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PHKG1 (NM_006213) Human Untagged Clone
CAT＃: SC323335
PHKG1 (untagged)-Kinase deficient mutant (K49M) of Human phosphorylase kinase, gamma 1 (muscle) (PHKG1)
CNY 5,200.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHKG |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006213, the custom clone sequence may differ by one or more nucleotides
ATGACCCGGGACGAGGCACTGCCGGACTCTCATTCTGCACAGGACTTCTATGAGAATTATGAGCCCAAAG AGATCCTGGGCAGGGGCGTTAGCAGTGTGGTCAGGCGATGCATCCACAAGCCCACGAGCCAGGAGTACGC CGTGAAGGTCATCGACGTCACCGGTGGAGGCAGCTTCAGCCCGGAGGAGGTGCGGGAGCTGCGAGAAGCC ACGCTGAAGGAGGTGGACATCCTGCGCAAGGTCTCAGGGCACCCCAACATCATACAGCTGAAGGACACTT ATGAGACCAACACTTTCTTCTTCTTGGTGTTTGACCTGATGAAGAGAGGGGAGCTCTTTGACTACCTCAC TGAGAAGGTCACCTTGAGTGAGAAGGAAACCAGAAAGATCATGCGAGCTCTGCTGGAGGTGATCTGCACC TTGCACAAACTCAACATCGTGCACCGGGACCTGAAGCCCGAGAACATTCTCTTGGATGACAACATGAACA TCAAGCTCACAGACTTTGGCTTTTCCTGCCAGCTGGAGCCGGGAGAGAGGCTGCGAGAGGTCTGCGGGAC CCCCAGTTACCTGGCCCCTGAGATTATCGAGTGCTCCATGAATGAGGACCACCCGGGCTACGGGAAAGAG GTGGACATGTGGAGCACTGGCGTCATCATGTACACGCTGCTGGCCGGCTCCCCGCCCTTCTGGCACCGGA AGCAGATGCTGATGCTGAGGATGATCATGAGCGGCAACTACCAGTTTGGCTCGCCCGAGTGGGATGATTA CTCGGACACCGTGAAGGACCTGGTCTCCCGATTCCTGGTGGTGCAACCCCAGAACCGCTACACAGCGGAA GAGGCCTTGGCACACCCCTTCTTCCAGCAGTACTTGGTGGAGGAAGTGCGGCACTTCAGCCCCCGGGGGA AGTTCAAGGTGATCGCTCTGACCGTGCTGGCTTCAGTGCGGATCTACTACCAGTACCGCCGGGTGAAGCC TGTGACCCGGGAGATCGTCATCCGAGACCCCTATGCCCTCCGGCCTCTGCGCCGGCTCATCGACGCCTAC GCTTTCCGAATCTATGGCCACTGGGTGAAGAAGGGGCAGCAGCAGAACCGGGCAGCCCTTTTCGAGAACA CACCCAAGGCCGTGCTCCTCTCCCTGGCCGAGGAGGACTACTGA >OriGene 5' read for mutant NM_006213 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGCTCGGGACA CGGCTTCAGCCCTCTGTGGTCCCCTCTCCCCGGGGGGCTTTGGGATTCTTGTCAAGCTCCTTCAAGAGCC TGCAAGCACTTAACCAGCCACCCAGAGTTCCCTCACTGAAGATCTGAGCATGACCCGGGACGAGGCACTG CCGGACTCTCATTCTGCACAGGACTTCTATGAGAATTATGAGCCCCAAAGAGATCCTGGGCAGGGGCGTT AGCAGTGTGGTCAGGCGATGCATCCACAAGCCCACGAGCCCAGGAGTACCCCCGTGATGGGTCATCGACG TCACCGGTGGGAGGCAGCTTTCAGCCCCGGAGGAAGGTGCGGGAAGCTGCGGGAAGCCAACGCTGAGGAA GGTGGACATCCCTGGGCAGGGCTTAAGGGAACCCCAACACTATAACGGCTGAAGGACATTTATAAGCCCA CAATTTCCTCTCCCTGTGTGTTGGCCCTGTGAGAGAGAGGGGAGTTCTTGGCTTCCTTAACTAGAAGGCA CCTGATGAGAAGGGACCCGGAGGTCTATCGAGCTGCCTGAGTGATATGGCACTTGGCCAACTCAATCTGG ACGACCTGAGCCGGAAATTCTGGATACACATGACTAGCTCAAACTGCTACGCACTGACCGGAGAGCTCAA GTCCCGAACCGGTACTGCTGAATCCAGGTCCTAATAGAACGCACGGATGATTGAACTGCTAGTAGCTTGG CGTCGCCTTG >SC323335 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSAAGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGCTCGGGACACGGCT TCAGCCCTCTGTGGTCCCCTCTCCCCGGGGGGCTTTGGGATTCTTGTCAAGCTCCTTCAAGAGCCTGCAA GCACTTAACCAGCCACCCAGAGTTCCCTCACTGAAGATCTGAGCA |
| Restriction Sites | Please inquire |
| ACCN | NM_006213 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006213.2, NP_006204.1 |
| RefSeq Size | 2130 bp |
| RefSeq ORF | 2130 bp |
| Locus ID | 5260 |
| UniProt ID | Q16816 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Insulin signaling pathway |
| Gene Summary | This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208591 | PHKG1 (Myc-DDK-tagged)-Human phosphorylase kinase, gamma 1 (muscle) (PHKG1) |
CNY 3,656.00 |
|
| RC208591L1 | Lenti ORF clone of Human phosphorylase kinase, gamma 1 (muscle) (PHKG1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208591L2 | Lenti ORF clone of Human phosphorylase kinase, gamma 1 (muscle) (PHKG1), mGFP tagged |
CNY 5,890.00 |
|
| RC208591L3 | Lenti ORF clone of Human phosphorylase kinase, gamma 1 (muscle) (PHKG1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208591L4 | Lenti ORF clone of Human phosphorylase kinase, gamma 1 (muscle) (PHKG1), mGFP tagged |
CNY 5,890.00 |
|
| RG208591 | PHKG1 (tGFP-tagged) - Human phosphorylase kinase, gamma 1 (muscle) (PHKG1) |
CNY 5,256.00 |
|
| SC116261 | PHKG1 (untagged)-Human phosphorylase kinase, gamma 1 (muscle) (PHKG1) |
CNY 5,200.00 |
