trfp (MED20) (NM_004275) Human Untagged Clone

CAT#: SC319829

MED20 (untagged)-Human mediator complex subunit 20 (MED20)



  "NM_004275" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-MED20 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PRO0213; SRB2; TRFP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_004275.3 GGGGGTATTTCCGGAGTCCCTTCTGAGACAGAAGGTTGTGTTGGGGAACTGAAGGAGTTT
CTGTGGGCGGACAGGGAACCCTGCGTTTCTACTGTGTGATTCTGCCACCTTCCTGGCCCG
ACGCCATGGGAGTGACTTGTGTGTCCCAGATGCCTGTGGCCGAGGGCAAGAGTGTTCAGC
AAACCGTAGAGCTCCTTACCCGGAAATTGGAGATGCTTGGGGCAGAGAAGCAAGGAACAT
TTTGTGTGGACTGTGAGACTTACCATACGGCCGCCTCTACCCTTGGCAGCCAAGGTCAGA
CCGGGAAGCTGATGTATGTGATGCACAACTCAGAGTACCCATTGAGCTGTTTCGCCCTCT
TTGAGAATGGCCCTTGCCTTATTGCTGACACCAACTTTGATGTGCTTATGGTGAAGCTCA
AGGGCTTTTTCCAGAGTGCTAAGGCCAGCAAGATTGAGACCCGGGGCACCAGGTACCAGT
ACTGTGACTTCCTGGTGAAGGTGGGCACGGTCACAATGGGGCCCAGTGCCCGGGGCATCT
CTGTGGAGGTGGAGTATGGCCCCTGTGTGGTAGCTAGTGACTGCTGGAGTCTGCTGCTCG
AGTTCCTACAGAGTTTTCTAGGCAGCCACACACCAGGGGCTCCCGCAGTGTTTGGGAACA
GACATGATGCGGTCTACGGCCCAGCAGATACCATGGTCCAGTACATGGAACTCTTCAACA
AGATCCGCAAGCAGCAGCAGGTGCCGGTGGCTGGGATTCGTTAGTGATGAGCAGCTGCCA
GCTGAGCTCTGTCACCAGGGGTACTCCACAGGAGGAGCAGGTGCTGACTTTCAGGTCCCT
GGACCCCAGAAACCCAGGGTAGACATGGACTCTACTCTCCTTCTCTGGTTCTCAGCTGTG
GCTTTTGTTCTGGGGCTGAGTCCCCTCCCCAACCCCCTGACTCTCACACATAGCCCCCCA
TCAGCTGTTTTACTCCGTGCCTTACTGGATTTGGCCTGTCCTCAAGAATGGTAATTATGA
AGAGTGGAGAAGTTTGGACCTTGCCTCCTTTAGGGGAAAGGTAGGACAGGAATGTCCCTG
CCTGGTACTGGTGGGGGAAATAGTCCGTATCCCCAACTATTAAGGATTTGTCCCAGGCCA
ATGGTGAACATGCTGCATTTTATGTTTGGATTGTGCTGTAATAAGAGCCTCTTCCTTCCC
TCAGAGGATGTGGCTGGGCTTCTATCCTAGAGATGGAGTAGAAGGCACAACTGGTTTGAT
AGTTACTATTTTCTGACCTGTTTGCTGAAGTGATTTGCGAATTGACTTTCCTAGGGTGTT
GCCTGAGTCCTTTGAAATCTCCTTCTGACATCTTTCCCTTCTGTTGTGAAATATGTTAAG
CCACAGGCCAAACTGCTATAAGATCGAAGTTTGTTTTTTTCTCACCTAATAATAGAGAGG
TAATCAGATGGTCTAGGGCAGATTTAACTCTACAAGGTTATCAGGAACTTTGTTTCCTTC
TGTCTTATTCAGCCATTCCCAGTATGTTTTCTTACCCATAAGATCAAAGCAGACTCATCA
GCTCCATATCTGCATTCCACTGGGGAAAGAGAGAGAGAAAAGAGCAGATTTTTAAAAAAA
GATATGACTGAGCATTGCTCAGATCACTGTGGCCACACTTTGCTGAAAGAGATGCTGGGA
CATAGAACCTCTTATCTGGGCAGTCATGTGCTGAGTTAAAACTCAGGGATTCTGTGACTA
CAAGATGAAAGACAATTTGGTTGCTGAGATGCAGTTAATGGCTTCTGTTACATGGAGCTC
AATGGACGTGCCCAGGAATGCTTTTGGCTGTTATTTTGCAGTTAATACCTCCTGTAACTA
AAGCATTTGTTTATGAGTTGACTTGAGAGAAGGGCTGATCTCAGAGCCGCTTTGAGCTAA
GTTGGATTAGTCACACTAGGAAGTTAATTCCACACCTTTCGTCTAAGTCTCAGTATTGAG
GCCTCTCCAGTTCTCATGCACCCTGATCTTAGGGTTAGAATACTTGACCCTGATACCTGC
ACCATGCTTCATGGTTCCTGAGCTCTTTCTCCTGTTTCATTTGAGCCTCCAAACTACATA
TTTGGTCATATTGCCTGCCTACCCCATGCCTGCTGCAGAAATATTCATCCAGGTTAACCT
TGATATACACAGAGATGGTCTTGGAGAATTGTGAATGTATGTACTGTATTGTCATCAAGG
ATACTGTCCCTTATTTGAAGGCATCTAAAGAGAAACTGTTTTCAGATCCAAGTGCTCAGA
TCTAAAGCCTCTGCAACAAGTCAGGTGGTGGTCATGTTTCCCTTCTAGTTTTGGCTGACA
GGAAGCTCAGTTCAGTACCATAACTACAGAACCTGTCATCTGTATTTTTTGTTCTCACCC
CGTTTTTGTTATTTTGTTTCTGGTTTTTTATATTGAGGTATGTTTTAGATATAGTTTACA
AAAATAAAACGCACAGATCTTACAAAAAAAAAAAAATAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_004275
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004275.3, NP_004266.2
RefSeq Size 2478 bp
RefSeq ORF 639 bp
Locus ID 9477
UniProt ID Q9H944
Gene Summary This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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