United States
Germany
Japan
United Kingdom
China
CSNK1G2 (NM_001319) Human Untagged Clone
CAT＃: SC323375
CSNK1G2 (untagged)-Kinase deficient mutant (K75M) of Human casein kinase 1, gamma 2 (CSNK1G2)
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CK1g2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001319, the custom clone sequence may differ by one or more nucleotides
ATGGATTTTGACAAGAAAGGAGGGAAAGGGGAGACGGAGGAGGGCCGGAGAATGTCCAAGGCCGGCGGGG GCCGGAGCAGCCACGGCATCCGGAGCTCGGGGACCAGCTCGGGGGTCCTGATGGTGGGCCCCAACTTCCG CGTCGGCAAGAAGATCGGCTGCGGCAACTTCGGGGAGCTCCGCCTAGGAAAGAATCTCTATACAAATGAA TACGTGGCTATCAAATTGGAGCCGATCAAGTCCCGGGCCCCGCAGCTGCACCTGGAGTACCGGTTCTACA AGCAGCTCAGCGCCACAGAGGGCGTCCCTCAGGTCTACTACTTCGGTCCGTGCGGGAAGTACAACGCCAT GGTGCTGGAGCTGCTGGGGCCCAGCCTGGAGGACCTGTTCGACCTGTGCGACCGGACCTTCACGCTCAAG ACGGTGCTGATGATCGCCATCCAGCTGATCACGCGCATGGAGTATGTGCACACCAAGAGCCTAATCTACC GGGACGTGAAGCCCGAGAACTTCCTGGTGGGCCGCCCGGGGACCAAGCGGCAGCATGCCATCCACATCAT CGACTTCGGGCTGGCCAAGGAGTACATCGACCCCGAGACCAAGAAGCACATCCCGTACCGCGAGCACAAG AGCCTGACGGGCACGGCGCGCTACATGAGCATCAACACGCACCTGGGCAAGGAGCAGAGCCGCCGCGACG ACCTGGAGGCGCTGGGCCACATGTTCATGTACTTCCTGCGCGGCAGCCTCCCCTGGCAGGGGCTCAAGGC CGACACGCTCAAGGAGCGGTACCAGAAGATCGGGGACACCAAACGCGCCACGCCCATCGAGGTGCTCTGC GAGAACTTCCCAGAGGAGATGGCCACGTACCTGCGCTATGTGCGGCGCCTGGACTTCTTCGAGAAGCCCG ACTATGACTACCTGCGGAAGCTCTTCACCGACCTCTTCGACCGCAGTGGCTTCGTGTTCGACTATGAGTA CGACTGGGCCGGGAAGCCCCTGCCGACCCCCATCGGCACCGTCCACACCGACCTGCCCTCCCAGCCTCAG CTCCGGGACAAAACCCAGCCGCACAGCAAAAACCAGGCGTTGAACTCCACCAACGGGGAGCTGAATGCGG ACGACCCCACGGCCGGCCACTCCAACGCCCCGATCACAGCGCCTGCAGAGGTGGAGGTGGCCGATGAAAC CAAATGCTGCTGTTTCTTCAAGAGGAGAAAGAGAAAATCGCTGCAGCGACACAAGTGA >OriGene 5' read for mutant NM_001319 unedited
TCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGAATGTCTCCT GCCCCCGAGAGCGACCCCGAGGCCACTGAGAAGAGCAGCGCGGCCTGGCCGGCCCGAACGCCTGCGTCTC AGTAGCTGGGAGCCACGGGCCCACGCCCGCCCACCGGCCGCAGTGATGTTCTAGCCACAGAGGAGCCAAG ACCTCAGTTTCCAGAGACTTGGGATTTGCACGGCAGCAGAGTCACCGTGGAGAGGCCAGGGGTATCACAA ACTTTATGGATTTTGACAAGAAAAGGAGGGGAAAGGGGAGAACGGAGGGAAGGGCCCGGAAAAAGGTCCA AGGCCCGGGGGGGGCCGAAACCACCCACCGGAATTCCGGAACTTTCGGGAAACCACTTTCGGGGGGTCCG GGAGGGGGGGGCCCCCACCTCCCCCGTTTCGGGCAAAAAAAATCGCCGGGGGGAAACTCGGGGGAACCCC CCCCCAAAGAAAAAAATTCTTCTATACAATGAAAAATCCGTGGCCTTTAAGTTTTGGGACCCATTTAATT CCCGGGGGCCAAAAGATTTAAGAAAATGGGGGTTTTTACTTTTCCCCCCGGGGCCGCAGATGTTTAACTC GTAAGGGGCCCCCCTCGTGTGGGCGTGGACCCCTTTTTTTAAACAGGAATCCCCCAGGCGCCCCGCCCGG GGGGGCACACCCGGGGGAAAATGGTTTTAAACCTTGGGGAACACGGAGCTTTTTGCGCCTAAAAGAGTGT GGGTGAGTTTGTGCTCCTCTCCATGTTTCATTCCGCCCCGCGAGGGATATATGGTCGCCCACGCAGACTC TACATTATACTCGCGCGGTGAGGATGGGACACGGAATATATTCTCTCTGTGTGCGGGCCCGCGCGACGCG A >SC323375 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGAATGTCTCCTGCCCCC GAGAGCGACCCCGAGGCCACTGAGAAGAGCAGCGCGGCCTGGCCGGCCCGAACGCCTGCGTCTCAGTAGC TGGGAGCCACGGGCCCACGCCCGCCCACCGGCCGCAGTGATGTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_001319 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001319.5, NP_001310.2 |
| RefSeq Size | 2446 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 1455 |
| UniProt ID | P78368 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Hedgehog signaling pathway |
| Gene Summary | Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202145 | CSNK1G2 (Myc-DDK-tagged)-Human casein kinase 1, gamma 2 (CSNK1G2) |
CNY 3,656.00 |
|
| RC202145L1 | Lenti ORF clone of Human casein kinase 1, gamma 2 (CSNK1G2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202145L2 | Lenti ORF clone of Human casein kinase 1, gamma 2 (CSNK1G2), mGFP tagged |
CNY 5,890.00 |
|
| RC202145L3 | Lenti ORF clone of Human casein kinase 1, gamma 2 (CSNK1G2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202145L4 | Lenti ORF clone of Human casein kinase 1, gamma 2 (CSNK1G2), mGFP tagged |
CNY 5,890.00 |
|
| RG202145 | CSNK1G2 (tGFP-tagged) - Human casein kinase 1, gamma 2 (CSNK1G2) |
CNY 4,370.00 |
|
| SC119302 | CSNK1G2 (untagged)-Human casein kinase 1, gamma 2 (CSNK1G2) |
CNY 2,950.00 |
其它CSNK1G2产品
