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DHFR (NM_000791) Human Untagged Clone
CAT＃: SC321334
DHFR (untagged)-Human dihydrofolate reductase (DHFR)
CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DHFRP1; DYR |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000791.3
CTTGACCGCGCGTTCTGCTGTAACGAGCGGGCTCGGAGGTCCTCCCGCTGCTGTCATGGT
TGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGA CCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACAACCTC TTCAGTAGAAGGTAAACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCC TGAGAAGAATCGACCTTTAAAGGGTAGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGA ACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTCTAGATGATGCCTTAAAACTTACTGA ACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGGTGGCAGTTCTGTTTA TAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATGCAAGA CTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGA ATACCCAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGT ATATGAGAAGAATGATTAATATGAAGGTGTTTTCTAGTTTAAGTTGTTCCCCCTCCCTCT GAAAAAAGTATGTATTTTTACATTAGAAAAGGTTTTTTGTTGACTTTAGATCTATAATTA TTTCTAAGCAACTAGTTTTTATTCCCCACTACTCTTGTCTCTATCAGATACCATTTATGA GACATTCTTGCTATAACTAAGTGCTTCTCCAAGACCCCAACTGAGTCCCCAGCACCTGCT ACAGTGAGCTGCCATTCCACACCCATCACATGTGGCACTCTTGCCAGTCCTTGACATTGT CGGGCTTTTCACATGTTGGTAATATTTATTAAAGATGAAGATCCACATACCCTTCAACTG AGCAGTTTCACTAGTGGAAATACCAAAAGCTTCCTACGTGTATATCCAGAGGTTTGTAGA TAAATGTTGCCACCTTGTTTGTAACAGTGAAAAATTGAAAACAACCTGGAAGTCCAGTGA TGGGAAAATGAGTATGTTTCTGTCTTAGATTGGGGAACCCAAAGCAGATTGCAAGACTGA AATTTCAGTGAAAGCAGTGTATTTGCTAGGTCATACCAGAAATCATCAATTGAGGTACGG AGAAACTGAACTGAGAAGGTAAGAAAAGCAATTTAAAGTCAGCGAGCAGGTTCTCATTGA TAACAAGCTCCATACTGCTGAGATACAGGGAAATGGAGGGGGGAAAGCTGGAGTATTGAT CCCGCCCCCCTCCTTGGTTGTCAGCTCCCTGTCCTGTGTGTGGGCGGAACATAGTCCAGC TGCTCTATAGCAAGTCTCAGGTGTTTGCAGTAAGAAGCTGCTGGCATGCACGGGAACAGT GAATGCCAAACACTTAAAGCAATTCGATGTTTAAGTATGTAAGTTCTTTTTTTTTTAGAC AGCGTTTCGCTCTTGTTGCCCAGGCTGGCATGCAGTGGTGTGACCTCGGCTTACTGCAAC CTCCGCCTTCCCAGATTCAAGCGATTCTCCTGCCTCAGGCTCCCAAGTAGCTAGGACCAG GTGCGCGCCACCACGCCCGGCTAATTTTTGTATTTTGTATTTTTAGTAGAGATGGGGTTT CACCATGTTGGTCAGGCTGGTCTCGAACTCGTGACCGCAAGCGATTCACCCACCTCAGCC TCCCAAAGTGCTGGGATTACCGGCTTGAGCCACCACACCCGGCACATCTTCATTCTTTTT ATGTAGTAAAAAGTATAAGGCCACACATGGTTTATTTGAAGTATTTTATAATTTAAAAAA ATACAGAAGCAGGAAAACCAATTATAAGTTCAAGTGAGGGATGATGGTTGCTTGAACCAA AGGGTTGCATGTAGTAAGAAATTGTGATTTAAGATATATTTTAAAGTTATAAGTAGCAGG ATATTCTGATGGAGTTTGACTTTGGTTTTGGGCCCAGGGAGTTTCAGATGCCTTTGAGAA ATGAATGAAGTAGAGAGAAAATAAAAGAAAAACCAGCCAGGCACAGTGGCTCACGCCTGT AATCCCAGCGCTTTGGGAGGCTAAGGCAGGCAGATCACTTGAGACCAGCTTGGGCAACAT GGCAAAGCCCCATCTCTACAAAAAACACAAAAATTAGCTGGGCATTGTGGCGCACACCTG TATTCCCATCTAGTCAGGAAGCTGAGATGGAAGAATTAATTGAGCCCACGAGTTCAAGGC TGCAGTGAGTCGTGATTGTGCCACTGCACTCCAGCCGGGGTGACAGAAGAGACCTTGTCT CGAAAAGGAATCTGAAAACAATGGAACCATGCCTTCATAATTCTAGAAAGTTATTTTCAA CTGATAAATCTATATTCACCCAAATAATCAAGGGTGAAGGTAAAATAATACATTTTTAGA CAAGCAAAGACTCAGGGGTTACCTCCATGTGCCCTTTTTAGGGAAGCTGTTGGAGAAAAT ACTCCAGCAAAATGAAGGAGTACACAAACCAGAGAATGACATGAATCCAGCAAATAGGAT CCAACACAGGCAATATTCCAGCTATGGAGCTAGCTTTAAAAAGGAACAGTAAAAATATTA ATCGGTTAGCTGGGTGGAATGGCCCATGCCTGTAGTCCCAGCTACTCAGGAGGCTCAGCA GCAGGACGACTTGAGCCCAAGAGTTCCAGACCAGCCTGGCCACCTTAGTGAGATCCCTTC TCTTAAAAATAATAACTTATTGCCAGATTTGGGGCATTTGGAAAGAAGTTCATTGAAGAT AAAGCAAAAGTAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000791 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000791.3, NP_000782.1 |
| RefSeq Size | 3932 bp |
| RefSeq ORF | 564 bp |
| Locus ID | 1719 |
| UniProt ID | P00374 |
| Domains | DiHfolate_red |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Folate biosynthesis, Metabolic pathways, One carbon pool by folate |
| Gene Summary | Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Disparate Response to Methotrexate in Stem versus Non-Stem Cells
,null,
Stem cell reviews
,PubMed ID 26815725
[DHFR]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200089 | DHFR (Myc-DDK-tagged)-Human dihydrofolate reductase (DHFR) |
CNY 2,400.00 |
|
| RC200089L1 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200089L2 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged |
CNY 4,800.00 |
|
| RC200089L3 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200089L4 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged |
CNY 5,890.00 |
|
| RG200089 | DHFR (tGFP-tagged) - Human dihydrofolate reductase (DHFR) |
CNY 4,000.00 |
