USP8 (NM_001128610) Human Untagged Clone

CAT#: SC323258

USP8 (untagged)-Human ubiquitin specific peptidase 8 (USP8), transcript variant 2



  "NM_001128610" in other vectors (4)

CNY 10,450.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-USP8 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HumORF8; PITA4; SPG59; UBPY
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001128610 edited
GCGAATTCGCACGAGGGTCCTGGTAGCCAACGCTAATTCTCCCTCGAGTTCTTGGGAGAT
GGGCATTTGGCGAGAAGGCTGGCGTTAGTGAAGCGCGCCCGGCGTCACGGAAAGAAGCAC
TTGTAAGGAAATATAGCATCCATTGTGAAAGTGGAAAAGTAAAGATAATTCATCATGCCT
GCTGTGGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTTAATAAG
AAGACAGAAGTTAAACCAGAGAAAATAAGCACTAAGAGTTATGTGCACAGTGCCCTGAAG
ATCTTTAAGACAGCAGAAGAATGCAGATTAGATCGTGATGAGGAAAGGGCCTATGTACTA
TATATGAAATACGTGACTGTTTATAATCTTATCAAAAAAAGACCTGATTTCAAGCAACAG
CAGGATTATTTCCATTCAATACTTGGACCTGGAAACATCAAAAAAGCTGTCGAAGAAGCT
GAAAGACTCTCTGAAAGCCTTAAATTAAGATATGAAGAAGCTGAAGTCCGGAAAAAACTT
GAGGAAAAAGACAGGCAGGAGGAAGCACAGCGGCTACAACAAAAAAGGCAGGAAACAGGA
AGAGAGGATGGTGGCACATTGGCTAAAGGCTCTTTGGAGAATGTTTTGGATTCCAAAGAC
AAAACCCAAAAGAGCAATGGTGAAAAGAATGAAAAATGTGAGACCAAAGAGAAAGGAGCA
ATCACAGCAAAGGAACTATACACAATGATGACGGATAAAAACATCAGCTTGATTATAATG
GATGCTCGAAGAATGCAGGATTATCAGGATTCCTGTATTTTACATTCTCTCAGTGTTCCT
GAAGAAGCCATCAGTCCAGGAGTCACTGCTAGTTGGATTGAAGCACACCTGCCAGATGAT
TCTAAAGACACATGGAAGAAGAGGGGGAATGTGGAGTATGTGGTACTTCTTGACTGGTTT
AGTTCTGCCAAAGATTTACAGATTGGAACAACTCTCCGGAGTCTGAAAGATGCACTTTTC
AAGTGGGAAAGTAAAACTGTCCTGCGCAATGAGCCTTTGGTTTTAGAGGGAGGCTATGAA
AACTGGCTCCTTTGTTATCCCCAGTATACAACAAATGCTAAGGTCACTCCACCCCCACGA
CGCCAGAATGAAGAGGTGTCTATCTCATTGGATTTTACTTATCCCTCATTGGAAGAATCA
ATTCCTTCTAAACCTGCTGCCCAGACGCCACCTGCATCTATAGAAGTAGATGAAAATATA
GAATTGATAAGTGGTCAAAATGAGAGAATGGGACCACTGAATATATCAACTCCAGTTGAA
CCAGTTGCTGCTTCTAAATCTGATGTTTCACCCATAATTCAGCCAGTGCCTAGTATAAAG
AATGTTCCACAGATTGATCGTACTAAAAAACCAGCAGTCAAATTGCCTGAAGAGCATAGA
ATAAAATCTGAAAGTACAAACCATGAGCAACAATCTCCTCAGAGTGGAAAAGTTATTCCT
GATCGTTCCACCAAGCCAGTAGTTTTTTCTCCAACTCTCATGTTAACAGATGAAGAAAAG
GCTCGTATTCATGCAGAAACTGCTCTTCTAATGGAAAAAAACAAACAAGAAAAAGAACTT
CGGGAAAGGCAGCAAGAGGAACAGAAAGAGAAACTGAGGAAGGAAGAACAAGAACAAAAA
GCCAAAAAGAAACAAGAAGCTGAAGAAAATGAAATTACAGAGAAGCAACAAAAAGCAAAA
GAAGAAATGGAGAAGAAAGAAAGTGAACAGGCCAAGAAAGAAGATAAAGAAACCTCAGCA
AAGAGGGGCAAAGAAATAACAGGAGTAAAAAGACAAAGTAAAAGTGAACATGAAACTTCT
GATGCCAAGAAATCTGTAGAAGATAGGGGGAAAAGGTGTCCAACCCCAGAAATACAGAAA
AAGTCAACAGGAGATGTGCCCCATACATCTGTGACAGGGGATTCAGGTTCAGGCAAGCCA
