Tomosyn (STXBP5) (NM_001127715) Human Untagged Clone
CAT#: SC323260
STXBP5 (untagged)-Human syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2
CNY 22,140.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | LGL3; LLGL3; Nbla04300 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC323260 representing NM_001127715.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGAAATTCAACATCAGGAAGGTGCTGGACGGCCTGACCGCCGGCTCGTCCTCGGCGTCGCAGCAG CAACAGCAGCAGCATCCGCCTGGGAACCGGGAGCCGGAGATCCAGGAAACGCTCCAGTCCGAGCACTTT CAGCTCTGCAAGACTGTTCGCCATGGATTTCCCTATCAACCCTCAGCCCTGGCCTTTGATCCTGTACAG AAGATCCTGGCAGTGGGAACTCAGACTGGTGCTTTAAGGCTCTTTGGTCGTCCAGGAGTAGAATGTTAT TGCCAGCATGACAGTGGAGCTGCAGTAATCCAGCTCCAGTTCCTGATTAATGAGGGAGCGCTTGTGAGT GCCTTGGCTGATGACACCTTACACTTATGGAATTTACGTCAGAAGAGGCCTGCCATACTACATTCGCTT AAATTTTGCAGAGAAAGGGTTACATTTTGCCATCTGCCTTTCCAGAGTAAGTGGCTCTATGTGGGCACT GAACGAGGTAATATACATATTGTCAATGTGGAGTCCTTCACACTCTCAGGCTACGTCATTATGTGGAAT AAAGCCATTGAACTGTCATCTAAATCTCACCCAGGACCTGTGGTCCATATAAGTGATAATCCAATGGAC GAGGGAAAGCTTTTGATTGGCTTTGAATCTGGAACAGTAGTTTTATGGGACCTCAAATCAAAGAAAGCC GACTACAGATACACATATGATGAGGCTATCCACTCTGTTGCTTGGCATCATGAAGGAAAACAATTTATT TGCAGTCATTCAGATGGCACCTTGACTATATGGAATGTAAGGTCCCCTGCTAAACCAGTACAGACAATC ACTCCACATGGAAAACAGTTAAAGGATGGGAAGAAGCCAGAACCATGCAAACCTATCCTCAAGGTGGAA TTCAAAACGACTAGATCTGGGGAGCCTTTTATTATTTTATCAGGAGGTTTGTCATATGATACTGTAGGA AGAAGACCTTGCTTAACAGTGATGCATGGGAAAAGCACTGCTGTGCTAGAAATGGACTATTCAATTGTT GATTTTCTAACGCTGTGTGAAACACCATACCCAAATGATTTTCAAGAACCATATGCTGTGGTTGTTCTT CTAGAAAAGGATTTAGTACTTATAGACCTTGCACAAAATGGATATCCTATATTTGAAAATCCCTACCCT TTGAGTATACATGAGTCCCCTGTTACATGTTGCGAATATTTTGCGGATTGTCCTGTGGACCTTATTCCT GCACTTTATTCTGTTGGAGCTAGACAGAAACGTCAAGGTTACAGCAAAAAGGAATGGCCCATCAACGGA GGTAATTGGGGCTTGGGTGCTCAAAGTTACCCAGAAATAATTATTACAGGGCATGCTGATGGGTCAGTT AAGTTCTGGGATGCTTCTGCAATAACTCTACAAGTATTATATAAGCTAAAGACATCTAAAGTATTTGAA AAGTCAAGAAATAAAGATGACAGGCCAAACACAGACATTGTAGATGAAGATCCATATGCCATTCAGATC ATCTCCTGGTGTCCAGAAAGTAGAATGCTGTGCATCGCTGGAGTTTCAGCTCATGTCATTATTTATAGA TTCAGCAAGCAGGAAGTAATCACAGAAGTCATTCCGATGCTTGAAGTTCGATTATTATATGAGATAAAT GATGTGGAAACTCCGGAGGGTGAGCAGCCACCACCTTTGCCAACACCCGTGGGAGGGTCCAACCCTCAG CCCATCCCTCCTCAGTCTCATCCATCTACCAGTAGCAGTTCATCTGATGGGCTTCGTGATAATGTACCT TGTTTAAAAGTTAAAAACTCACCACTTAAACAGTCTCCAGGTTATCAAACAGAACTAGTTATTCAGTTG GTTTGGGTGGGTGGAGAACCACCACAACAAATAACCAGCCTGGCAGTCAATTCTTCCTATGGACTGGTG GTTTTTGGCAATTGCAATGGCATTGCTATGGTTGACTACCTCCAGAAAGCAGTGCTGCTCAACCTGGGC ACTATTGAATTATATGGCTCTAATGATCCTTATCGGAGAGAACCCCGATCTCCTCGTAAATCTCGACAG CCTTCAGGAGCCGGTCTGTGTGATATTAGTGAAGGGACTGTTGTTCCAGAGGATCGCTGCAAATCTCCA ACCTCTGGTTCTTCATCACCACACAATTCAGATGATGAACAAAAAATGAATAATTTTATAGAAAAGGTG AAGACCAAAAGCAGAAAGTTTTCCAAGATGGTAGCCAATGATATAGCAAAGATGTCAAGGAAGTTAAGC