ASAP3 (NM_017707) Human Untagged Clone

CAT#: SC318375

ASAP3 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1



  "NM_017707" in other vectors (5)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal Uplc1/Asap3 antibody
    • 100 ug

CNY 8,025.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACAP4; CENTB6; DDEFL1; UPLC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC318375 representing NM_017707.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCGGAGCAGTTCAGCGTCGCCGAGTTCCTGGCCGTCACCGCGGAGGACCTCAGCTCCCCGGCTGGG
GCCGCCGCCTTCGCCGCCAAGATGCCCCGGTACCGAGGGGCGGCGCTGGCGCGGGAGGAGATCTTGGAA
GGAGACCAAGCCATCCTGCAGAGAATAAAGAAGGCTGTGCGGGCAATCCATAGCTCCGGCCTTGGCCAT
GTGGAGAATGAAGAGCAGTACCGAGAGGCCGTGGAATCCTTAGGCAACAGCCACCTGTCCCAGAACAGC
CATGAGCTGTCCACAGGCTTCCTAAACTTGGCCGTGTTCACCCGCGAGGTTGCTGCGCTCTTCAAGAAC
CTGATTCAGAACTTGAACAACATTGTCTCTTTCCCCCTGGACAGTCTGATGAAGGGGCAGCTGAGGGAC
GGTCGACAGGATTCCAAAAAACAGCTGGAGAAGGCATGGAAGGACTATGAAGCCAAAATGGCCAAGCTG
GAGAAGGAGCGCGATCGGGCCAGGGTGACAGGAGGGATCCCTGGGGAGGTGGCCCAGGACATGCAGAGA
GAGCGGCGCATCTTCCAGCTGCACATGTGTGAGTATCTGCTCAAAGCCGGGGAGAGCCAGATGAAGCAA
GGTCCTGACTTCCTTCAGAGCCTCATCAAGTTCTTCCACGCCCAGCACAACTTTTTCCAAGATGGCTGG
AAGGCTGCCCAGAGCCTGTTCCCCTTCATCGAGAAGCTGGCGGCCTCAGTACATGCACTCCATCAGGCC
CAGGAGGACGAGCTACAGAAGCTGACCCAGCTCCGGGACTCCCTCCGAGGGACACTGCAGCTTGAGAGC
AGAGAGGAACACCTGAGCCGGAAGAACTCAGGATGTGGCTATAGCATCCACCAGCACCAAGGCAACAAG
CAGTTTGGGACGGAGAAAGTGGGCTTTCTATACAAGAAAAGTGACGGAATTCGAAGAGTCTGGCAGAAA
AGGAAGTGTGGAGTCAAGTATGGCTGCCTGACCATCTCACACAGCACGATAAACCGGCCCCCGGTGAAG
CTGACCCTGCTGACGTGCCAAGTGAGGCCAAACCCTGAGGAGAAAAAGTGCTTCGACCTGGTGACCCAC
AACCGGACGTACCACTTTCAGGCAGAGGACGAGCACGAGTGTGAGGCGTGGGTGTCAGTGTTGCAGAAC
AGCAAGGACGAAGCCCTGAGCAGCGCCTTCCTCGGGGAGCCCAGCGCTGGCCCGGGGTCCTGGGGGTCC
GCCGGCCATGATGGGGAGCCGCACGACCTCACAAAGCTGCTCATCGCGGAGGTGAAGAGCAGGCCTGGG
AATAGCCAGTGCTGCGACTGCGGGGCTGCAGACCCCACGTGGCTCAGCACCAACCTGGGCGTGCTCACC
TGCATCCAGTGCTCGGGCGTCCACCGCGAACTGGGCGTGCGCTTTTCGCGCATGCAGTCACTCACCTTG
GACCTGCTGGGCCCCTCCGAGTTGTTGCTGGCCTTGAACATGGGAAACACGAGCTTCAATGAGGTCATG
GAGGCCCAGCTACCCTCACACGGCGGCCCTAAACCCTCAGCTGAGAGTGACATGGGCACCCGCAGGGAC
TACATTATGGCCAAGTATGTGGAGCATAGGTTTGCACGCCGGTGCACACCTGAGCCTCAGCGACTCTGG
ACAGCCATTTGCAACAGGGACCTCCTGTCGGTACTGGAGGCCTTTGCCAATGGGCAGGACTTTGGACAG
CCGCTGCCAGGGCCTGATGCACAGGCACCTGAAGAACTCGTCTTGCATTTGGCTGTCAAAGTCGCCAAC
CAGGCTTCCCTGCCTCTGGTGGATTTCATCATCCAGAACGGTGGTCACCTGGATGCCAAGGCTGCTGAC
GGGAACACGGCTCTGCACTACGCAGCACTCTACAACCAGCCCGACTGCCTCAAGCTGCTGCTGAAGGGG
AGAGCTTTGGTTGGCACAGTAAATGAAGCAGGCGAGACAGCTCTGGACATAGCCAGGAAGAAGCACCAC
AAGGAGTGTGAGGAGCTGCTGGAGCAGGCCCAGGCGGGGACCTTTGCCTTCCCTCTACATGTGGACTAC
TCCTGGGTAATTTCCACAGAGCCTGGCTCTGACAGTGAGGAGGATGAGGAAGAGAAGCGCTGCTTGCTG
AAGCTCCCGGCCCAGGCTCACTGGGCCAGTGGGAGGCTGGACATCAGCAACAAGACCTATGAGACTGTC
GCCAGCCTGGGAGCAGCCACCCCTCAGGGCGAGAGTGAGGACTGTCCCCCGCCCTTGCCAGTCAAAAAC
TCTTCTCGGACTTTGGTCCAAGGGTGTGCAAGACATGCCAGTGGAGATCGTTCTGAAGTCTCCAGCCTG
AGTTCAGAGGCCCCTGAGACCCCTGAGAGCCTGGGCAGTCCAGCCTCCTCCTCCAGTCTGATGAGCCCC
TTGGAACCTGGGGATCCCAGCCAAGCCCCACCCAACTCTGAAGAGGGCCTCCGAGAGCCCCCAGGCACC
TCCAGACCCAGCCTGACATCCGGGACCACCCCTTCGGAGATGTACCTCCCCGTCAGATTCAGCTCCGAG
AGCACTCGCTCCTATCGGCGGGGGGCGCGGAGCCCTGAAGATGGTCCCTCAGCCAGGCAGCCTCTGCCC
AGAAGGAACGTGCCGGTTGGCATCACTGAAGGAGATGGCTCAAGGACTGGGAGTCTCCCAGCAAGTTCT
GTGCAACTTTTGCAAGACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_017707
Insert Size 2712 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017707.3
RefSeq Size 4199 bp
RefSeq ORF 2712 bp
Locus ID 55616
UniProt ID Q8TDY4
Domains ArfGap, PH, ANK
Protein Pathways Endocytosis, Fc gamma R-mediated phagocytosis
MW 99.2 kDa
Gene Summary This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
Transcript Variant: This variant (1) encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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