MELK (NM_014791) Human Untagged Clone
CAT#: SC323339
MELK (untagged)-Kinase deficient mutant (K40M) of Human maternal embryonic leucine zipper kinase (MELK)
CNY 4,856.00
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HPK38 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323339 sequence for NM_014791 edited (data generated by NextGen Sequencing)
ATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGT GGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAA ATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGGCC TTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACCATGTGCTAGAGACAGCCAAC AAAATATTCATGGTTCTTGAGTACTGCCCTGGAGGAGAGCTGTTTGACTATATAATTTCC CAGGATCGCCTGTCAGAAGAGGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTT GCTTATGTGCACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACCCAAGGGTAAC AAGGATTACCATCTACAGACATGCTGTGGGAGTCTGGCTTATGCAGCACCTGAGTTAATA CAAGGCAAATCATATCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATAT GTTCTTATGTGTGGATTTCTACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAG ATTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTT CAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCTATTGAACCAT CCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGCAAAGCAAGAATCCTTTTATT CACCTCGATGATGATTGCGTAACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACA ATGGAGGATTTAATTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTT CTAGCCAAGAAGGCTCGGGGAAAACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGA CAAGCCAGTGCTACCCCATTCACAGACATCAAGTCAAATAATTGGAGTCTGGAAGATGTG ACCGCAAGTGATAAAAATTATGTGGCGGGATTAATAGACTATGATTGGTGTGAAGATGAT TTATCAACAGGTGCTGCTACTCCCCGAACATCACAGTTTACCAAGTACTGGACAGAATCA AATGGGGTGGAATCTAAATCATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTA AAGAACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTACTTTATG TTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAATACTCACTACG CCAAATCGTTACACTACACCCTCAAAAGCTAGAAACCAGTGCCTGAAAGAAACTCCAATT AAAATACCAGTAAATTCAACAGGAACAGACAAGTTAATGACAGGTGTCATTAGCCCTGAG AGGCGGTGCCGCTCAGTGGAATTGGATCTCAACCAAGCACATATGGAGGAGACTCCAAAA AGAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTTATCACTGTG CTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCAGAAGACTAAAGCTTCAC TATAATGTGACTACAACTAGATTAGTGAATCCAGATCAACTGTTGAATGAAATAATGTCT ATTCTTCCAAAGAAGCATGTTGACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACA CAGTCAGATTTTGGGAAAGTGACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAA CCCGATGTGGTGGGTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTTTACAAAAGA TTAGTGGAAGACATCCTATCTAGCTGCAAGGTATAA Clone variation with respect to NM_014791.2 >OriGene 5' read for mutant NM_014791 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGCGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGTCTCTCAGG ACAGCAGGCCCCTGTCCTTCTGTCGGGCGCCGCTCAGCCGTGCCCTCCGCCCCTCAGGTCAGTTCTCCCG CCTGCCCGCCGCAGTCGCAGTTGATGGCTGGGGTCTGGGCTGTGCGGGGCGCAGCGGCCCAAACCCAGTT TGCTCCTGGCTCTCGGGAGACTGGAGGATTTCATCGGAGCCCCGCGCTTTACCAGCCCTGTTCCCTTGAT AAGATATTTGACCTTTCCGACCCGCGGTTTTCTTCTCCAATCTCGCTCCGTTGCCCAGGCTGGAGTGCAG TGGCGTGATCTCGGCTCACTGCAACCTCTGCCTCCTGCCTCAGCCTCCCAAGTTCTTTTTCTAATTCCAA ATAAACTTGCAAGAGGACTATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATT GGGACAGGTGGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAATGA TCATGGATAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGCCTTGAAGACCCTGA GACATCAGCATATATGTCAACTCTACCATGTGCTAGAGACAGCCCAACAAAATATTCATGGTCTGAGTAC TGCCCTGAGGAAGCTGTTTGACTTAATATTCCCAGATCGCCTGTCAGAAGAAGGAGACCCGGTTGTCTCG TCAGAATGTATTCTGCTGTGCTTATTGTGCACAGCAGCTATGCTACAGACCTCAGCAAAATTGCTGTTTT GATGAATATACTAAATAAGCTGATTAGACTTTGTCTCCTGTGTGCAAAC >SC323339 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGTCTCTCAGGACAGC AGGCCCCTGTCCTTCTGTCGGGCGCCGCTCAGCCGTGCCCTCCGCCCCTCAGGTCAGTTCTCCCGCCTGC CCGCCGCAGTCGCAGTTGATGGCTGGGGTCTGGGCTGTGCGGGGC |
Restriction Sites | Please inquire |
ACCN | NM_014791 |
Insert Size | 2700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014791.2, NP_055606.1 |
RefSeq Size | 2501 bp |
RefSeq ORF | 1956 bp |
Locus ID | 9833 |
UniProt ID | Q14680 |
Domains | pkinase, TyrKc, KA1, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204245 | MELK (Myc-DDK-tagged)-Human maternal embryonic leucine zipper kinase (MELK) |
CNY 7,272.00 |
|
RC204245L1 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), Myc-DDK-tagged |
CNY 9,672.00 |
|
RC204245L2 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), mGFP tagged |
CNY 9,672.00 |
|
RC204245L3 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204245L4 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), mGFP tagged |
CNY 5,890.00 |
|
RG204245 | MELK (tGFP-tagged) - Human maternal embryonic leucine zipper kinase (MELK) |
CNY 8,872.00 |
|
SC114824 | MELK (untagged)-Human maternal embryonic leucine zipper kinase (MELK) |
CNY 4,856.00 |