DDX50 (NM_024045) Human Untagged Clone
CAT#: SC320213
DDX50 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 (DDX50)
CNY 5,408.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GU2; GUB; mcdrh; RH-II/GuB |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_024045.1
GCATGGCCAAGGCCGGGCGGCCGCCTTGCCCCCGCTTCCTTTCACGCTGTCGCTGCCCGT
AGGTGGTTGTGGCCACTGTGCCCGGAGGGAGGCGGCGGTGGCCAGTAATGCCTGGGAAAC TCCTCTGGGGGGACATTATGGAGCTGGAAGCACCCTTGGAGGAGTCCGAGAGCCAGAAGA AGGAGAGGCAAAAGAGTGACAGAAGGAAGTCAAGGCACCATTATGACTCGGATGAGAAAT CAGAAACAAGAGAAAATGGTGTTACAGATGACCTGGATGCTCCCAAGGCCAAAAAATCTA AAATGAAAGAGAAGCTAAATGGAGACACTGAAGAAGGATTTAATAGACTTTCAGATGAAT TCTCCAAATCTCATAAGTCAAGAAGAAAAGATCTACCAAATGGAGATATAGATGAATATG AAAAAAAATCAAAGCGAGTATCATCTTTAGATACTTCTACTCATAAATCAAGTGATAATA AACTAGAGGAGACCTTAACACGTGAACAGAAAGAAGGAGCCTTCTCCAATTTTCCTATTT CTGAAGAGACTATAAAGCTTCTGAAAGGTCGAGGGGTAACATATCTCTTTCCTATTCAAG TTAAGACCTTTGGTCCTGTATATGAAGGAAAAGATTTAATAGCTCAAGCACGGACAGGAA CAGGAAAGACATTCTCTTTTGCCATCCCCTTAATTGAAAGACTCCAAAGAAATCAAGAAA CAATTAAAAAAAGCCGCTCACCAAAGGTACTTGTTTTGGCTCCAACAAGGGAACTGGCAA ACCAAGTAGCCAAAGACTTCAAAGATATAACTAGGAAACTCAGCGTGGCGTGTTTTTATG GTGGAACATCATATCAAAGCCAAATTAATCATATTCGAAATGGTATTGACATCTTGGTTG GAACACCTGGTCGTATCAAAGACCATCTGCAGAGTGGCCGATTGGATCTTTCTAAACTGC GACATGTTGTGCTTGATGAAGTGGATCAGATGTTAGATTTAGGTTTCGCTGAACAAGTTG AAGATATTATTCATGAATCCTACAAAACTGATTCTGAAGACAATCCTCAGACTTTACTTT TTTCTGCAACTTGCCCACAGTGGGTATACAAAGTTGCAAAAAAATACATGAAATCCAGAT ATGAACAGGTTGACCTTGTTGGAAAAATGACTCAAAAGGCTGCAACTACTGTGGAACATT TGGCCATCCAGTGTCATTGGTCTCAGAGGCCAGCAGTTATTGGAGATGTCCTTCAAGTCT ACAGTGGGTCTGAAGGGAGGGCTATTATTTTCTGTGAGACCAAGAAGAATGTAACTGAAA TGGCCATGAATCCACACATAAAACAGAATGCCCAGTGTTTACATGGGGACATTGCACAGT CACAAAGAGAAATTACACTAAAAGGCTTCAGAGAAGGTAGTTTTAAAGTTTTGGTGGCAA CCAATGTGGCTGCCCGTGGTTTGGACATTCCTGAAGTTGACCTGGTGATTCAAAGTTCTC CTCCTCAGGATGTTGAGTCCTATATCCATCGCTCTGGACGCACAGGTAGAGCTGGACGGA CAGGGATTTGTATATGTTTTTATCAACCAAGAGAAAGAGGTCAACTAAGATATGTGGAAC AAAAAGCAGGAATTACTTTTAAACGTGTAGGTGTTCCTTCTACAATGGATTTAGTTAAAT CTAAAAGCATGGATGCCATCAGGTCTCTGGCTTCCGTTTCTTATGCTGCTGTTGATTTTT TCCGACCATCAGCTCAGAGACTGATAGAAGAGAAAGGTGCAGTGGATGCATTGGCTGCAG CTTTAGCCCACATTTCTGGTGCATCAAGCTTTGAACCACGATCTTTGATCACCTCTGATA AGGGGTTTGTGACCATGACTCTGGAAAGCCTAGAGGAAATACAGGATGTCAGCTGTGCTT GGAAAGAACTTAACAGAAAGCTGAGTAGTAATGCAGTGTCTCAGATTACCAGAATGTGCC TCCTGAAAGGAAATATGGGTGTTTGCTTTGATGTTCCTACAACTGAGTCAGAAAGGTTAC AGGCAGAGTGGCATGATTCCGACTGGATACTCTCAGTGCCAGCCAAATTACCTGAAATTG AAGAATATTATGATGGAAACACATCTTCTAATTCCAGACAGAGGAGTGGCTGGTCAAGTG GTCGATCAGGCCGGTCAGGCCGGTCAGGTGGTCGATCTGGCGGCCGGTCAGGTAGACAGA GTCGACAAGGAAGTCGCTCAGGAAGTCGACAAGATGGTAGAAGACGAAGTGGGAATAGAA ATCGATCAAGAAGTGGGGGCCACAAACGGAGTTTTGACTGAGTATTTGATAGTTAATCTA CCAGTGTGAGCTTGCCTATTTCTGCCTAATCATGTACATTATCCACCAAAAATTAGGTCA TCATAGTTGAGGTATGTGTCTGCTATTTGCAAAGAAGTTGGTCGTATTTTTTTAAAAAGT ATTTCACAAGAATGGAACAAATCTACTTATCCAGTTATACCTTTGAATAAAAAAACCTCC TTTTATTGTCTCTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_024045 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_024045.1, NP_076950.1 |
RefSeq Size | 2575 bp |
RefSeq ORF | 2214 bp |
Locus ID | 79009 |
UniProt ID | Q9BQ39 |
Domains | DEAD, helicase_C |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200990 | DDX50 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 (DDX50) |
CNY 5,400.00 |
|
RC200990L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 (DDX50), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200990L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 (DDX50), mGFP tagged |
CNY 5,890.00 |
|
RG200990 | DDX50 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 (DDX50) |
CNY 7,000.00 |