Tomosyn (STXBP5) (NM_001127715) Human Untagged Clone

CAT#: SC323260

STXBP5 (untagged)-Human syntaxin binding protein 5 (tomosyn) (STXBP5), transcript variant 2



  "NM_001127715" in other vectors (4)

CNY 22,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms LGL3; LLGL3; Nbla04300
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC323260 representing NM_001127715.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGGAAATTCAACATCAGGAAGGTGCTGGACGGCCTGACCGCCGGCTCGTCCTCGGCGTCGCAGCAG
CAACAGCAGCAGCATCCGCCTGGGAACCGGGAGCCGGAGATCCAGGAAACGCTCCAGTCCGAGCACTTT
CAGCTCTGCAAGACTGTTCGCCATGGATTTCCCTATCAACCCTCAGCCCTGGCCTTTGATCCTGTACAG
AAGATCCTGGCAGTGGGAACTCAGACTGGTGCTTTAAGGCTCTTTGGTCGTCCAGGAGTAGAATGTTAT
TGCCAGCATGACAGTGGAGCTGCAGTAATCCAGCTCCAGTTCCTGATTAATGAGGGAGCGCTTGTGAGT
GCCTTGGCTGATGACACCTTACACTTATGGAATTTACGTCAGAAGAGGCCTGCCATACTACATTCGCTT
AAATTTTGCAGAGAAAGGGTTACATTTTGCCATCTGCCTTTCCAGAGTAAGTGGCTCTATGTGGGCACT
GAACGAGGTAATATACATATTGTCAATGTGGAGTCCTTCACACTCTCAGGCTACGTCATTATGTGGAAT
AAAGCCATTGAACTGTCATCTAAATCTCACCCAGGACCTGTGGTCCATATAAGTGATAATCCAATGGAC
GAGGGAAAGCTTTTGATTGGCTTTGAATCTGGAACAGTAGTTTTATGGGACCTCAAATCAAAGAAAGCC
GACTACAGATACACATATGATGAGGCTATCCACTCTGTTGCTTGGCATCATGAAGGAAAACAATTTATT
TGCAGTCATTCAGATGGCACCTTGACTATATGGAATGTAAGGTCCCCTGCTAAACCAGTACAGACAATC
ACTCCACATGGAAAACAGTTAAAGGATGGGAAGAAGCCAGAACCATGCAAACCTATCCTCAAGGTGGAA
TTCAAAACGACTAGATCTGGGGAGCCTTTTATTATTTTATCAGGAGGTTTGTCATATGATACTGTAGGA
AGAAGACCTTGCTTAACAGTGATGCATGGGAAAAGCACTGCTGTGCTAGAAATGGACTATTCAATTGTT
GATTTTCTAACGCTGTGTGAAACACCATACCCAAATGATTTTCAAGAACCATATGCTGTGGTTGTTCTT
CTAGAAAAGGATTTAGTACTTATAGACCTTGCACAAAATGGATATCCTATATTTGAAAATCCCTACCCT
TTGAGTATACATGAGTCCCCTGTTACATGTTGCGAATATTTTGCGGATTGTCCTGTGGACCTTATTCCT
GCACTTTATTCTGTTGGAGCTAGACAGAAACGTCAAGGTTACAGCAAAAAGGAATGGCCCATCAACGGA
GGTAATTGGGGCTTGGGTGCTCAAAGTTACCCAGAAATAATTATTACAGGGCATGCTGATGGGTCAGTT
AAGTTCTGGGATGCTTCTGCAATAACTCTACAAGTATTATATAAGCTAAAGACATCTAAAGTATTTGAA
AAGTCAAGAAATAAAGATGACAGGCCAAACACAGACATTGTAGATGAAGATCCATATGCCATTCAGATC
ATCTCCTGGTGTCCAGAAAGTAGAATGCTGTGCATCGCTGGAGTTTCAGCTCATGTCATTATTTATAGA
TTCAGCAAGCAGGAAGTAATCACAGAAGTCATTCCGATGCTTGAAGTTCGATTATTATATGAGATAAAT
GATGTGGAAACTCCGGAGGGTGAGCAGCCACCACCTTTGCCAACACCCGTGGGAGGGTCCAACCCTCAG
CCCATCCCTCCTCAGTCTCATCCATCTACCAGTAGCAGTTCATCTGATGGGCTTCGTGATAATGTACCT
TGTTTAAAAGTTAAAAACTCACCACTTAAACAGTCTCCAGGTTATCAAACAGAACTAGTTATTCAGTTG
GTTTGGGTGGGTGGAGAACCACCACAACAAATAACCAGCCTGGCAGTCAATTCTTCCTATGGACTGGTG
GTTTTTGGCAATTGCAATGGCATTGCTATGGTTGACTACCTCCAGAAAGCAGTGCTGCTCAACCTGGGC
