ZFP90 (NM_133458) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FIK; NK10; zfp-90; ZNF756
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC318088 sequence for NM_133458 edited (data generated by NextGen Sequencing) ATGGCCCCGAGGCCTCCGACCGCCGCGCCCCAGGAATCAGTGACATTCAAAGATGTGTCTGTGGACTTCA CCCAGGAAGAATGGTACCATGTCGACCCTGCTCAGAGGAGCTTATACAGGGATGTGATGCTGGAGAACTA TAGCCACCTGGTTTCTCTTGGATATCAAGTTTCCAAGCCAGAGGTGATCTTCAAATTGGAGCAAGGAGAA GAGCCATGGATATCAGAGGGAGAAATCCAACGACCTTTCTATCCAGACTGGAAGACCAGGCCTGAAGTCA AATCATCACATTTGCAGCAGGATGTATCAGAAGTATCCCACTGCACACATGATCTCTTACATGCTACATT AGAAGACTCCTGGGATGTTAGCAGCCAGTTAGACAGGCAACAGGAAAACTGGAAGAGACATCTGGGATCA GAGGCATCCACCCAGAAGAAAATAATTACACCACAAGAAAATTTTGAGCAAAATAAATTTGGTGAAAATT CTAGATTGAACACCAATTTGGTTACACAACTGAACATTCCTGCAAGAATAAGGCCTAGTGAATGTGAGAC CCTTGGAAGCAATTTGGGACATAATGCAGACTTACTTAATGAGAATAATATTCTTGCAAAAAAGAAACCC TATAAGTGTGATAAATGTAGAAAAGCCTTTATTCATAGATCATCGCTTACTAAACATGAGAAAACACATA AAGGAGAGGGAGCTTTCCCTAATGGAACAGATCAAGGAATTTATCCTGGAAAGAAACACCATGAATGTAC CGACTGTGGGAAAACCTTTCTCTGGAAGACACAGCTTACTGAGCATCAGAGAATTCACACTGGGGAGAAA CCCTTTGAATGCAATGTATGTGGAAAGGCCTTCAGGCATAGCTCATCTCTTGGTCAGCATGAGAATGCTC ATACCGGAGAGAAACCCTATCAGTGTAGTCTCTGTGGGAAAGCCTTCCAGCGCAGCTCCTCCCTTGTTCA ACACCAGCGAATTCACACTGGAGAGAAACCCTATCGATGTAATCTATGTGGGAGGTCCTTTAGGCATGGC ACATCCCTCACTCAACACGAGGTCACACACAGTGGAGAGAAGCCCTTCCAGTGTAAGGAATGTGGGAAAG CCTTTAGTCGATGTTCTTCCCTTGTCCAACATGAGAGGACTCATACTGGAGAGAAACCTTTTGAATGTAG CATATGTGGGAGGGCTTTTGGTCAGAGCCCATCCCTTTATAAACATATGAGGATTCATAAGAGAGGCAAA CCTTACCAAAGCAGTAACTACAGCATAGATTTCAAGCACAGCACATCTCTCACTCAGGATGAAAGCACTC TTACCGAAGTGAAATCCTACCATTGTAATGACTGTGGGGAAGACTTTAGTCACATTACAGACTTTACTGA CCATCAGAGGATCCATACTGCAGAGAACCCCTATGATTGTGAGCAGGCTTTTAGTCAGCAAGCTATTTCT CATCCTGGAGAGAAACCCTATCAATGTAATGTATGTGGGAAAGCTTTCAAAAGGAGTACAAGTTTCATAG AGCATCACAGAATTCATACTGGAGAGAAACCCTATGAATGTAATGAGTGTGGAGAAGCCTTTAGTCGACG CTCATCGCTTACTCAACATGAGAGAACCCACACTGGAGAGAAACCCTATGAATGTATTGACTGTGGGAAA GCCTTTAGTCAAAGTTCATCTCTCATTCAGCATGAGAGAACTCATACTGGAGAGAAGCCCTATGAATGTA ATGAATGTGGGAGAGCCTTCCGAAAAAAAACCAACCTGCATGATCATCAGAGAATTCATACTGGAGAAAA ACCCTATTCTTGTAAGGAATGTGGGAAAAACTTCAGCCGAAGTTCAGCTCTTACTAAACACCAGAGAATT CATACTCGAAATAAACTCTAG Clone variation with respect to NM_133458.3 1317:g=>a
Restriction Sites	Please inquire
ACCN	NM_133458
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133458.2, NP_597715.2
RefSeq Size	4655 bp
RefSeq ORF	1911 bp
Locus ID	146198
UniProt ID	Q8TF47
Gene Summary	This gene encodes a member of the zinc finger protein family that modulates gene expression. The encoded protein derepresses the transcription of certain fetal cardiac genes and may contribute to the genetic reprogramming that occurs during the development of heart failure. Genome wide association studies have identified this gene among ulcerative colitis risk loci. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015] Transcript Variant: This variant (1) encodes the longest protein. Both variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.