trfp (MED20) (NM_004275) Human Untagged Clone
CAT#: SC319829
MED20 (untagged)-Human mediator complex subunit 20 (MED20)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | PRO0213; SRB2; TRFP |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_004275.3
GGGGGTATTTCCGGAGTCCCTTCTGAGACAGAAGGTTGTGTTGGGGAACTGAAGGAGTTT
CTGTGGGCGGACAGGGAACCCTGCGTTTCTACTGTGTGATTCTGCCACCTTCCTGGCCCG ACGCCATGGGAGTGACTTGTGTGTCCCAGATGCCTGTGGCCGAGGGCAAGAGTGTTCAGC AAACCGTAGAGCTCCTTACCCGGAAATTGGAGATGCTTGGGGCAGAGAAGCAAGGAACAT TTTGTGTGGACTGTGAGACTTACCATACGGCCGCCTCTACCCTTGGCAGCCAAGGTCAGA CCGGGAAGCTGATGTATGTGATGCACAACTCAGAGTACCCATTGAGCTGTTTCGCCCTCT TTGAGAATGGCCCTTGCCTTATTGCTGACACCAACTTTGATGTGCTTATGGTGAAGCTCA AGGGCTTTTTCCAGAGTGCTAAGGCCAGCAAGATTGAGACCCGGGGCACCAGGTACCAGT ACTGTGACTTCCTGGTGAAGGTGGGCACGGTCACAATGGGGCCCAGTGCCCGGGGCATCT CTGTGGAGGTGGAGTATGGCCCCTGTGTGGTAGCTAGTGACTGCTGGAGTCTGCTGCTCG AGTTCCTACAGAGTTTTCTAGGCAGCCACACACCAGGGGCTCCCGCAGTGTTTGGGAACA GACATGATGCGGTCTACGGCCCAGCAGATACCATGGTCCAGTACATGGAACTCTTCAACA AGATCCGCAAGCAGCAGCAGGTGCCGGTGGCTGGGATTCGTTAGTGATGAGCAGCTGCCA GCTGAGCTCTGTCACCAGGGGTACTCCACAGGAGGAGCAGGTGCTGACTTTCAGGTCCCT GGACCCCAGAAACCCAGGGTAGACATGGACTCTACTCTCCTTCTCTGGTTCTCAGCTGTG GCTTTTGTTCTGGGGCTGAGTCCCCTCCCCAACCCCCTGACTCTCACACATAGCCCCCCA TCAGCTGTTTTACTCCGTGCCTTACTGGATTTGGCCTGTCCTCAAGAATGGTAATTATGA AGAGTGGAGAAGTTTGGACCTTGCCTCCTTTAGGGGAAAGGTAGGACAGGAATGTCCCTG CCTGGTACTGGTGGGGGAAATAGTCCGTATCCCCAACTATTAAGGATTTGTCCCAGGCCA ATGGTGAACATGCTGCATTTTATGTTTGGATTGTGCTGTAATAAGAGCCTCTTCCTTCCC TCAGAGGATGTGGCTGGGCTTCTATCCTAGAGATGGAGTAGAAGGCACAACTGGTTTGAT AGTTACTATTTTCTGACCTGTTTGCTGAAGTGATTTGCGAATTGACTTTCCTAGGGTGTT GCCTGAGTCCTTTGAAATCTCCTTCTGACATCTTTCCCTTCTGTTGTGAAATATGTTAAG CCACAGGCCAAACTGCTATAAGATCGAAGTTTGTTTTTTTCTCACCTAATAATAGAGAGG TAATCAGATGGTCTAGGGCAGATTTAACTCTACAAGGTTATCAGGAACTTTGTTTCCTTC TGTCTTATTCAGCCATTCCCAGTATGTTTTCTTACCCATAAGATCAAAGCAGACTCATCA GCTCCATATCTGCATTCCACTGGGGAAAGAGAGAGAGAAAAGAGCAGATTTTTAAAAAAA GATATGACTGAGCATTGCTCAGATCACTGTGGCCACACTTTGCTGAAAGAGATGCTGGGA CATAGAACCTCTTATCTGGGCAGTCATGTGCTGAGTTAAAACTCAGGGATTCTGTGACTA CAAGATGAAAGACAATTTGGTTGCTGAGATGCAGTTAATGGCTTCTGTTACATGGAGCTC AATGGACGTGCCCAGGAATGCTTTTGGCTGTTATTTTGCAGTTAATACCTCCTGTAACTA AAGCATTTGTTTATGAGTTGACTTGAGAGAAGGGCTGATCTCAGAGCCGCTTTGAGCTAA GTTGGATTAGTCACACTAGGAAGTTAATTCCACACCTTTCGTCTAAGTCTCAGTATTGAG GCCTCTCCAGTTCTCATGCACCCTGATCTTAGGGTTAGAATACTTGACCCTGATACCTGC ACCATGCTTCATGGTTCCTGAGCTCTTTCTCCTGTTTCATTTGAGCCTCCAAACTACATA TTTGGTCATATTGCCTGCCTACCCCATGCCTGCTGCAGAAATATTCATCCAGGTTAACCT TGATATACACAGAGATGGTCTTGGAGAATTGTGAATGTATGTACTGTATTGTCATCAAGG ATACTGTCCCTTATTTGAAGGCATCTAAAGAGAAACTGTTTTCAGATCCAAGTGCTCAGA TCTAAAGCCTCTGCAACAAGTCAGGTGGTGGTCATGTTTCCCTTCTAGTTTTGGCTGACA GGAAGCTCAGTTCAGTACCATAACTACAGAACCTGTCATCTGTATTTTTTGTTCTCACCC CGTTTTTGTTATTTTGTTTCTGGTTTTTTATATTGAGGTATGTTTTAGATATAGTTTACA AAAATAAAACGCACAGATCTTACAAAAAAAAAAAAATAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_004275 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004275.3, NP_004266.2 |
RefSeq Size | 2478 bp |
RefSeq ORF | 639 bp |
Locus ID | 9477 |
UniProt ID | Q9H944 |
Gene Summary | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203152 | MED20 (Myc-DDK-tagged)-Human mediator complex subunit 20 (MED20) |
CNY 2,400.00 |
|
RC203152L1 | Lenti ORF clone of Human mediator complex subunit 20 (MED20), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC203152L2 | Lenti ORF clone of Human mediator complex subunit 20 (MED20), mGFP tagged |
CNY 5,890.00 |
|
RC203152L3 | Lenti ORF clone of Human mediator complex subunit 20 (MED20), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203152L4 | Lenti ORF clone of Human mediator complex subunit 20 (MED20), mGFP tagged |
CNY 5,890.00 |
|
RG203152 | MED20 (tGFP-tagged) - Human mediator complex subunit 20 (MED20) |
CNY 4,370.00 |