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DARS1 (NM_001349) Human Untagged Clone
CAT＃: SC321548
DARS (untagged)-Human aspartyl-tRNA synthetase (DARS)
CNY 3,736.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | aspRS; DARS; HBSL |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001349.2
CGGAAGCCTGCGAGGGAGGGTGGTGTCCACTGCCCAGTTCCGTGTCCCGATGCCCAGCGC
CAGCGCCAGCCGCAAGAGTCAGGAGAAGCCGCGGGAGATCATGGACGCGGCGGAAGATTA TGCTAAAGAGAGATATGGAATATCTTCAATGATACAATCACAAGAAAAACCAGATCGAGT TTTGGTTCGGGTTAGAGACTTGACAATACAAAAAGCTGATGAAGTTGTTTGGGTACGTGC AAGAGTTCATACAAGCAGAGCTAAAGGGAAACAGTGCTTCTTAGTCCTACGTCAGCAGCA GTTTAATGTCCAGGCTCTTGTGGCGGTGGGAGACCATGCAAGCAAGCAGATGGTTAAATT TGCTGCCAACATCAACAAAGAGAGCATTGTGGATGTAGAAGGTGTTGTGAGAAAAGTGAA TCAGAAAATTGGAAGCTGTACACAGCAAGACGTTGAGTTACATGTTCAGAAGATTTATGT GATCAGTTTGGCTGAACCCCGTCTGCCCCTGCAGCTGGATGATGCTGTTCGGCCTGAGGC AGAAGGAGAAGAGGAAGGAAGAGCTACTGTTAACCAGGATACAAGATTAGACAACAGAGT CATTGATCTTAGGACATCAACTAGTCAGGCAGTCTTCCGTCTCCAGTCTGGCATCTGCCA TCTCTTCCGAGAAACTTTAATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATTAT TTCAGCTGCCAGTGAAGGAGGAGCCAATGTTTTTACTGTGTCATATTTTAAAAATAATGC ATACCTGGCTCAGTCCCCACAGCTATATAAGCAAATGTGCATTTGTGCTGATTTTGAGAA GGTTTTCTCTATTGGACCAGTATTCAGAGCGGAAGACTCTAATACCCATAGACATCTAAC TGAGTTTGTTGGTTTGGACATTGAAATGGCTTTTAATTACCATTACCACGAAGTTATGGA AGAAATTGCTGACACCATGGTACAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGA AATTCAAACAGTGAATAAACAGTTCCCATGTGAGCCATTCAAATTTTTGGAGCCAACTCT AAGACTAGAATATTGTGAAGCATTGGCTATGCTTAGGGAAGCTGGAGTCGAAATGGGAGA TGAAGACGATCTGAGCACACCAAATGAAAAGCTGTTGGGTCATTTGGTAAAGGAAAAGTA TGATACAGATTTTTATATTCTTGATAAATATCCATTGGCTGTAAGACCTTTCTATACCAT GCCTGACCCAAGAAATCCCAAACAGTCCAACTCTTACGATATGTTCATGAGAGGAGAAGA AATATTGTCAGGAGCTCAAAGAATACATGATCCTCAACTGCTAACAGAGAGAGCTTTACA TCATGGAATTGATTTGGAGAAAATTAAGGCTTACATTGATTCCTTCCGCTTTGGAGCCCC TCCTCATGCTGGTGGAGGCATTGGATTGGAACGAGTTACTATGCTGTTTCTGGGATTGCA TAATGTTCGTCAGACCTCCATGTTCCCTCGTGATCCCAAACGACTCACTCCTTAAATTCA CACTTTGCCACTTAACTCCAGTGTGGATGACAGAGCGAGACCCTGCCTCAAAAAAAAAAA AAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001349 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001349.2, NP_001340.2 |
| RefSeq Size | 2322 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 1615 |
| UniProt ID | P14868 |
| Domains | tRNA-synt_2, tRNA_anti |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| Gene Summary | This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200391 | DARS (Myc-DDK-tagged)-Human aspartyl-tRNA synthetase (DARS) |
CNY 3,728.00 |
|
| RC200391L1 | Lenti ORF clone of Human aspartyl-tRNA synthetase (DARS), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC200391L2 | Lenti ORF clone of Human aspartyl-tRNA synthetase (DARS), mGFP tagged |
CNY 5,890.00 |
|
| RC200391L3 | Lenti ORF clone of Human aspartyl-tRNA synthetase (DARS), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200391L4 | Lenti ORF clone of Human aspartyl-tRNA synthetase (DARS), mGFP tagged |
CNY 5,890.00 |
|
| RG200391 | DARS (tGFP-tagged) - Human aspartyl-tRNA synthetase (DARS) |
CNY 4,370.00 |
