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PPCS (NM_001077447) Human Untagged Clone
CAT＃: SC322766
PPCS (untagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 2
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMD2C |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322766
GGCGCCGGCCGCTGCGCTGCAGATGGCGGAAATGGATCCGGTAGCCGAGTTCCCCCAGCC
TCCCGGTGCTGCGCGCTGGGCTGAGGTTATGGCTCGCTTCGCGGCCAGGCTGGGCGCGCA GGGCCGGCGGGTGGTGTTGGTTACGTCAGGCGGCACCAAGGTCCCACTGGAAGCGCGGCC GGTGCGCTTCCTGGACAACTTCAGCAGCGGGCGGCGCGGTGCAACCTCGGCCGAGGCCTT CCTAGCCGCCGGCTACGGGGTCCTGTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGC CCACCGCTTCCCACCCCAGACTTGGCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTC GGGCTTGCTGAGCCTGGAGGCCGAGGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAG GAGCTACCAGGAGGCTGCGGCTGCAGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGC GGACTATTTGCATCTGTTGCAGGCTGCGGCCCAGGCACTCAATCCGCTAGGCCCTTCTGC GATGTTTTACCTGGCTGCGGCTGTGTCAGATTTCTATGTTCCTGTCTCTGAAATGCCTGA ACACAAGATCCAGTCATCTGGGGGCCCACTGCAGATAACAATGAAGATGGTGCCAAAACT GCTTTCTCCTTTGGTTAAAGATTGGGCTCCCAAAGCATTTATAATTTCCTTTAAGTTGGA GACTGACCCCGCCATTGTAATTAATCGAGCTCGGAAGGCTTTGGAAATTTATCAGCATCA AGTGGTGGTGGCTAATATCCTTGAGTCACGACAGTCCTTTGTGTTTATTGTAACCAAAGA CTCGGAAACCAAGTTATTGCTATCAGAGGAAGAAATAGAAAAAGGCGTAGAGATAGAAGA GAAGATAGTGGATAATCTTCAGTCTCGACACACAGCTTTTATAGGTGACAGAAACTGAAG TAAAAAGCCCTTATAGGATCAAAAATTGTTCAGGGCTCTTAGAGATGGTGAAAACTACAA AAAAAACCATGGCTTTCATATGGACAGAGAAAATGAAAGAAAGGGAAAAGGCAGTGGTGT GTAGGCAAATATGGTTTGGCATTTGTCTTTTAATGACACCTGATATGATGTCATTTTGAT TTTGAAATTGAACACTAGAACTGTTAATCACCTTTAAAAAGAAGAGCTTATTGGGAATTA TATATTCCTTAAAATATACATGGGGGCCTGAATGTCAGCCATCTTTATACTATAGAAAAA GGATTATGGATGCATGAATGGTCATGCTTTGGAGATCAAATATTGGTTGAATGCCTATGT ATGTCAGGCCCTGTGCTGAGCCATGAGGATTAAAAAGATGAATAAACATATCTTGTTTAG GAAATGGATGTATAAAAAAATCAAGTGCAATAAAGTGTGTGTCCAAAAGCTGACACAATG GAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001077447 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001077447.1, NP_001070915.1 |
| RefSeq Size | 1044 bp |
| RefSeq ORF | 417 bp |
| Locus ID | 79717 |
| UniProt ID | Q9HAB8 |
| Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
| Gene Summary | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 5, 6, and 7 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210740 | PPCS (Myc-DDK-tagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 2 |
CNY 2,400.00 |
|
| RC210740L3 | Lenti ORF clone of Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210740L4 | Lenti ORF clone of Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG210740 | PPCS (tGFP-tagged) - Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 2 |
CNY 4,370.00 |
