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GAK (NM_005255) Human Untagged Clone
CAT＃: SC323361
GAK (untagged)-Kinase deficient mutant (K69M) of Human cyclin G associated kinase (GAK)
CNY 9,136.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DNAJ26; DNAJC26 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005255, the custom clone sequence may differ by one or more nucleotides
ATGTCGCTGCTGCAGTCGGCGCTCGACTTCTTGGCGGGTCCAGGCTCCCTGGGCGGTGCTTCCGGCCGCG ACCAGAGTGACTTCGTGGGGCAGACGGTGGAACTGGGCGAGCTGCGGCTGCGGGTGCGGCGGGTCCTGGC CGAAGGAGGGTTTGCATTTGTGTATGAAGCTCAAGATGTGGGGAGTGGCAGAGAGTATGCATTAAAGAGG CTATTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAAGAAGTTTGCTTCATGAAAAAGCTTTCCG GCCACCCGAACATTGTCCAGTTTTGTTCTGCAGCGTCTATAGGAAAAGAGGAGTCAGACACGGGGCAGGC TGAGTTCCTCTTGCTCACAGAGCTCTGTAAAGGGCAGCTGGTGGAATTTTTGAAGAAAATGGAATCTCGA GGCCCCCTTTCGTGCGACACGGTTCTGAAGATCTTCTACCAGACGTGCCGCGCCGTGCAGCACATGCACC GGCAGAAGCCGCCCATCATCCACAGGGACCTCAAGGTTGAGAACTTGTTGCTTAGTAACCAAGGGACCAT TAAGCTGTGTGACTTTGGCAGTGCCACGACCATCTCGCACTACCCTGACTACAGCTGGAGCGCCCAGAGG CGAGCCCTGGTGGAGGAAGAGATCACGAGGAATACAACACCAATGTATAGAACACCAGAAATCATAGACT TGTATTCCAACTTCCCGATCGGCGAGAAGCAGGATATCTGGGCCCTGGGCTGCATCTTGTACCTGCTGTG CTTCCGGCAGCACCCTTTTGAGGATGGAGCGAAACTTCGAATAGTCAATGGGAAGTACTCGATCCCCCCG CACGACACGCAGTACACGGTCTTCCACAGCCTCATCCGCGCCATGCTGCAGGTGAACCCGGAGGAGCGGC TGTCCATCGCCGAGGTGGTGCACCAGCTGCAGGAGATCGCGGCCGCCCGCAACGTGAACCCCAAGTCTCC CATCACAGAGCTCCTGGAGCAGAATGGAGGCTACGGGAGCGCCACACTGTCCCGAGGGCCACCCCCTCCC GTGGGCCCCGCTGGCAGTGGCTACAGTGGAGGCCTGGCGCTGGCGGAGTACGACCAGCCGTATGGCGGCT TCCTGGACATTCTGCGGGGTGGGACAGAGCGGCTCTTCACCAACCTCAAGGACACCTCCTCCAAGGTCAT CCAGTCCGTCGCTAATTATGCAAAGGGTGACCTGGACATATCTTACATCACATCCAGAATTGCAGTGATG TCATTCCCAGCAGAAGGTGTGGAGTCAGCGCTCAAAAACAACATCGAAGATGTGCGGTTGTTCCTGGACT CCAAGCACCCAGGGCACTATGCCGTCTACAACCTGTCCCCGAGGACCTACCGGCCCTCCAGGTTCCACAA CCGGGTCTCCGAGTGTGGCTGGGCAGCACGGCGGGCCCCACACCTGCACACCCTGTACAACATCTGCAGG AACATGCACGCCTGGCTGCGGCAGGACCACAAGAACGTCTGCGTCGTGCACTGCATGGACGGGAGAGCCG CGTCTGCTGTGGCCGTCTGCTCCTTCCTGTGCTTCTGCCGTCTCTTCAGCACCGCGGAGGCCGCCGTGTA CATGTTCAGCATGAAGCGCTGCCCACCAGGCATCTGGCCATCCCACAAAAGGTACATCGAGTACATGTGT GACATGGTGGCGGAGGAGCCCATCACACCCCACAGCAAGCCCATCCTGGTGAGGGCCGTGGTCATGACAC