ACTATTGAATTATATGGCTCTAATGATCCTTATCGGAGAGAACCCCGATCTCCTCGTAAATCTCGACAG
CCTTCAGGAGCCGGTCTGTGTGATATTAGTGAAGGGACTGTTGTTCCAGAGGATCGCTGCAAATCTCCA
ACCTCTGGTTCTTCATCACCACACAATTCAGATGATGAACAAAAAATGAATAATTTTATAGAAAAGGTG
AAGACCAAAAGCAGAAAGTTTTCCAAGATGGTAGCCAATGATATAGCAAAGATGTCAAGGAAGTTAAGC
TTACCTACTGACCTAAAGCCTGATTTAGATGTAAAGGATAACTCCTTTAGCCGATCACGGAGTTCAAGT
GTAACAAGCATTGACAAAGAATCCCGAGAAGCGATCTCCGCTCTTCATTTCTGTGAAACGTTTACTCGA
AAGACGGACTCGTCCCCTTCCCCTTGTCTATGGGTTGGAACAACGCTAGGAACAGTGCTTGTCATTGCA
CTGAACCTTCCCCCAGGGGGAGAGCAAAGACTTCTTCAGCCAGTAATTGTGTCTCCAAGTGGTACTATA
TTGAGGTTAAAAGGTGCAATCTTGAGAATGGCATTTCTGGATACCACAGGCTGCTTAATACCACCTGCG
TATGAACCCTGGAGAGAGCACAATGTTCCTGAAGAAAAAGACGAAAAGGAGAAATTGAAAAAACGGCGG
CCTGTCTCAGTATCCCCCTCCTCTTCTCAGGAAATTAGTGAAAACCAGTATGCAGTGATATGTTCTGAA
AAGCAAGCAAAAGTAATCTCACTGCCAACCCAGAACTGTGCTTATAAGCAAAATATTACAGAGACCTCG
TTTGTGCTTCGTGGAGATATTGTAGCATTGAGTAACAGTATCTGCCTTGCCTGTTTCTGTGCCAATGGA
CATATAATGACTTTTAGTTTGCCAAGTTTAAGACCTCTGTTGGATGTGTATTACTTGCCCCTTACCAAT
ATGCGGATAGCCAGAACGTTCTGCTTTACCAACAATGGACAAGCATTATACCTTGTTTCACCTACAGAA
ATCCAGAGACTTACTTATAGTCAAGAGACCTGTGAAAATCTTCAGGAAATGTTGGGTGAACTCTTCACT
CCTGTAGAAACACCTGAAGCACCAAACAGGGGATTCTTTAAAGGCTTATTTGGAGGTGGTGCACAATCT
CTTGACAGAGAAGAACTATTTGGAGAATCGTCCTCAGGAAAGGCTTCAAGGAGCCTTGCACAGCATATT
CCTGGCCCTGGTGGCATTGAAGGCGTAAAAGGGGCAGCATCTGGAGTTGTTGGTGAATTAGCACGAGCC
AGGCTGGCACTAGATGAAAGAGGGCAGAAACTTGGCGATCTGGAAGAAAGAACTGCGGCCATGTTATCA
AGTGCAGAGTCATTTTCTAAACATGCTCATGAGATTATGTTGAAATACAAAGATAAGAAGTGGTACCAG
TTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001127715
Insert Size 3456 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001127715.2
RefSeq Size 9352 bp
RefSeq ORF 3456 bp
Locus ID 134957
UniProt ID Q5T5C0
MW 127.6 kDa
Gene Summary Syntaxin 1 is a component of the 7S and 20S SNARE complexes which are involved in docking and fusion of synaptic vesicles with the presynaptic plasma membrane. This gene encodes a syntaxin 1 binding protein. In rat, a similar protein dissociates syntaxin 1 from the Munc18/n-Sec1/rbSec1 complex to form a 10S complex, an intermediate which can be converted to the 7S SNARE complex. Thus this protein is thought to be involved in neurotransmitter release by stimulating SNARE complex formation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) contains an additional segment in the coding region, compared to variant 1, resulting in a longer isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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