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DARS2 (NM_018122) Human Untagged Clone
CAT＃: SC318102
DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASPRS; LBSL; MT-ASPRS; mtAspRS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318102 representing NM_018122.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTACTTCCCTTCTTGGTTAAGTCAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCACCCAA CCGATCTGGGGTTCTCTCTACAGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGC TTTGTTGTCCGGACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGT GGATGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTTCAAGTT ATCATTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTG GTGCAAGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAGAATCCAAAAATGCCAACAGGTGAG ATTGAAATCAAAGTTAAAACAGCTGAGCTTCTGAATGCCTGCAAGAAGCTGCCCTTTGAAATTAAGAAC TTCGTGAAGAAAACAGAGGCTCTTCGGTTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAG TATAACCTGCGACTGAGGTCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTT GTGGATATAGAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCC AGGGAACCTGGAAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTGATGGTTGGC GGTTTAGACAGATATTTTCAGGTTGCCCGATGTTATCGAGATGAAGGTTCAAGACCAGACAGACAGCCT GAGTTTACTCAGATTGACATAGAGATGTCATTTGTAGACCAGACTGGGATCCAGAGTTTAATTGAGGGT TTGCTCCAGTATTCCTGGCCCAATGACAAAGATCCTGTGGTTGTTCCTTTTCCTACTATGACTTTTGCT GAGGTGCTGGCCACCTATGGAACTGATAAACCTGACACTCGCTTTGGAATGAAGATTATAGATATCAGT GATGTGTTTAGAAACACAGAGATTGGATTTCTTCAAGATGCACTTAGTAAGCCCCATGGAACTGTGAAA GCCATATGTATCCCTGAAGGAGCAAAATACTTAAAAAGGAAAGACATTGAATCCATTAGAAACTTTGCA GCTGACCATTTTAATCAGGAAATCTTACCTGTATTCCTTAACGCCAATAGAAACTGGAATTCTCCAGTT GCTAATTTCATAATGGAGTCACAAAGACTGGAATTAATCAGACTAATGGAGACCCAAGAGGAAGATGTG GTCCTACTAACTGCTGGAGAGCACAATAAAGCATGCTCTTTGTTAGGAAAATTACGACTGGAATGTGCT GACCTTCTAGAAACAAGAGGAGTGGTGCTCCGTGACCCCACTCTGTTCTCTTTCCTTTGGGTGGTAGAT TTCCCACTCTTCCTGCCCAAGGAGGAAAATCCCAGAGAGCTGGAATCGGCCCACCACCCATTTACTGCT CCCCACCCCAGTGACATACATCTCCTGTACACTGAGCCCAAAAAGGCCCGTAGCCAACACTATGACTTG GTTTTAAATGGCAATGAAATAGGAGGTGGTTCAATTCGAATTCACAATGCAGAGCTGCAGCGTTATATC CTGGCAACCTTACTAAAGGAGGATGTGAAAATGCTCTCCCATCTGCTCCAGGCTTTAGATTATGGGGCA CCCCCTCATGGAGGAATTGCCTTAGGGTTAGACAGACTGATATGCCTTGTCACTGGATCTCCAAGCATC AGAGATGTCATAGCCTTCCCAAAGTCCTTCCGGGGACATGACCTCATGAGCAATACCCCAGATTCTGTC CCTCCTGAGGAACTGAAGCCCTATCATATCCGAGTCTCCAAGCCAACAGACTCCAAAGCAGAAAGAGCT CATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_018122 |
| Insert Size | 1938 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018122.4 |
| RefSeq Size | 3348 bp |
| RefSeq ORF | 1938 bp |
| Locus ID | 55157 |
| UniProt ID | Q6PI48 |
| Domains | GAD, tRNA-synt_2 |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| MW | 73.6 kDa |
| Gene Summary | The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207360 | DARS2 (Myc-DDK-tagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein |
CNY 4,800.00 |
|
| RC207360L1 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,200.00 |
|
| RC207360L2 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
| RC207360L3 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207360L4 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
| RG207360 | DARS2 (tGFP-tagged) - Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein |
CNY 6,400.00 |
|
| SC113685 | DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein |
CNY 7,768.00 |
