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FLIP (CFLAR) (NM_001127184) Human Untagged Clone
CAT＃: SC322904
CFLAR (untagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3
CNY 3,840.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; cFLIP; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC322904 representing NM_001127184.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTGCTGAAGTCATCCATCAGGTTGAAGAAGCACTTGATACAGATGAGAAGGAGATGCTGCTCTTT TTGTGCCGGGATGTTGCTATAGATGTGGTTCCACCTAATGTCAGGGACCTTCTGGATATTTTACGGGAA AGAGGTAAGCTGTCTGTCGGGGACTTGGCTGAACTGCTCTACAGAGTGAGGCGATTTGACCTGCTCAAA CGTATCTTGAAGATGGACAGAAAAGCTGTGGAGACCCACCTGCTCAGGAACCCTCACCTTGTTTCGGAC TATAGAGTGCTGATGGCAGAGATTGGTGAGGATTTGGATAAATCTGATGTGTCCTCATTAATTTTCCTC ATGAAGGATTACATGGGCCGAGGCAAGATAAGCAAGGAGAAGAGTTTCTTGGACCTTGTGGTTGAGTTG GAGAAACTAAATCTGGTTGCCCCAGATCAACTGGATTTATTAGAAAAATGCCTAAAGAACATCCACAGA ATAGACCTGAAGACAAAAATCCAGAAGTACAAGCAGTCTGTTCAAGGAGCAGGGACAAGTTACAGGAAT GTTCTCCAAGCAGCAATCCAAAAGAGTCTCAAGGATCCTTCAAATAACTTCAGGATGATAACACCCTAT GCCCATTGTCCTGATCTGAAAATTCTTGGAAATTGTTCCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001127184 |
| Insert Size | 666 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001127184.2 |
| RefSeq Size | 1299 bp |
| RefSeq ORF | 666 bp |
| Locus ID | 8837 |
| UniProt ID | O15519 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Apoptosis |
| MW | 25.4 kDa |
| Gene Summary | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (3) represents use of an alternate coding exon that results in a shorter protein isoform (2) with a distinct C-terminus. Variants 3, 12, and 13 all encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225270 | CFLAR (Myc-DDK-tagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3 |
CNY 3,600.00 |
|
| RC225270L1 | Lenti-ORF clone of CFLAR (Myc-DDK-tagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3 |
CNY 6,000.00 |
|
| RC225270L2 | Lenti-ORF clone of CFLAR (mGFP-tagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3 |
CNY 5,890.00 |
|
| RC225270L3 | Lenti-ORF clone of CFLAR (Myc-DDK-tagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3 |
CNY 5,890.00 |
|
| RC225270L4 | Lenti-ORF clone of CFLAR (mGFP-tagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3 |
CNY 5,890.00 |
|
| RG225270 | CFLAR (tGFP-tagged) - Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 3 |
CNY 5,200.00 |
