DPAGT1 (NM_001382) Human Untagged Clone
CAT#: SC320266
DPAGT1 (untagged)-Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1)
CNY 3,656.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ALG7; CDG-Ij; CDG1J; CMS13; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_001382.2
GGTGTCCCGACGGCGGCTCAAGTCAGAGTTGCTGGGTTTTGCTCAGATTGGTGTGGGAAG
AGCCTGCCTGTGGGGAGCGGCCACTCCATACTGCTGAGGCCTCAGGACTGCTGCTCAGCT TGCCCGTTACCTGAAGAGGCGGCGGAGCCGGGCCCCTGACCGGTCACCATGTGGGCCTTC TCGGAATTGCCCATGCCGCTGCTGATCAATTTGATCGTCTCGCTGCTGGGATTTGTGGCC ACAGTCACCCTCATCCCGGCCTTCCGGGGCCACTTCATTGCTGCGCGCCTCTGTGGTCAG GACCTCAACAAAACCAGCCGACAGCAGATCCCAGAATCCCAGGGAGTGATCAGCGGTGCT GTTTTCCTTATCATCCTCTTCTGCTTCATCCCTTTCCCCTTCCTGAACTGCTTTGTGAAG GAGCAGTGTAAGGCATTCCCCCACCATGAATTTGTGGCCCTGATAGGTGCCCTCCTTGCC ATCTGCTGCATGATCTTCCTGGGCTTTGCGGATGATGTACTGAATCTGCGCTGGCGCCAT AAGCTGCTGCTACCTACAGCTGCCTCACTACCTCTCCTCATGGTCTATTTCACCAACTTT GGCAACACGACCATTGTGGTGCCCAAGCCCTTCCGCCCGATACTTGGCCTGCATCTGGAC TTGGGAATCCTGTACTATGTCTACATGGGGCTGCTGGCAGTGTTCTGTACCAATGCCATC AATATCCTAGCAGGAATTAACGGCCTAGAGGCTGGCCAGTCACTAGTCATTTCTGCTTCC ATCATTGTCTTCAACCTGGTAGAGTTGGAAGGTGATTGTCGGGATGATCATGTCTTTTCC CTCTACTTCATGATACCCTTTTTTTTCACCACTTTGGGATTGCTCTACCACAACTGGTAC CCATCACGGGTGTTTGTGGGAGATACCTTCTGTTACTTTGCTGGCATGACCTTTGCCGTG GTGGGCATCTTGGGACACTTCAGCAAGACCATGCTACTATTCTTCATGCCCCAGGTGTTC AACTTCCTCTACTCACTGCCTCAGCTCCTGCATATCATCCCCTGCCCTCGCCACCGCATA CCCAGACTCAATATCAAGACAGGCAAACTGGAGATGAGCTATTCCAAGTTCAAGACCAAG AGCCTCTCTTTCTTGGGCACCTTTATTTTAAAGGTGGCAGAGAGCCTCCAGCTGGTGACA GTACACCAGAGTGAGACTGAAGATGGTGAATTCACTGAATGTAACAACATGACCCTCATC AACTTGCTACTTAAAGTCCTTGGGCCCATACATGAGAGAAACCTCACATTGCTCCTGCTG CTGCTGCAGATCCTGGGCAGTGCCATCACCTTCTCCATTCGATATCAGCTCGTTCGACTC TTCTATGATGTCTGAGTCCCTTGATCATTGTCCTTTACCTCACAGTCTCTAGGATTCCTG ACTCAGGCTGACCTCTCTCTCTGGTCCCAGACTGCCTCCTTGCCCAGGCCTCTCTCACTC TTCATACTCCTCCAGATTTTGTTCTCAGCATTTTCCTTTCTCTGTGATCATTGGCATCCT GGGCGTTTCTTGCCCTCTGCTGACTACTGATTGGATTTTACCTATGGCTTTCTGCAACTT GCTACTCTCTCCCTCTCCATCCCATCTTTGCAGCCTCATAGGGTGGGATACAGCAGCTTT TTTTGCAGTTATCCACACTCACATTTCAGAGTCCTGACTCTCAAGGAACCACTGGTTTTT GGGATAGAACTTGGGCCAGGGCTAGGAACACAGGCTCCACGGTGACATGTCATTTGATTG TAAATTAAGTGTTCTGATTAGTAAGAACTAAGCAGGGGGCCACATGCTCTCAATGGAGAC AATAAAGTGTTGTCTTTTTCTTATAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_001382 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001382.2, NP_001373.2 |
RefSeq Size | 2159 bp |
RefSeq ORF | 1227 bp |
Locus ID | 1798 |
UniProt ID | Q9H3H5 |
Domains | Glycos_transf_4 |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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N-Glycosylation Induces the CTHRC1 Protein and Drives Oral Cancer Cell Migration
,Gangli Liu, Pritam K. Sengupta, Basem Jamal, Hsiao-Ying Yang, Meghan P. Bouchie, Volkhard Lindner, Xaralabos Varelas, and Maria A. Kukuruzinska,
J. Biol. Chem., Jul 2013; 288: 20217 - 20227.
,PubMed ID 23703614
[DPAGT1]
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Other Versions
SKU | Description | Size | Price |
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RC201787 | DPAGT1 (Myc-DDK-tagged)-Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1) |
CNY 3,656.00 |
|
RC201787L1 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC201787L2 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), mGFP tagged |
CNY 5,890.00 |
|
RC201787L3 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201787L4 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), mGFP tagged |
CNY 5,890.00 |
|
RG201787 | DPAGT1 (tGFP-tagged) - Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1) |
CNY 5,256.00 |