TTACCTACTGACCTAAAGCCTGATTTAGATGTAAAGGATAACTCCTTTAGCCGATCACGGAGTTCAAGT GTAACAAGCATTGACAAAGAATCCCGAGAAGCGATCTCCGCTCTTCATTTCTGTGAAACGTTTACTCGA AAGACGGACTCGTCCCCTTCCCCTTGTCTATGGGTTGGAACAACGCTAGGAACAGTGCTTGTCATTGCA CTGAACCTTCCCCCAGGGGGAGAGCAAAGACTTCTTCAGCCAGTAATTGTGTCTCCAAGTGGTACTATA TTGAGGTTAAAAGGTGCAATCTTGAGAATGGCATTTCTGGATACCACAGGCTGCTTAATACCACCTGCG TATGAACCCTGGAGAGAGCACAATGTTCCTGAAGAAAAAGACGAAAAGGAGAAATTGAAAAAACGGCGG CCTGTCTCAGTATCCCCCTCCTCTTCTCAGGAAATTAGTGAAAACCAGTATGCAGTGATATGTTCTGAA AAGCAAGCAAAAGTAATCTCACTGCCAACCCAGAACTGTGCTTATAAGCAAAATATTACAGAGACCTCG TTTGTGCTTCGTGGAGATATTGTAGCATTGAGTAACAGTATCTGCCTTGCCTGTTTCTGTGCCAATGGA CATATAATGACTTTTAGTTTGCCAAGTTTAAGACCTCTGTTGGATGTGTATTACTTGCCCCTTACCAAT ATGCGGATAGCCAGAACGTTCTGCTTTACCAACAATGGACAAGCATTATACCTTGTTTCACCTACAGAA ATCCAGAGACTTACTTATAGTCAAGAGACCTGTGAAAATCTTCAGGAAATGTTGGGTGAACTCTTCACT CCTGTAGAAACACCTGAAGCACCAAACAGGGGATTCTTTAAAGGCTTATTTGGAGGTGGTGCACAATCT CTTGACAGAGAAGAACTATTTGGAGAATCGTCCTCAGGAAAGGCTTCAAGGAGCCTTGCACAGCATATT CCTGGCCCTGGTGGCATTGAAGGCGTAAAAGGGGCAGCATCTGGAGTTGTTGGTGAATTAGCACGAGCC AGGCTGGCACTAGATGAAAGAGGGCAGAAACTTGGCGATCTGGAAGAAAGAACTGCGGCCATGTTATCA AGTGCAGAGTCATTTTCTAAACATGCTCATGAGATTATGTTGAAATACAAAGATAAGAAGTGGTACCAG TTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001127715 |
Insert Size | 3456 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001127715.2 |
RefSeq Size | 9352 bp |
RefSeq ORF | 3456 bp |
Locus ID | 134957 |
UniProt ID | Q5T5C0 |
MW | 127.6 kDa |
Gene Summary | Syntaxin 1 is a component of the 7S and 20S SNARE complexes which are involved in docking and fusion of synaptic vesicles with the presynaptic plasma membrane. This gene encodes a syntaxin 1 binding protein. In rat, a similar protein dissociates syntaxin 1 from the Munc18/n-Sec1/rbSec1 complex to form a 10S complex, an intermediate which can be converted to the 7S SNARE complex. Thus this protein is thought to be involved in neurotransmitter release by stimulating SNARE complex formation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains an additional segment in the coding region, compared to variant 1, resulting in a longer isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226345 | STXBP5 (Myc-DDK-tagged)-Human syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2 |
CNY 8,016.00 |
|
RC226345L3 | Lenti ORF clone of Human syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2, Myc-DDK-tagged |
CNY 13,400.00 |
|
RC226345L4 | Lenti ORF clone of Human syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2, mGFP tagged |
CNY 13,400.00 |
|
RG226345 | STXBP5 (tGFP-tagged) - Human syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2 |
CNY 12,640.00 |
其它Tomosyn产品