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RSK1 p90 (RPS6KA1) (NM_002953) Human Untagged Clone
CAT＃: SC323333
RPS6KA1 (untagged)-Kinase deficient mutant (K94M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1
CNY 5,392.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HU-1; MAPKAPK1; MAPKAPK1A; p90Rsk; RSK; RSK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323333 sequence for NM_002953 edited (data generated by NextGen Sequencing)
ATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCATGGAGCTAGTGCCGCTGGACCCG GAGAATGGACAGACCTCAGGGGAAGAAGCTGGACTTCAGCCGTCCAAGGATGAGGGCGTC CTCAAGGAGATCTCCATCACGCACCACGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCC CATTTCGAGCTCCTCAAGGTTCTGGGCCAGGGATCCTTTGGCAAAGTCTTCCTGGTGCGG AAAGTCACCCGGCCTGACAGTGGGCACCTGTATGCTATGATGGTGCTGAAGAAGGCAACG CTGAAAGTACGTGACCGCGTCCGGACCAAGATGGAGAGAGACATCCTGGCTGATGTAAAT CACCCATTCGTGGTGAAGCTGCACTATGCCTTCCAGACCGAGGGCAAGCTCTATCTCATT CTGGACTTCCTGCGTGGTGGGGACCTCTTCACCCGGCTCTCAAAAGAGGTGATGTTCACG GAGGAGGATGTGAAGTTTTACCTGGCCGAGCTGGCTCTGGGCCTGGATCACCTGCACAGC CTGGGTATCATTTACAGAGACCTCAAGCCTGAGAACATCCTTCTGGATGAGGAGGGCCAC ATCAAACTCACTGACTTTGGCCTGAGCAAAGAGGCCATTGACCACGAGAAGAAGGCCTAT TCTTTCTGCGGGACAGTGGAGTACATGGCCCCTGAGGTCGTCAACCGCCAGGGCCACTCC CATAGTGCGGACTGGTGGTCCTATGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTG CCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACACTGATTCTGAAGGCGAAGCTAGGC ATGCCCCAGTTTCTGAGCACTGAAGCCCAGAGCCTCTTGCGGGCCCTGTTCAAGCGGAAT CCTGCCAACCGGCTCGGCTCCGGCCCTGATGGGGCAGAGGAAATCAAGCGGCATGTCTTC TACTCCACCATTGACTGGAATAAGCTATACCGTCGTGAGATCAAGCCACCCTTCAAGCCA GCAGTGGCTCAGCCTGATGACACCTTCTACTTTGACACCGAGTTCACGTCCCGCACACCC AAGGATTCCCCAGGCATCCCCCCCAGCGCTGGGGCCCATCAGCTGTTCCGGGGCTTCAGC TTCGTGGCCACCGGCCTGATGGAAGACGACGGCAAGCCTCGTGCCCCGCAGGCACCCCTG CACTCGGTGGTACAGCAACTCCATGGGAAGAACCTGGTTTTTAGTGACGGCTACGTGGTA AAGGAGACAATTGGTGTGGGCTCCTACTCTGAGTGCAAGCGCTGTGTCCACAAGGCCACC AACATGGAGTATGCTGTCAAGGTCATTGATAAGAGCAAGCGGGATCCTTCAGAAGAGATT GAGATTCTTCTGCGGTATGGCCAGCACCCCAACATCATCACTCTGAAAGATGTGTATGAT GATGGCAAACACGTGTACCTGGTGACAGAGCTGATGCGGGGTGGGGAGCTGCTGGACAAG ATCCTGCGGCAGAAGTTCTTCTCAGAGCGGGAGGCCAGCTTTGTCCTGCACACCATTGGC AAAACTGTGGAGTATCTGCACTCACAGGGGGTTGTGCACAGGGACCTGAAGCCCAGCAAC ATCCTGTATGTGGACGAGTCCGGGAATCCCGAGTGCCTGCGCATCTGTGACTTTGGTTTT GCCAAACAGCTGCGGGCTGAGAATGGGCTCCTCATGACACCTTGCTACACAGCCAACTTT GTGGCGCCTGAGGTGCTGAAGCGCCAGGGCTACGATGAAGGCTGCGACATCTGGAGCCTG GGCATTCTGCTGTACACCATGCTGGCAGGATATACTCCATTTGCCAACGGTCCCAGTGAC ACACCAGAGGAAATCCTAACCCGGATCGGCAGTGGGAAGTTTACCCTCAGTGGGGGAAAT TGGAACACAGTTTCAGAGACAGCCAAGGACCTGGTGTCCAAGATGCTACACGTGGATCCC