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MAN2B1 (NM_000528) Human Untagged Clone
CAT＃: SC319430
MAN2B1 (untagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1
CNY 7,048.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LAMAN; MANB |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000528.2
CCCAGGAGGAAGCTGCTGAGCCATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCG
CGGCTGCCTGGACTCAGCAGGCCCCTGGACCATGTCCCGCGCCCTGCGGCCACCGCTCCC GCCTCTCTGCTTTTTCCTTTTGTTGCTGGCGGCTGCCGGTGCTCGGGCCGGGGGATACGA GACATGCCCCACAGTGCAGCCGAACATGCTGAACGTGCACCTGCTGCCTCACACACATGA TGACGTGGGCTGGCTCAAAACCGTGGACCAGTACTTTTATGGAATCAAGAATGACATCCA GCACGCCGGTGTGCAGTACATCCTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCAC CCGTCGCTTCATTTACGTGGAGATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAA TGCCACACAGGAAGTCGTGCGAGACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGG TGGCTGGGTGATGAACGATGAGGCAGCCACCCACTACGGTGCCATCGTGGACCAGATGAC ACTTGGGCTGCGCTTTCTGGAGGACACATTTGGCAATGATGGGCGACCCCGTGTGGCCTG GCACATTGACCCCTTCGGCCACTCTCGGGAGCAGGCCTCGCTGTTTGCGCAGATGGGCTT CGACGGCTTCTTCTTTGGGCGCCTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCT GGAGATGGAGCAGGTGTGGCGGGCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTT CACTGGTGTGCTTCCCAATGGTTACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTG TGTCGATCAGCCGCTGGTGGAGGACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGT CGATTACTTCCTAAATGTGGCCACTGCCCAGGGCCGGTATTACCGCACCAACCACACTGT GATGACCATGGGCTCGGACTTCCAATATGAGAATGCCAACATGTGGTTCAAGAACCTTGA CAAGCTCATCCGGCTGGTAAATGCGCAGCAGGCAAAAGGAAGCAGTGTCCATGTTCTCTA CTCCACCCCCGCTTGTTACCTCTGGGAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAA ACATGACGACTTCTTCCCTTACGCGGATGGCCCCCACCAGTTCTGGACCGGTTACTTTTC CAGTCGGCCGGCCCTCAAACGCTACGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAA CCAGCTGGAGGCGCTGGTGGGCCTGGCGGCCAACGTGGGACCCTATGGCTCCGGAGACAG TGCACCCCTCAATGAGGCGATGGCTGTGCTCCAGCATCACGACGCCGTCAGCGGCACCTC CCGCCAGCACGTGGCCAACGACTACGCGCGCCAGCTTGCGGCAGGCTGGGGGCCTTGCGA GGTTCTTCTGAGCAACGCGCTGGCGCGGCTCAGAGGCTTCAAAGATCACTTCACCTTTTG CCAACAGCTAAACATCAGCATCTGCCCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCAT CGTTTATAATCCCCTGGGGCGGAAGGTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGG CGTTTTCGTTGTGAAGGACCCCAATGGCAGGACAGTGCCCAGCGATGTGGTAATATTTCC CAGCTCAGACAGCCAGGCGCACCCTCCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCT GGGCTTCAGCACCTATTCAGTAGCCCAGGTGCCTCGCTGGAAGCCCCAGGCCCGCGCACC ACAGCCCATCCCCAGAAGATCCTGGTCCCCTGCTTTAACCATCGAAAATGAGCACATCCG GGCAACGTTTGATCCTGACACAGGGCTGTTGATGGAGATTATGAACATGAATCAGCAACT CCTGCTGCCTGTTCGCCAGACCTTCTTCTGGTACAACGCCAGTATAGGTGACAACGAAAG TGACCAGGCCTCAGGTGCCTACATCTTCAGACCCAACCAACAGAAACCGCTGCCTGTGAG CCGCTGGGCTCAGATCCACCTGGTGAAGACACCCTTGGTGCAGGAGGTGCACCAGAACTT CTCAGCTTGGTGTTCCCAGGTGGTTCGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGA GTGGTCGGTGGGGCCGATACCTGTGGGCGACACCTGGGGGAAGGAGGTCATCAGCCGTTT TGACACACCGCTGGAGACAAAGGGACGCTTCTACACAGACAGCAATGGCCGGGAGATCCT GGAGAGGAGGCGGGATTATCGACCCACCTGGAAACTGAACCAGACGGAGCCCGTGGCAGG AAACTACTATCCAGTCAACACCCGGATTTACATCACGGATGGAAACATGCAGCTGACTGT GCTGACTGACCGCTCCCAGGGGGGCAGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGT GCACCGAAGGCTGCTGAAGGACGATGGACGCGGAGTATCGGAGCCACTAATGGAGAACGG GTCGGGGGCGTGGGTGCGAGGGCGCCACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGC CGCCGGACACCGGCTCCTGGCGGAGCAGGAGGTCCTGGCCCCTCAGGTGGTGCTGGCCCC GGGTGGCGGCGCCGCCTACAATCTCGGGGCTCCTCCGCGCACGCAGTTCTCAGGGCTGCG CAGGGACCTGCCGCCCTCGGTGCACCTGCTCACGCTGGCCAGCTGGGGCCCCGAAATGGT GCTGCTGCGCTTGGAGCACCAGTTTGCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGC CCCCGTTACCTTGAACTTGAGGGACCTGTTCTCCACCTTCACCATCACCCGCCTGCAGGA GACCACGCTGGTGGCCAACCAGCTCCGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAA CACAGGCCCCACACCCCACCAAACTCCGTACCAGCTGGACCCGGCCAACATCACGCTGGA ACCCATGGAAATCCGCACTTTCCTGGCCTCAGTTCAATGGAAGGAGGTGGATGGTTAGGT CTGCTGGGATGGGCCCTCCAAGCCCAAGCCTCCTGCTCCGGGGGCAGACCAGACTCTGAC TCTCCTCTTGGGGCTGCTGCCATTAAAACGCTACTACTAAGACTCAAAAAAAAAAAAAAA AAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000528 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000528.2, NP_000519.2 |
| RefSeq Size | 3468 bp |
| RefSeq ORF | 3036 bp |
| Locus ID | 4125 |
| UniProt ID | O00754 |
| Domains | Glyco_hydro_38 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Other glycan degradation |
| Gene Summary | This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200638 | MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1 |
CNY 7,040.00 |
|
| RC200638L1 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, Myc-DDK-tagged |
CNY 9,440.00 |
|
| RC200638L2 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RC200638L3 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC200638L4 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RG200638 | MAN2B1 (tGFP-tagged) - Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1 |
CNY 9,500.00 |