TTTAAGATTAAAGGACAACCAGAAAGTGGAATTCTAAGGACAGGAACTTTTAGAGAGGAT
ACAGACGATACCGAAAGAAATAAAGCTCAACGAGAACCTTTGACAAGAGCACGAAGTGAA
GAAATGGGGAGGATCGTACCAGGACTGCCTTCAGGCTGGGCCAAGTTTCTTGACCCAATC
ACTGGAACCTTTCGTTATTATCATTCACCCACCAACACTGTTCATATGTACCCACCGGAA
ATGGCTCCTTCATCTGCACCTCCTTCCACCCCTCCAACTCATAAAGCCAAGCCACAGATT
CCTGCTGAGCGGGATAGGGAACCTTCCAAACTGAAGCGCTCCTACTCCTCCCCAGATATA
ACCCAGGCTATTCAAGAGGAAGAGAAGAGGAAGCCAACAGTAACTCCAACAGTTAATCGG
GAAAACAAGCCAACATGTTATCCTAAAGCTGAGATCTCAAGGCTTTCTGCTTCTCAGATT
CGGAACCTCAATCCTGTTTTTGGAGGTTCTGGACCAGCTCTTACTGGACTTCGTAACTTA
GGAAATACTTGTTATATGAACTCAATATTGCAGTGCCTATGTAACGCTCCACATTTGGCT
GATTATTTCAACCGAAACTGTTATCAGGATGATATTAACAGGTCAAATTTGTTGGGGCAT
AAAGGTGAAGTGGCAGAAGAATTTGGTATAATCATGAAAGCCCTGTGGACAGGACAGTAT
AGATATATCAGTCCAAAGGACTTTAAAATCACCATTGGGAAGATCAATGACCAGTTTGCA
GGATACAGTCAGCAAGATTCACAAGAATTGCTTCTGTTCCTAATGGATGGTCTCCATGAA
GATCTAAATAAAGCTGATAATCGGAAGAGATATAAAGAAGAAAATAATGATCATCTCGAT
GACTTTAAAGCTGCAGAACATGCCTGGCAGAAACACAAGCAGCTCAATGAGTCTATTATT
GTTGCACTTTTTCAGGGTCAATTCAAATCTACAGTACAGTGCCTCACATGTCACAAAAAG
TCTAGGACATTTGAGGCCTTCATGTATTTGTCTCTACCACTAGCATCCACAAGTAAATGT
ACATTACAGGATTGCCTTAGATTATTTTCCAAAGAAGAAAAACTCACAGATAACAACAGA
TTTTACTGCAGTCATTGCAGAGCTCGACGGGATTCTCTAAAAAAGATAGAAATCTGGAAG
TTACCACCTGTGCTTTTAGTGCATCTGAAACGTTTTTCCTACGATGGCAGGTGGAAACAA
AAATTACAGACATCTGTGGACTTCCCGTTAGAAAATCTTGACTTGTCACAGTATGTTATT
GGTCCAAAGAACAATTTGAAGAAATATAATTTGTTTTCTGTTTCAAATCACTACGGTGGG
CTGGATGGAGGCCACTACACAGCCTATTGTAAAAATGCAGCAAGACAACGGTGGTTTAAG
TTTGATGATCATGAAGTTTCTGATATCTCCGTTTCTTCTGTGAAATCTTCAGCAGCTTAT
ATCCTCTTTTATACTTCATTGGGACCACGAGTAACTGATGTAGCCACATAAGGAGACATA
GGTTATAAACTAGTTATCTTTTAAAAGGCTCAGCAACACAACTCTTGAAATGCTTATCAG
GATAATGGTAGCTATAGCTGGCCATTTAGAGGAATTCTAGGACAGTGGGAGCTGTGTTAC
TAGCACTATATAATTCCGGTCAGTGCTGACAAATAACATTTAACAAGTATTGCAGTAATC
ATCACTTACAGGTACCATTTATTTCAAAACAACTTTTTTAGTCTGCTCCAAAGTTAAAAT
AATTAACTAGCTAAGCATTATTATTCGACTGGTCTAAAAACTATTGTTATCTTTTTTTTT
CCTTTTCACTGTTATGGCCTTTTCACATTTCTAAATCCCATCTTGATATACTATGAATAC
TCTAGAATGATGTAAAGC
Restriction Sites Please inquire     
ACCN NM_001128610
Insert Size 3900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001128610.1.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001128610.1, NP_001122082.1
RefSeq Size 5704 bp
RefSeq ORF 3357 bp
Locus ID 9101
UniProt ID P40818
Protein Families Druggable Genome, Protease
Protein Pathways Endocytosis
Gene Summary This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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