CCGTGCCGCTGTTCAGCAAGCAGAGGAGCGGCTGCAGGCCCTTCTGCGAGGTCTACGTGGGGGACGAGCG TGTGGCCAGCACCTCCCAGGAGTACGACAAGATGCGGGACTTTAAGATTGAAGATGGCAAAGCGGTGATT CCCCTGGGCGTCACGGTGCAAGGAGACGTGCTCATCGTCATCTATCACGCCCGGTCCACTCTGGGCGGCC GGCTGCAGGCCAAGATGGCATCCATGAAGATGTTCCAGATTCAGTTCCACACGGGGTTTGTGCCTCGGAA CGCCACCACTGTGAAATTTGCCAAGTATGACCTGGACGCGTGTGACATTCAAGAAAAATACCCGGATTTA TTTCAAGTGAACCTGGAAGTGGAGGTGGAGCCCAGGGACAGGCCGAGCCGGGAAGCCCCACCATGGGAGA ACTCGAGCATGAGGGGGCTGAACCCCAAAATCCTGTTTTCCAGCCGGGAGGAGCAGCAAGACATTCTGTC TAAGTTTGGGAAGCCGGAGCTTCCCCGGCAGCCTGGCTCCACGGCTCAGTATGATGCTGGGGCAGGGTCC CCGGAAGCCGAACCCACAGACTCTGACTCACCGCCAAGCAGCAGCGCGGACGCCAGTCGCTTCCTGCACA CGCTGGACTGGCAGGAAGAGAAGGAGGCAGAGACTGGTGCAGAAAATGCCTCTTCCAAGGAGAGCGAGTC TGCCCTGATGGAGGACAGAGACGAGAGTGAGGTGTCAGATGAAGGGGGATCCCCGATCTCCAGCGAGGGC CAGGAACCCAGGGCCGACCCAGAGCCCCCCGGCCTGGCAGCAGGGCTGGTGCAGCAGGACTTGGTTTTTG AGGTGGAGACACCGGCTGTGCTGCCAGAGCCTGTGCCACAGGAAGACGGGGTCGACCTCCTGGGCCTGCA CTCCGAGGTGGGCGCAGGGCCAGCTGTACCCCCGCAGGCCTGCAAGGCCCCCTCCAGCAACACCGACCTG CTCAGCTGCCTCCTTGGGCCCCCTGAGGCCGCCTCCCAGGGGCCCCCGGAGGATCTGCTCAGCGAGGACC CGCTGCTCCTGGCAAGCCCGGCCCCTCCCCTGAGCGTGCAGAGCACCCCAAGAGGAGGGCCCCCTGCCGC TGCTGACCCCTTTGGCCCGCTTCTGCCGTCTTCAGGCAACAACTCCCAGCCCTGCTCCAATCCTGATCTC TTCGGCGAATTTCTCAATTCGGACTCTGTGACCGTCCCACCATCCTTCCCGTCTGCCCACAGTGCTCCGC CCCCATCCTGCAGCGCCGACTTCCTGCACCTGGGGGATCTGCCAGGAGAGCCCAGCAAGATGACAGCCTC GTCCAGCAACCCAGACCTGCTGGGAGGATGGGCTGCCTGGACCGAGACTGCAGCATCGGCAGTGGCCCCC ACGCCAGCCACAGAAGGCCCCCTCTTCTCTCCTGGAGGTCAGCCGGCCCCTTGTGGCTCTCAGGCCAGCT GGACCAAGTCTCAGAACCCGGACCCATTTGCTGACCTTGGCGACCTCAGCTCCGGCCTCCAAGGCTCACC AGCTGGATTCCCTCCTGGGGGCTTCATTCCCAAAACGGCCACCACGCCCAAAGGCAGCAGCTCCTGGCAG ACAAGTCGGCCGCCAGCCCAGGGCGCCTCATGGCCCCCTCAGGCCAAGCCGCCCCCCAAAGCCTGCACAC AGCCAAGGCCTAACTATGCCTCGAACTTCAGTGTGATCGGGGCGCGGGAGGAGCGGGGGGTCCGCGCACC CAGCTTTGCTCAAAAGCCAAAAGTCTCTGAGAACGACTTTGAAGATCTGTTGTCCAATCAAGGCTTCTCC TCCAGGTCTGACAAGAAAGGGCCAAAGACCATTGCAGAGATGAGGAAGCAGGACCTGGCTAAAGACACGG ACCCACTCAAGCTGAAGCTCCTGGACTGGATTGAGGGCAAGGAGCGGAACATCCGGGCCCTGCTGTCCAC GCTGCACACAGTGCTGTGGGACGGGGAGAGCCGCTGGACGCCCGTGGGCATGGCCGACCTGGTGGCTCCG GAGCAAGTGAAGAAGCACTATCGCCGCGCGGTGCTGGCTGTGCACCCCGACAAGGCTGCGGGGCAGCCGT