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ARHGEF14 (MCF2L) (NM_024979) Human Untagged Clone
CAT＃: SC318509
MCF2L (untagged)-Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 2
CNY 21,760.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARHGEF14; DBS; OST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318509 representing NM_024979.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACGGTGCGCCGGCTGTCACTGCTGTGCCGGGACCTCTGGGCGCTGTGGCTGCTGCTGAAGGCCGGC GCAGATGAAATCATGCACCAGGACATCGTCCCGCTCTGTGCTGCCGACATCCAGGACCAGCTAAAGAAG CGCTTTGCTTACCTGTCCGGTGGGCGGGGGCAGGACGGAAGCCCGGTTATCACCTTCCCTGACTACCCG GCCTTCAGCGAGATTCCGGACAAGGAGTTCCAGAATGTCATGACCTACCTCACCAGCATCCCCAGCCTG CAGGACGCTGGCATCGGATTCATCCTGGTGATAGACCGGCGACGGGACAAATGGACCTCCGTGAAGGCG TCCGTCCTGCGCATCGCAGCATCTTTCCCGGCAAACCTGCAGCTCGTCCTCGTGCTTCGCCCGACGGGT TTTTTCCAAAGGACTCTCTCCGACATCGCTTTCAAATTCAATAGAGATGACTTTAAGATGAAGGTGCCG GTCATAATGCTGAGCTCCGTACCAGACTTACACGGTTACATCGATAAGTCGCAGCTGACCGAGGACCTG GGTGGGACCCTGGACTACTGCCACTCCCGGTGGCTGTGCCAGCGCACGGCCATCGAAAGTTTCGCCCTC ATGGTGAAGCAGACGGCTCAGATGCTGCAGTCCTTCGGGACCGAGCTGGCTGAAACAGAGCTGCCCAAT GACGTCCAGTCGACAAGCTCAGTGCTGTGTGCGCACACAGAGAAGAAGGACAAGGCGAAGGAGGATTTG AGGCTGGCACTGAAAGAGGGGCACAGTGTCCTGGAGAGCCTCAGGGAGCTGCAGGCTGAGGGCTCAGAG CCCAGTGTGAACCAGGACCAGCTTGACAACCAGGCCACCGTGCAGAGGCTCCTGGCCCAGCTGAACGAA ACCGAGGCTGCCTTCGATGAGTTCTGGGCAAAGCATCAGCAGAAACTGGAGCAGTGTCTGCAGCTCCGG CACTTTGAGCAGGGCTTCCGGGAGGTCAAAGCCATCTTGGACGCAGCGTCCCAGAAGATAGCAACCTTC ACAGACATCGGCAACAGCCTGGCGCATGTGGAGCACCTGCTGAGGGACCTGGCCAGCTTCGAGGAGAAA TCAGGCGTGGCCGTGGAGAGGGCCCGGGCCCTGTCTCTGGACGGCGAGCAGCTCATTGGGAACAAGCAC TACGCGGTAGACTCCATCCGCCCAAAGTGCCAGGAGCTCCGGCACCTCTGTGACCAGTTCTCTGCGGAG ATCGCAAGGAGGAGGGGGCTGCTCAGCAAGTCCCTGGAGCTGCACCGCCGCCTGGAGACGTCCATGAAG TGGTGTGATGAAGGGATTTACCTGCTGGCCTCACAACCTGTGGACAAGTGCCAGTCCCAGGACGGCGCG GAGGCTGCCCTCCAGGAAATCGAGAAGTTTTTGGAGACCGGTGCGGAAAATAAGATCCAGGAGCTCAAC GCGATTTACAAGGAATACGAATCCATCCTCAACCAAGATCTCATGGAGCACGTGCGAAAGGTCTTCCAG AAGCAGGCAAGCATGGAGGAGGTGTTCCACCGCAGGCAGGCCAGCCTGAAGAAGCTGGCGGCCAGGCAG ACGCGGCCCGTGCAGCCGGTGGCCCCCAGACCCGAGGCACTGGCAAAGTCGCCCTGCCCCTCCCCAGGC ATTCGGCGAGGCTCTGAGAACTCCAGCTCCGAGGGCGGTGCGCTCCGGAGAGGGCCCTACCGGAGGGCC AAGAGTGAGATGAGTGAGAGCCGGCAGGGCCGCGGCTCAGCGGGGGAGGAGGAGGAAAGCCTGGCCATC CTGCGCAGGCACGTGATGAGCGAGCTCCTGGACACAGAACGGGCCTACGTGGAGGAGCTGCTGTGCGTC CTGGAGGGCTACGCCGCGGAGATGGATAACCCACTGATGGCTCACCTCCTGTCAACAGGCCTTCACAAC AAGAAGGATGTTTTGTTTGGAAACATGGAGGAAATCTATCACTTCCACAACAGGATATTCCTCAGGGAG CTGGAAAACTACACTGACTGCCCAGAACTGGTTGGAAGATGCTTTCTGGAGAGGATGGAAGATTTCCAG ATCTATGAGAAGTACTGTCAGAACAAGCCCCGCTCTGAGAGCCTGTGGAGACAGTGCTCCGACTGCCCG