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PRAK (MAPKAPK5) (NM_139078) Human Untagged Clone
CAT＃: SC319941
MAPKAPK5 (untagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 2
CNY 3,656.00
Product images
CNY 3,990.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAPKAP-K5; MK-5; MK5; PRAK |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_139078.1
GTGGCGCTGAGGCGGCGGCGGGAGCAGCGGCGCCGAGCTCTGCTTCGGCTTCGGCTTCGG
CTTCGCGGCGGTGCAGGCGGCGGAGGCGGAGGCGCAGGCTCTTCTTTAGGACCTGGCGAG CCCAGGTCTAAGCGGCGGCCCCGCGAGGCCCTTGCACGGCGCCCCGGGTCGAGGCCCTCC CCGCCTCGCCCTACCCCAGGAGCCTGCCTCCCCAGCTGGGGATGAGGCTAGGAGGCGGCC GCGTGGGGCCCAGCACAAAGACCTGTCCCCAGGGGCCGCCGCCTCCGCCGCTGCTGCTGC CGCCAGCCTAGAGCCGCCCGCCGAAGCAGAGCCGGCGCCGGGGTCCTCATCCCCACCGGT CCCGAGGGGCGGCTGCTGCCCGTCGCCACGAGGCCCAGGGGCCCGAGTGCCGAGCCCTTT GCTCCCTCGGCCGCGCGGGGACAGGGCTGCTGAGCAGCCTCCGCCTCTCCCGGCTGTGGG GGCCCCACTGAGTATGTCGGAGGAGAGCGACATGGACAAAGCCATCAAGGAAACTTCCAT TTTAGAAGAATACAGTATCAATTGGACTCAGAAGCTGGGAGCTGGAATTAGTGGTCCAGT TAGAGTCTGTGTAAAGAAATCTACTCAAGAACGGTTTGCGCTGAAAATTCTTCTTGATCG TCCAAAAGCTAGAAATGAGGTACGTCTGCACATGATGTGTGCCACACACCCAAACATAGT TCAGATTATTGAAGTGTTTGCTAACAGTGTCCAGTTTCCCCATGAGTCCAGCCCTAGGGC CCGACTCTTAATTGTAATGGAGATGATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCA GCACCGGCACTTTACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCT GCGGCACTGTCACTTGTTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTT TAAGGATAACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGA CCAAGGTGACTTGATGACACCCCAGTTCACCCCTTATTATGTAGCACCCCAGGTACTGGA GGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTA CACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTG CGGATACCCTCCTTTTTACTCCAAACACCACAGCCGGACTATCCCAAAGGATATGCGAAG AAAGATCATGACAGGCAGTTTTGAGTTCCCAGAGGAAGAGTGGAGTCAGATCTCAGAGAT GGCCAAAGATGTTGTGAGGAAGCTCCTGAAGGTCAAACCGGAGGAGAGACTCACCATCGA GGGAGTGCTGGACCACCCCTGGCTCAATTCCACCGAGGCCCTGGATAATGTGCTGCCTTC TGCTCAGCTGATGATGGACAAGGCAGTGGTTGCAGGAATCCAGCAGGCTCACGCGGAACA GTTGGCCAACATGAGAATCCAGGATCTGAAAGTCAGCCTCAAACCCCTGCACTCAGTGAA CAACCCCATTCTGCGGAAGAGGAAGTTACTTGGCACCAAGCCAAAGGACAGTGTCTATAT CCACGACCATGAGAATGGAGCCGAGGATTCCAATGTTGCCTTGGAAAAACTCCGAGATGT GATTGCTCAGTGTATTCTCCCCCAGGCTGGAGAGAATGAAGATGAGAAACTGAATGAAGT AATGCAGGAGGCTTGGAAGTATAACCGGGAATGCAAACTCCTAAGAGATACTCTGCAGAG CTTCAGCTGGAATGGTCGTGGATTCACAGATAAAGTAGATCGACTAAAACTGGCAGAAAT TGTGAAGCAGGTGATAGAAGAGCAAACCACGTCCCACGAATCCCAATAATGACAGCTTCA GACTTTGTTTTTTTAACAATTTGAAAAATTATTCTTTAATGTATAAAGTAATTTTATGTA AATTAATAAATCATAATTTCATTTCCAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_139078 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139078.1, NP_620777.1 |
| RefSeq Size | 2066 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 8550 |
| UniProt ID | Q8IW41 |
| Domains | pkinase |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (2) contains an extra 6 nt segment in the coding region when compared to variant 1. It encodes an isoform (2) longer by 2 aa, as compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208304 | MAPKAPK5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 2 |
CNY 3,990.00 |
|
| RC208304L3 | Lenti-ORF clone of MAPKAPK5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 2 |
CNY 5,890.00 |
|
| RC208304L4 | Lenti-ORF clone of MAPKAPK5 (mGFP-tagged)-Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 2 |
CNY 5,890.00 |
|
| RG208304 | MAPKAPK5 (tGFP-tagged) - Human mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 2 |
CNY 4,370.00 |
