LIMCH1 (NM_001112718) Human Untagged Clone
CAT#: SC319018
LIMCH1 (untagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 3
CNY 20,330.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | LIMCH1A; LMO7B |
Vector | pCMV6 series |
Sequence Data |
>NCBI ORF sequence for NM_001112718, the custom clone sequence may differ by one or more nucleotides
ATGGCTTGTCCCGCTCTCGGTCTGGAAGCTCTTCAGCCCCTGCAGCCCGAGCCGCCCCCC GAGCCCGCCTTCTCCGAGGCGCAGAAGTGGATTGAGCAAGTAACTGGCAGAAGTTTTGGT GATAAAGATTTTCGGACAGGTTTAGAAAATGGAATCCTCCTCTGCGAGTTGCTGAATGCT ATAAAGCCAGGACTTGTTAAAAAGATCAATAGATTGCCTACCCCCATTGCAGGACTGGAC AATATTATCTTATTCTTGAGAGGTTGTAAAGAGCTCGGCCTTAAAGAATCTCAACTTTTT GACCCGAGTGACCTCCAGGATACATCCAACAGAGTAACAGTCAAGAGCCTTGATTATAGT AGGAAGCTGAAAAATGTATTAGTTACCATTTACTGGCTGGGAAAAGCAGCAAACAGCTGC ACATCCTACAGCGGAACGACACTAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATG CGAAAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATC GACTGCTGGGATTCCGAGCGCAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGAT TCCTTCGACAGCCTGGATTCCTTTGGCTCTCGCTCTCGGCAGACGCCTTCACCAGATGTA GTCCTCAGGGGAAGCAGCGATGGGAGAGGAAGCGACTCTGAATCCGACTTGCCTCATCGG AAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGGCGGACTTCCCATGGTGAGCCG AAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCAGACGGCCTACGTC CCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGTACC GCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGATACGGTCCGAGAACTCCTGTGTCT GATGACGCAGAGAGCACCAGCATGTTTGACATGCGGTGTGAGGAGGAGGCCGCGGTGCAG CCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAG GACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAG GACTTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGCTGGAGAAGAT GGGACAAGTGAACGAAGGAAAAGCATCAAAACCTACAGAGAAATTGTTCAAGAAAAAGAG CGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAACGCTCGGTCCCAGGAGGAGGCAGAG GGGATCCTTCAACAGTACATTGAGAGGTTCACCATCAGTGAGGCTGTTCTCGAACGCTTG GAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCAAATTTATCCTCCTTCCTG AATGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTGCCTCCACCCAAATTCACT GCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCAGCAGGC AGTGGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCTATGCTGACACCCAAGCCT TACTCCCAGCCCAAAAATTCTCAAGATGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTC AGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAGAAGGAAAGAGAGTGTCCCACGGTG GCACCTGCCCACTCCTTAACCAAATCCCAGATGTTTGAAGGTGTGGCCAGAGTGCACGGG TCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATCAACATAAAGAAGCCAAAC TCTGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAACCGAATAGTCAAGAGGAC AAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCATCAGAACCACAG CATTTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCCTCC AGCCCCCAGCTGAAGAATGATGTGTCGGAAGAAAAAGACCAGAAGAAACCAGAAAATGAA ATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTGGTAAAGCCAAAATCTCCAGAA CCCGAAGCAACGCTGACATTTCCATTTCTGGACAAAATGCCTGAAGCCAACCAACTACAT TTGCCAAATCTCAATTCTCAAGTGGATTCTCCAAGCAGTGAGAAGTCACCTGTTATGACA CCTTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGGCGACAGGAAAAATGGCAA CAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGAAGGAGCAGGACAAGCTGAAAGAA GAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATACTATGAGGAGGAG CGTAAGATAATTGAAGACACTGTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGACCAG CTGTCTACCTCTTCCTCCATGACTGAAGGCAGTGGGACAATGAATAAGATAGACCTGGGA AACTGTCAAGATGAAAAACAAGACAGAAGATGGAAGAAATCATTCCAGGGAGATGACAGT GACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGAGGAGAAGGGCAGCCTAACTGAA GGGGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTCCATGAAGACCATCAGCTG GATACCGAGGCTGGGGCCCCACACTGTGGAACAAACCCACAGCTTGCTCAGGATCCATCC CAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAAGATGTGAAGCCAAAAACCCTC CCGCTGGATAAAAGCATTAACCATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGTCTATA AGTGGAAAGAAGCTGTGCTCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATGATC ATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAGGTGTGGAATTTGTAAAGGC CAGCTTGGAGATGCAGTGAGTGGGACGGATGTTAGGATTCGAAATGGTCTCCTGAACTGT AATGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACAACATTG |
Restriction Sites | Please inquire |
ACCN | NM_001112718 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001112718.1, NP_001106189.1 |
RefSeq Size | 6101 bp |
RefSeq ORF | 3171 bp |
Locus ID | 22998 |
UniProt ID | Q9UPQ0 |
Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226312 | LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 3 |
CNY 10,550.00 |
|
RC226312L3 | Lenti-ORF clone of LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 3 |
CNY 12,450.00 |
|
RC226312L4 | Lenti-ORF clone of LIMCH1 (mGFP-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 3 |
CNY 12,450.00 |
|
RG226312 | LIMCH1 (tGFP-tagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 3 |
CNY 11,590.00 |