TTTTTCCAGGAATGCCAGAGAAAGCTGGACCACAAGCTGAGCCTGGACTCCTACCTGCTGAAGCCAGTG CAGAGGATCACCAAGTACCAGCTGCTGCTCAAGGAAATGCTGAAATACAGCAGGAACTGCGAGGGGGCT GAGGACCTGCAGGAGGCGCTGAGCTCCATCCTGGGCATCCTGAAGGCCGTGAACGACTCCATGCACCTC ATCGCTATCACCGGCTATGACGGGAATCTCGGCGACCTGGGCAAGCTGCTGATGCAGGGCTCGTTCAGC GTCTGGACCGACCACAAGAGGGGCCACACCAAGGTGAAGGAGCTGGCCAGGTTCAAGCCCATGCAGCGG CACCTGTTCCTGCACGAGAAGGCAGTGCTCTTCTGCAAGAAGAGGGAGGAGAATGGGGAGGGGTATGAG AAAGCTCCCTCCTACAGCTACAAGCAGTCCTTAAACATGGCTGCCGTTGGCATTACGGAGAACGTGAAG GGAGATGCTAAGAAGTTCGAGATCTGGTACAACGCGCGCGAGGAGGTCTACATCGTCCAGGCGCCAACT CCTGAGATTAAAGCCGCGTGGGTGAATGAAATTCGGAAAGTGCTGACCAGCCAGCTGCAGGCTTGTAGA GAAGCCAGCCAGCACCGGGCGCTGGAGCAGTCACAGAGCCTGCCCCTGCCGGCCCCGACCAGCACCAGT CCCTCAAGAGGAAACTCAAGGAACATCAAGAAGCTGGAAGAAAGGAAAACAGACCCCCTAAGCCTGGAG GGATACGTCAGCTCAGCGCCACTGACAAAGCCCCCCGAAAAGGGCAAAGGTTGGAGCAAAACGTCCCAC TCACTGGAGGCACCTGAGGACGACGGGGGCTGGTCAAGTGCAGAGGAGCAGATTAACTCGTCCGACGCA GAGGAGGACGGCGGGTTGGGCCCCAAGAAGCTGGTTCCAGGTAAATACACGGTCGTGGCGGACCACGAG AAGGGAGGCCCCGATGCGCTGCGCGTGAGGAGCGGGGACGTGGTGGAGCTGGTGCAGGAGGGCGACGAG GGCCTCTGGTACGTCAGGGACCCGACCACTGGCAAGGAGGGCTGGGTGCCGGCCAGCAGCCTGTCCGTC CGGCTCGGCCCGTCCGGCTCGGCCCAGTGCCTGAGCAGCTCAGAGTCGAGCCCGGGGTCGGCCGTGCTG AGCAACTCGTCCAGCTGCAGCGAGGGCGGCCAGGCCCCCTTCTCCGACCTGCAGGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024979 |
| Insert Size | 3372 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024979.4 |
| RefSeq Size | 6456 bp |
| RefSeq ORF | 3372 bp |
| Locus ID | 23263 |
| UniProt ID | O15068 |
| MW | 126.1 kDa |
| Gene Summary | This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1, resulting in an isoform (b) with a distinct and shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. CCDS Note: The coding region has been updated to represent an alternative splicing pattern that is more supported by the available transcript data. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205825 | MCF2L (Myc-DDK-tagged)-Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 2 |
CNY 7,216.00 |
|
| RC205825L3 | Lenti ORF clone of Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 2, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC205825L4 | Lenti ORF clone of Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 2, mGFP tagged |
CNY 10,260.00 |
|
| RG205825 | MCF2L (tGFP-tagged) - Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 2 |
CNY 9,220.00 |
|
| SC122978 | MCF2L (untagged)-Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 2 |
CNY 7,216.00 |
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