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Carbonic Anhydrase III (CA3) (NM_005181) Human Untagged Clone
CAT＃: SC321507
CA3 (untagged)-Human carbonic anhydrase III, muscle specific (CA3)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAIII; Car3 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_005181.2
GAGAAAGCAGGAGCCGTCCAGCACGGAGGAAGGCGACCATGGCCAAGGAGTGGGGCTACG
CCAGTCACAACGGTCCTGACCACTGGCATGAACTTTTCCCAAATGCCAAGGGGGAAAACC AGTCGCCCATTGAGCTGCATACTAAAGACATCAGGCATGACCCTTCTCTGCAGCCATGGT CTGTGTCTTATGATGGTGGCTCTGCCAAGACCATCCTGAATAATGGGAAGACCTGCCGAG TTGTATTTGATGATACTTATGATAGGTCAATGCTGAGAGGGGGTCCTCTCCCTGGACCCT ACCGACTTCGCCAGTTTCATCTTCACTGGGGCTCTTCGGATGATCATGGCTCTGAGCACA CCGTGGATGGAGTCAAGTATGCAGCGGAGCTTCATTTGGTTCACTGGAACCCGAAGTATA ACACTTTTAAAGAAGCCCTGAAGCAGCGCGATGGGATCGCTGTGATTGGCATTTTTCTGA AGATAGGACATGAGAATGGCGAGTTCCAGATTTTCCTTGATGCATTGGACAAGATTAAGA CAAAGGGCAAGGAGGCGCCCTTCACAAAGTTTGACCCATCCTGCCTGTTCCCGGCATGCC GGGACTACTGGACCTACCAGGGCTCATTCACCACGCCGCCCTGCGAGGAATGCATTGTGT GGCTGCTGCTGAAGGAGCCCATGACCGTGAGCTCTGACCAGATGGCCAAGCTGCGGAGCC TCCTCTCCAGTGCTGAGAACGAGCCCCCAGTGCCTCTTGTGAGCAACTGGCGACCTCCAC AGCCTATCAATAACAGGGTGGTGAGAGCTTCCTTCAAATGAGGCTGCTGGATCTTGCCCT CTTCAGGAAAGGAAACCTACCATTGGAGAGCTTGGTTCCTTGCCTCCTTCTGGTGCTCTT ACTCCAAGTCTATTTCATTTTTCCACACTGAGCAATGAATGTGAGAGATGTGGTCACCAA GATCTAAGTTACTTGTTGAAAGAAAGTTACTTTCGACAAGATCTAATATGAAAGCATAGA TTTCACATTTGATCTCTGTAATAATCATCTTTCCTATAAAAGTAGCATTTTTGGTAAAGT TTCAAAGAAGAAGAAACAGAGATGGAAGAGTAAAGATATTTTTAAAATGGCTAGCTATTG GGCACCAGTTTTTCTGTTATCTAAAATTTCACACAACTTCATTGTTTTTATTTTTATATT ATGAGTTGTCCATCTTAAAGAAATATGAGTAATTCTACATGTAGTAGAGGTGTATGAAGA TCATATAACAATTAAACATAAGCCAGAAATTAAAATGACTATAGACAGCAAGAATTGAGC TAATAATATGTTTTAACTCTTAACACCAGCAAGAAGTCAGTCATTTATTGAAGTTTTAGC TACTAAGATTACTTGGTTTTGATTACCAGTGAAAAGAAAACACAATACAATCAGGAGTTT TCAAATTTTTGATTCAGTATTTGAATTTCTTCTTCATAAATGTAGTTGAATTTATCCTAG TATTTTTCTTTACCTGAAGTAGGGCCATTTATTTTTAATTTCACTACATTTTTCTTTGCA TGATTATTAAAATAAAAACTGCCTCTGTTGTGTTTCTCACTGGAGGCTGGAATGAATGAT CACTAGAACACAAAAGAGTGAATGATGACACTTGAAGTCAAAGCAGTTGTACTGATCACC AGAACCAATAAAGACATAAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_005181 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005181.2, NP_005172.1 |
| RefSeq Size | 2357 bp |
| RefSeq ORF | 783 bp |
| Locus ID | 761 |
| UniProt ID | P07451 |
| Domains | carb_anhydrase |
| Protein Families | Druggable Genome |
| Protein Pathways | Nitrogen metabolism |
| Gene Summary | Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201066 | CA3 (Myc-DDK-tagged)-Human carbonic anhydrase III, muscle specific (CA3) |
CNY 2,400.00 |
|
| RC201066L3 | Lenti ORF clone of Human carbonic anhydrase III, muscle specific (CA3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201066L4 | Lenti ORF clone of Human carbonic anhydrase III, muscle specific (CA3), mGFP tagged |
CNY 5,890.00 |
|
| RG201066 | CA3 (tGFP-tagged) - Human carbonic anhydrase III, muscle specific (CA3) |
CNY 4,000.00 |
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