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IKBKE (NM_014002) Human Untagged Clone
CAT＃: SC323370
IKBKE (untagged)-Kinase deficient mutant (K38M) of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE)
CNY 5,256.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IKK-E; IKK-i; IKKE; IKKI |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323370 sequence for NM_014002 edited (data generated by NextGen Sequencing)
ATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAGGGGGCCACT GCCAGTGTGTACAAGGCCCGCAACAAGAAATCCGGAGAGCTGGTTGCTGTGATGGTCTTC AACACTACCAGCTACCTGCGGCCCCGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGG AAGCTGAACCACCAGAACATCGTCAAGCTCTTTGCGGTGGAGGATACGGGCGGAAGCCGG CAGAAGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTGGAGAGC CCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCGCTGTGTGGTG GCCGGCATGAACCACCTGCGGGAGAACGGCATTGTGCATCGCGACATCAAGCCGGGGAAC ATCATGCGCCTCGTAGGGGAGGAGGGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCT GCCCGGGAGCTGGATGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTG CATCCCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTG ACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGCCACTGGCAGCCTGCCC TTCATCCCCTTTGGTGGGCCACGGCGGAACAAGGAGATCATGTACCGGATCACCACGGAG AAGCCGGCTGGGGCCATTGCAGGTGCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGC TACACCCTCCCCATCACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATC CTGGCCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTCTTTGCG GAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCCCAGGCAGTC CTGCACCACATCTATATCCATGCCCACAACACGATAGCCATTTTCCAGGAGGCCGTGCAC AAGCAGACCAGTGTGGCCCCCCGACACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTC CTCGAGCCCAGCGTCTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACC CTCTTCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGACGTCCCC AAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGCCAAGGGCGTGTTGGGC GCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGATGGGCAGGAGCTAATGTTT CGGGGGCTGCACTGGGTCATGGAGGTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTG GCAAGGACATCCCTCCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTG GCTGGAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGGCTGCGG ACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGAGACCCAGGAGAGCCTG AGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGGATCAGGTACATGAGGACAGAAGCATC CAGCAGATTCAGTGCTGTTTGGACAAGATGAACTTCATCTACAAACAGTTCAAGAAGTCT AGGATGAGGCCAGGGCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAAT TTCAGTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGAAGTAT CAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCACGAGACCAGGAACCAC CTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTAACACAGAAGCCCAGGGGGTCCAGGAG AGTCTCAGCAAGCTCCTGGAAGAGCTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGG GCTCAGGCCTCGCCGCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTT CTCCACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTGGACAAC AACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACTTCCTGATGTCTGA Clone variation with respect to NM_014002.3 113 a=>t;201 t=>c;225 g=>t;2138 c=>t >OriGene 5' read for mutant NM_014002 unedited
CCCGCCCGTTCGAGCAACTGGGCGGTAGGCGCTGTACGGTCTGTGGAGGTCTATATAAGCAGAGCTCGTT TAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAGAAACC CACTAGTCCCAGCTCCTGGGGTGGCACAGACATTGCAACTGGCCCTGCCTGTGGGTCCTAGGGGCCCTTG GCTACCAGGAGGCTAAGAACACTGCTCATGAATGACAGTGAGCCCTGAAAGCTCTGGGGGTGTCACCCAG TCCCACAAGCCTGCATCCCCTGCAGTGGAGATGGGCTCAGCTCCTGGACGTGCCACAGACAGAAAGCATA ACATACAGTTCGCCAGGAGAGCCTTTGCCTGACTCAGGGCAGCTTAAAGTTGGGGGCAGAAGTGACAGCC AGCTCAGGGCAGAAATGCAAAGCCAGCAATTACTGGTGGCAACGATAACTGGCGGGGCAGGGGCCCTGCA TTGTGTAAAGGCCGCAAAGAAATCGAAAACTGTTTGCTGTATGGTTTAAAATACAGCTACTGGGCCCCGA GGGTGAGTGAAGGATTTAAGGCCTGGGAACTAACCCCGAAACTCTCAGCTTTCGTGAGAGACCGGCGAAC CGCAAAGCTGTGTGAGACTCTCCCTGGACCCTGAGTGTGTGAACCGGAAGCCTGGTCGCAGAGATACCTG TGTCGCCTGTGGCGCGTACACTGGGAACGCTGCTCTGCTAACGGACATGCGTAGAGATGTACACTACAAA AAATGCGCGAAGGCGGT >SC323370 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CKACTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAGAAACCCACTAGTCCCA GCTCCTGGGGTGGCACAGACATTGCAACTGGCCCTGCCTGTGGGTCCTAGGGGCCCTTGGCTACCAGGAG GCTAAGAACACTGCTCATGAATGACAGTGAGCCCTGAAAGCTCTG |
| Restriction Sites | Please inquire |
| ACCN | NM_014002 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014002.2, NP_054721.1 |
| RefSeq Size | 3263 bp |
| RefSeq ORF | 2151 bp |
| Locus ID | 9641 |
| UniProt ID | Q14164 |
| Domains | pkinase |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway |
| Gene Summary | IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009] Transcript Variant: This variant (1) is the longest transcript and it encodes the longest protein (isoform 1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212481 | IKBKE (Myc-DDK-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1 |
CNY 5,248.00 |
|
| RC212481L3 | Lenti ORF clone of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1, Myc-DDK-tagged |
CNY 7,648.00 |
|
| RC212481L4 | Lenti ORF clone of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1, mGFP tagged |
CNY 7,648.00 |
|
| RG212481 | IKBKE (tGFP-tagged) - Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1 |
CNY 6,848.00 |
|
| SC115206 | IKBKE (untagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1 |
CNY 5,256.00 |
