCCAAAAACAAGAGGAAGAAGATGAGGCGCAAACGGAAACAGCAGAAGCGG CTGCTGGAGGAGCGGCTGCGGGACCTGCAGAGGCTGGAGGCCATGGAGGCTGCCACCCAGGCTGAGGACT CTGGCTTGAGACTAGACGGGGGCAGCGGCTCCACATCCTCTTCAGGCTGTCACCCCGGGGGCGCCAGAGC AGGTCCCTCCCCAGCCTCTTCCTCCCCCGCCCCAGGGGGCGGCCGTAGCCTCAGCGCGGGCTCACAGACC TCAGGCTTCTCCGGCTCCCTCTTCTCTCCTGCCTCCTGCTCCATCCTCTCCGGCTCGTCCAATCAGCGAG AGACCGGGGGCCTCCTGTCGCCTAGCACACCATTCGGTGCCTCGAACCTCCTGGTGAACCCCCTGGAGCC CCAAAATGCAGATAAGATCAAGATCAAGATCGCAGACCTGGGCAACGCCTGCTGGGTGCACAAGCACTTC ACGGAAGACATCCAGACTCGGCAGTACCGGGCCGTCGAGGTGCTGATCGGCGCCGAATACGGCCCCCCGG CAGACATCTGGAGCACAGCCTGCATGGCCTTCGAGCTGGCCACTGGTGACTACCTGTTCGAGCCGCATTC TGGAGAAGACTACAGTCGTGATGAGGACCACATCGCTCACATAGTGGAGCTTCTGGGGGACATCCCCCCA GCCTTCGCCCTCTCAGGCCGCTATTCCCGGGAGTTCTTCAACCGGAGAGGAGAGCTGCGGCACATCCACA ATCTCAAGCACTGGGGCCTGTACGAGGTACTCATGGAAAAGTACGAGTGGCCCCTAGAGCAGGCCACACA GTTCAGCGCCTTTCTGCTGCCCATGATGGAGTACATCCCCGAAAAGCGGGCCAGTGCCGCTGACTGCCTC CAGCACCCCTGGCTCAACCCCTAG >OriGene 5' read for mutant NM_014370 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGGGGCAC CGGAGCTGCGGGCTGCGTGGCCGGGATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGC GGCAGTAGCAGCAGCTCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACAC CGGTGCCTCAGATGCTGCAGGGGCCTTTCTGGGCTCCGACGACGAGGGAACAGGGAAAGACCCCAAAGAA CTACTGCAAGGGCGGGCTACCCACCCCTGTTGAAAGAATCGGGCGACGTGGTTCATGGGGGCGGGTCCCA CGTGGGTGGGGGCAAAACTGGGCCTGGGGCCATTTTCTCCACCCGTCCTGGCTTGGCCTGGGACATCCCA CCGCCAGGCCCCTTTGGGGCCCCTCATTTTGTTAAAAAATTCCGGGCCATTTACACGGAAAAGGTTTGGG GAAAAAAATTAACCCCCCGAAAATTGGTCCCGGAAACCCGAACCCCCTGGGCCCCAAAAAAAAAAAAACA TTTGGCCCATCTATTTGGGAAATTTTCGGGTTTCCCGGGTTTTAAGGGGGCCCCGGGGGGCCCAGGGCCC CGAAAATCTCCTCCCCCCCCCCCCCCTAAAGGGGTATTATAGTTGCCACTTCAACAGGGCCCTTCCCTTC CCCCGGGTAGAAAAATACTCAGGGGGCGAGGGTGCTCGACGCTCTGGAACTCCCCCACCCCATTTGTGCG ATATTTTCTCCCGCGGAGGTATTCGCCACGAGAAACTTGTTGAGGGTGGGGGGGATCATAATATAGGGAC GCGTGATAGTGGCGACCGCAGATGATACAGAGAGGCGGGAGCCCCTCTCACATGATGCTAGACTCATTCT CTGCAAGGGAGATTG >SC323379 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGCCCGGGGCACCGGAGC TGCGGGCTGCGTGGCCGGGATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGT AGCAGCAGCTCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCC |
Restriction Sites | Please inquire |
ACCN | NM_014370 |
Insert Size | 2300 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014370.2, NP_055185.2 |
RefSeq Size | 2040 bp |
RefSeq ORF | 1704 bp |
Locus ID | 26576 |
UniProt ID | Q9UPE1 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215496 | SRPK3 (Myc-DDK-tagged)-Human SRSF protein kinase 3 (SRPK3), transcript variant 1 |
CNY 4,224.00 |
|
RC215496L1 | Lenti ORF clone of Human SRSF protein kinase 3 (SRPK3), transcript variant 1, Myc-DDK-tagged |
CNY 6,624.00 |
|
RC215496L2 | Lenti ORF clone of Human SRSF protein kinase 3 (SRPK3), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
RC215496L3 | Lenti ORF clone of Human SRSF protein kinase 3 (SRPK3), transcript variant 1, Myc-DDK-tagged |
CNY 6,624.00 |
|
RC215496L4 | Lenti ORF clone of Human SRSF protein kinase 3 (SRPK3), transcript variant 1, mGFP tagged |
CNY 6,624.00 |
|
RG215496 | SRPK3 (tGFP-tagged) - Human SRSF protein kinase 3 (SRPK3), transcript variant 1 |
CNY 5,824.00 |
|
SC124124 | SRPK3 (untagged)-Human SRSF protein kinase 3 (SRPK3), transcript variant 1 |
CNY 4,232.00 |