ACGAGCAGCACGCCAAGATGATCTTCATGGAGCTGAATGACGCCTGGTCGGAGTTTGAGAACCAGGGCTC CCGGCCCCTCTTCTGA >OriGene 5' read for mutant NM_005255 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCGGAAGATGGTGCA CCTCCGGGCCGGCGGTTGCTGAGCTGACCCGGACGGCGAGGGAGCGGGAGCCCGAGCCCGACCACTCCGG CTGCCGCGGGGTGCGGCGCAGCCACCGCCATGTCGCTGCTGCAGTCGGCGCTCGACTTCTTGGCGGGTCC AGGCTCCCTGGGCGGTGCTTCCGGCCGCGACCAGAGTGACTTCGTGGGGCAGACGGTGGAACTGGGCGTG CTGCGGCTGCGGGGTGCGGCGGGTCCTGCCCGAAGGAGGGTTTGCATTTGTGTATGAGCTCAGATGTGGG GAAGTGGCAGAAAGTATGCTTTATGGAGGCTATTATCCAATGGAAAGGAAAGAACCAAACCCTTCTTTCA GAAGTTTGCTTCTTGAAAAGCTTTCCGGCCCCCGACATTGTCCATTTTGTTTGAACCGTCTTAGGAAAAA GATTCAACACCGGGGCGGCTGATTTCTCTTCTCCAGCTTTGGAAAGGCGAGTGGGTGAATTTAAAAAATG GAAATCTAGGCCCCTTTTCGCACCGTTGAGATATTTTACAGAGTGCGCCTCGCACAGGCCGGGAAACCCA CATCACAGGCCCAGGTGAAATGTGTGTACCAGGACTATACTGTGTATTGGGAGCGCAACTGCTACTCGTA TCTTGACCACGAGCCGTGAGAAGATCCCGATATCCCGTAGAAACAAACCTTCTCTTGCCT >SC323361 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCTTGMGCATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCGGAAGATGGTGCACCTCCGGG CCGGCGGTTGCTGAGCTGACCCGGACGGCGAGGGAGCGGGAGCCCGAGCCCGACCACTCCGGCTGCCGCG GGGTGCGGCGCAGCCACCGCCATGTCGCTGCTGCAGTCGGCGCTC |
| Restriction Sites | Please inquire |
| ACCN | NM_005255 |
| Insert Size | 4910 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005255.1, NP_005246.1 |
| RefSeq Size | 4331 bp |
| RefSeq ORF | 3936 bp |
| Locus ID | 2580 |
| UniProt ID | O14976 |
| Domains | pkinase, TyrKc, DnaJ, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223572 | GAK (Myc-DDK-tagged)-Human cyclin G associated kinase (GAK) |
CNY 9,128.00 |
|
| RC223572L1 | Lenti ORF clone of Human cyclin G associated kinase (GAK), Myc-DDK-tagged |
CNY 11,528.00 |
|
| RC223572L2 | Lenti ORF clone of Human cyclin G associated kinase (GAK), mGFP tagged |
CNY 15,010.00 |
|
| RC223572L3 | Lenti ORF clone of Human cyclin G associated kinase (GAK), Myc-DDK-tagged |
CNY 15,010.00 |
|
| RC223572L4 | Lenti ORF clone of Human cyclin G associated kinase (GAK), mGFP tagged |
CNY 11,528.00 |
|
| RG223572 | GAK (tGFP-tagged) - Human cyclin G associated kinase (GAK) |
CNY 10,728.00 |
|
| SC116876 | GAK (untagged)-Human cyclin G associated kinase (GAK) |
CNY 9,136.00 |