CACCAGCGCCTCACAGCTAAGCAGGTTCTGCAGCATCCATGGGTCACCCAGAAAGACAAG CTTCCCCAAAGCCAGCTGTCCCACCAGGACCTACAGCTTGTGAAGGGAGCCATGGCTGCC ACGTACTCCGCACTCAACAGCTCCAAGCCCACCCCCCAGCTGAAGCCCATCGAGTCATCC ATCCTGGCCCAGCGGCGAGTGAGGAAGTTGCCATCCACCACCCTGTGA Clone variation with respect to NM_002953.3 51 t=>g;281 a=>t >OriGene 5' read for mutant NM_002953 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCGAAGTGCTAGTGCCGCGGCGGCGGCGGC GGACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCGGGTCGCAGAGCCAGGGACCCCA GGACCCGGGAGGCGGCGCAGCCGGGGCCGCCGGAGGAGCGCGGGTGACCTGGCGGCGGCGAGATGCCGCT CGCCCAGCTCAAGGAGCCCTGGCCGCTCATGGAGCTAGTGCCGCTGGACCCGGAGAAATGGACAGACCTC AGGGGAAGAAAGCTGGACTTTCAGCCCGTCCCAAGGATGAAGGGCCGTCCTCAGGAGATCCTCCATCACG CACCCACGTCCAAGGCTGGCTTCTGAGAAGGCTGATCCCATCCCATTTCGAACCTCCTCCAAGGTTTTGG GCCAGGGATCTTTTGGCAAAGTTCTTCTGGTGGCGAAAGTCACCCCGCCCTGACAGGGGGAACTGTAAGC CAATTGTTGGGTGCTGGAGAAGGAACGGTTGAAGATCGTTGCCGCGCTTCCGGACACAGATGGAAGGAGG ACCCTCTGCCTAGATGTAATTAACCAATCTGTGTGTAAGCTTGACTTAGCCTCTCAGACCGAGGAAGCTC TATATCATATCTGAATCCTCGTGTGTGGAACTTTACCGGTTCCAAAAGGTAGTCCCGAGGAGTGTGAGTT TACTGCGACTGGTTGCCGGTACCTGCGACTGGTTATTAGACTACGCTGAAACCCTTGGAGAGACCATTAA CCTCGTACTGCGGAGCCTTAGCAGAAGGCCAATTCTCCCAGACATTGACACCTGGCCTAGGTGT >SC323333 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ACGGATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCGAAGTGCTAGTGCCGCGGCGGCGGCGGCGG ACGGCCCAGCCGGAGCGCGAGGGGCTCGGGGGGGCGCGGCGGTTCGGGTCGCAGAGCCAGGGACCCCAGG ACCCGGGAGGCGGCGCAGCCGGGGCCGCCGGAGGAGCGCGGGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_002953 |
| Insert Size | 3670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002953.3, NP_002944.2 |
| RefSeq Size | 3199 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 6195 |
| UniProt ID | Q15418 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204874 | RPS6KA1 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 |
CNY 5,384.00 |
|
| RC204874L1 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, Myc-DDK-tagged |
CNY 7,784.00 |
|
| RC204874L2 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC204874L3 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, Myc-DDK-tagged |
CNY 7,784.00 |
|
| RC204874L4 | Lenti ORF clone of Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1, mGFP tagged |
CNY 7,784.00 |
|
| RG204874 | RPS6KA1 (tGFP-tagged) - Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 |
CNY 6,984.00 |
|
| SC118295 | RPS6KA1 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 |
CNY 5,392.00 |
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