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Ataxin 1 (ATXN1) (NM_001128164) Human Untagged Clone
CAT＃: SC323209
ATXN1 (untagged)-Human ataxin 1 (ATXN1), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATX1; D6S504E; SCA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC323209 representing NM_001128164.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAAGAAGCGCGAGATCCCCGCCACCAGC CGGTCCTCCGAGGAGAAGGCCCCTACCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTC CCGGGCAACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGACCTCGGTGGAGCTT GGTTTACAACAGGGAATAGGTTTACACAAAGCATTGTCCACAGGGCTGGACTACTCCCCGCCCAGCGCT CCCAGGTCTGTCCCCGTGGCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGTG TCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGTCCTCCCAATACAGTGGAACC TATGCCAGCTTCATCCCATCACAGCTGATCCCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCG GCCGCAGGGGCCACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGGCCAACATG GGCAGTCTGAGCCAGACGCCGGGACACAAGGCTGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG CAGCATCAGCATCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCT CCGGGGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTCCACATTTCCAGTTCT CCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGCCATCCCCGTCCACCTCCACCCCCACCAGACGATG ATCCCACACACGCTCACCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGCCAC TTTGTCCCTCGGGAGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCAGCAGGCCATCCAGGCCAAGGAG GTCCTGAACGGTGAGATGGAGAAGAGCCGGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGC AAGGCAGGCGGCAAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCCGAGCCCCTCA GACTACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTGTGATGGTCCTGCCCAACAGCAACACGCCC GCAGCTGACCTGGAGGTGCAACAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGT GGCCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCACCCCACACGGTCATTCAGACCACA CACAGTGCTTCAGAGCCACTCCCGGTGGGACTGCCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCT GTCATCGGCTACCTGAGCGGCCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACCTGGTG ATCCCCGGCACACAGCCCCTGCTCATCCCGGTCGGCAGCACTGACATGGAAGCGTCGGGGGCAGCCCCG GCCATAGTCACGTCATCCCCCCAGTTTGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAG AGCGAGAACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTGCAGGCCCAGATC CACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGCGGCTCCCCCTACGCTGCCTCCCTACTTCATG AAAGGCTCCATCATCCAGTTGGCCAACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTC ATCCAGAGTGCAGAGATAAGCAACGACCTGAAGATCGACTCCAGCACCGTAGAGAGGATTGAAGACAGC CATAGCCCGGGCGTGGCCGTGATACAGTTCGCCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTT TTGGTAGAGTATCCTTTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAACCAGCCAG CTCTTTGATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCTGCATCTCGCTTACCCTCAAGAACCTG AAGAACGGCTCTGTTAAAAAGGGCCAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCC GACGGCCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGAATCAACCAGGGGAGTGCCCAG ATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAGAAAATGGGATTGCCTGCAGCGCCCTTCCTCACC AAAATAGAACCCAGCAAGCCCGCGGCAACGAGGAAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAACTG GAGAAGTCAGAAGACGAACCACCTTTGACTCTTCCTAAGCCTTCTCTAATTCCTCAGGAGGTTAAGATT TGCATTGAAGGCCGGTCTAATGTAGGCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001128164 |
| Insert Size | 2448 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128164.1 |
| RefSeq Size | 10587 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 6310 |
| UniProt ID | P54253 |
| MW | 86.9 kDa |
| Gene Summary | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). Alternative splicing results in multiple transcript variants, with one variant encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to the use of overlapping alternate reading frames. [provided by RefSeq, Nov 2017] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. This variant encodes the same protein represented by the longer protein encoded by variant 1, ATXN1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226185 | ATXN1 (Myc-DDK-tagged)-Human ataxin 1 (ATXN1), transcript variant 2 |
CNY 5,968.00 |
|
| RC226185L3 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226185L4 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG226185 | ATXN1 (tGFP-tagged) - Human ataxin 1 (ATXN1), transcript variant 2 |
CNY 4,370.00 |
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