United States
Germany
Japan
United Kingdom
China
LRRC8D (NM_018103) Human Untagged Clone
CAT＃: SC318336
LRRC8D (untagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LRRC5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318336 representing NM_018103.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTACCCTTGCGGAAGTTGCATCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAACCATGG TGGGATGTGTTTATGGATTACCTAGCTGTTGTTATGTTAATGGTAGCCATCTTTGCAGGAACCATGCAA CTTACCAAAGATCAGGTGGTCTGTTTGCCAGTATTGCCATCTCCTGTAAATTCAAAGGCACATACACCA CCAGGAAATGCCGAGGTCACCACCAACATCCCAAAGATGGAAGCAGCCACCAACCAAGACCAAGATGGG CGGACAACAAACGACATTTCCTTTGGGACATCTGCTGTGACACCTGACATACCTCTCAGAGCCACATAT CCTCGCACAGATTTCGCACTTCCAAATCAGGAGGCAAAGAAAGAGAAGAAAGATCCAACAGGTCGAAAA ACAAACTTGGATTTTCAGCAATATGTATTTATTAATCAAATGTGTTACCATCTGGCCCTTCCGTGGTAT TCTAAGTACTTTCCATACCTAGCTCTTATACATACTATTATTCTCATGGTCAGTAGCAACTTTTGGTTC AAATATCCCAAAACATGCTCAAAAGTAGAACATTTTGTTTCAATATTAGGAAAGTGCTTTGAATCCCCT TGGACGACAAAAGCGTTGTCTGAGACAGCATGCGAAGACTCAGAGGAAAACAAGCAGAGAATAACAGGT GCCCAGACTCTACCAAAGCATGTTTCTACCAGCAGTGATGAAGGGAGCCCCAGTGCCAGTACACCAATG ATCAATAAAACTGGCTTTAAATTTTCAGCTGAGAAGCCTGTGATTGAAGTTCCCAGCATGACAATCCTG GATAAAAAGGATGGAGAGCAGGCCAAAGCCCTGTTTGAGAAAGTGAGGAAGTTCCGTGCCCATGTGGAA GATAGTGACTTGATCTATAAACTCTATGTGGTCCAAACAGTTATCAAAACAGCCAAGTTCATTTTTATT CTCTGCTATACAGCGAACTTTGTCAACGCAATCAGCTTTGAACACGTCTGCAAGCCCAAAGTTGAGCAT CTGATTGGTTATGAGGTATTTGAGTGCACCCACAATATGGCTTACATGTTGAAAAAGCTTCTCATCAGT TACATATCCATTATTTGTGTTTATGGCTTTATCTGCCTCTACACTCTCTTCTGGTTATTCAGGATACCT TTGAAGGAATATTCTTTCGAAAAAGTCAGAGAAGAGAGCAGTTTTAGTGACATTCCAGATGTCAAAAAC GATTTTGCGTTCCTTCTTCACATGGTAGACCAGTATGACCAGCTATATTCCAAGCGTTTTGGTGTGTTC TTGTCAGAAGTTAGTGAAAATAAACTTAGGGAAATTAGTTTGAACCATGAGTGGACATTTGAAAAACTC AGGCAGCACATTTCACGCAACGCCCAGGACAAGCAGGAGTTGCATCTGTTCATGCTGTCGGGGGTGCCC GATGCTGTCTTTGACCTCACAGACCTGGATGTGCTAAAGCTTGAACTAATTCCAGAAGCTAAAATTCCT GCTAAGATTTCTCAAATGACTAACCTCCAAGAGCTCCACCTCTGCCACTGCCCTGCAAAAGTTGAACAG ACTGCTTTTAGCTTTCTTCGCGATCACTTGAGATGCCTTCACGTGAAGTTCACTGATGTGGCTGAAATT CCTGCCTGGGTGTATTTGCTCAAAAACCTTCGAGAGTTGTACTTAATAGGCAATTTGAACTCTGAAAAC AATAAGATGATAGGACTTGAATCTCTCCGAGAGTTGCGGCACCTTAAGATTCTCCACGTGAAGAGCAAT TTGACCAAAGTTCCCTCCAACATTACAGATGTGGCTCCACATCTTACAAAGTTAGTCATTCATAATGAC GGCACTAAACTCTTGGTACTGAACAGCCTTAAGAAAATGATGAATGTCGCTGAGCTGGAACTCCAGAAC TGTGAGCTAGAGAGAATCCCACATGCTATTTTCAGCCTCTCTAATTTACAGGAACTGGATTTAAAGTCC AATAACATTCGCACAATTGAGGAAATCATCAGTTTCCAGCATTTAAAACGACTGACTTGTTTAAAATTA TGGCATAACAAAATTGTTACTATTCCTCCCTCTATTACCCATGTCAAAAACTTGGAGTCACTTTATTTC TCTAACAACAAGCTCGAATCCTTACCAGTGGCAGTATTTAGTTTACAGAAACTCAGATGCTTAGATGTG AGCTACAACAACATTTCAATGATTCCAATAGAAATAGGATTGCTTCAGAACCTGCAGCATTTGCATATC ACTGGGAACAAAGTGGACATTCTGCCAAAACAATTGTTTAAATGCATAAAGTTGAGGACTTTGAATCTG GGACAGAACTGCATCACCTCACTCCCAGAGAAAGTTGGTCAGCTCTCCCAGCTCACTCAGCTGGAGCTG AAGGGGAACTGCTTGGACCGCCTGCCAGCCCAGCTGGGCCAGTGTCGGATGCTCAAGAAAAGCGGGCTT GTTGTGGAAGATCACCTTTTTGATACCCTGCCACTCGAAGTCAAAGAGGCATTGAATCAAGACATAAAT ATTCCCTTTGCAAATGGGATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_018103 |
| Insert Size | 2577 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018103.4 |
| RefSeq Size | 3782 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 55144 |
| UniProt ID | Q7L1W4 |
| Domains | LRR, LRR_TYP, LRR_BAC |
| Protein Families | Transmembrane |
| MW | 98.2 kDa |
| Gene Summary | Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24790029, PubMed:26824658, PubMed:28193731). Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed:28193731). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24782309, PubMed:24790029, PubMed:26824658, PubMed:28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Mediates the import of the antibiotic blasticidin-S into the cell (PubMed:24782309).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses a different segment for part of its 5' UTR, compared to variant 1. Variants 1 and 2 both encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203641 | LRRC8D (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2 |
CNY 6,288.00 |
|
| RC203641L1 | Lenti ORF clone of Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2, Myc-DDK-tagged |
CNY 8,688.00 |
|
| RC203641L2 | Lenti ORF clone of Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC203641L3 | Lenti ORF clone of Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203641L4 | Lenti ORF clone of Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG203641 | LRRC8D (tGFP-tagged) - Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2 |
CNY 4,370.00 |
|
| SC319580 | LRRC8D (untagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2 |
CNY 6,288.00 |
