IDI1 (NM_004508) Human Untagged Clone
CAT#: SC317411
IDI1 (untagged)-Human isopentenyl-diphosphate delta isomerase 1 (IDI1)
CNY 6,270.00
Product images
CNY 6,281.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | IPP1; IPPI1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC317411 representing NM_004508.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGCGTGGACTGGCGCTGGCGCGAGCGATTGGCTGCGCGGCCCGGGGGCGGGGCCAGTGGGCGGTG CGCGCCGCAGACTGTGCTCAAAGCGGGCGCCATCCGGGACCGGCGGTTGTCTGTGGCCGGAGGCTGATC AGTGTTCTAGAACAGATCAGACATTTTGTAATGATGCCTGAAATAAACACTAACCACCTCGACAAGCAA CAGGTTCAACTCCTGGCAGAGATGTGTATCCTTATTGATGAAAATGACAATAAAATTGGAGCTGAGACC AAGAAGAATTGTCACCTGAACGAGAACATTGAGAAAGGATTATTGCATCGAGCTTTTAGTGTCTTCTTA TTCAACACCGAAAATAAGCTTCTGCTACAGCAAAGATCAGATGCTAAGATTACCTTTCCAGGTTGTTTT ACGAATACGTGTTGTAGTCATCCATTAAGCAATCCAGCCGAGCTTGAGGAAAGTGACGCCCTTGGAGTG AGGCGAGCAGCACAGAGACGGCTGAAAGCTGAGCTAGGAATTCCCTTGGAAGAGGTTCCTCCAGAAGAA ATTAATTATTTAACACGAATTCACTACAAAGCTCAGTCTGATGGTATCTGGGGTGAACATGAAATTGAT TACATTTTGTTGGTGAGGAAGAATGTAACTTTGAATCCAGATCCCAATGAGATTAAAAGCTATTGTTAT GTGTCAAAGGAAGAACTAAAAGAACTTCTGAAAAAAGCAGCCAGTGGTGAAATTAAGATAACGCCATGG TTTAAAATTATTGCAGCGACTTTTCTCTTTAAATGGTGGGATAACTTAAATCATTTGAATCAGTTTGTT GACCATGAGAAAATATACAGAATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_004508 |
Insert Size | 855 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004508.3 |
RefSeq Size | 2996 bp |
RefSeq ORF | 855 bp |
Locus ID | 3422 |
UniProt ID | Q13907 |
Domains | NUDIX |
Protein Pathways | Metabolic pathways, Terpenoid backbone biosynthesis |
MW | 32.5 kDa |
Gene Summary | IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (a). |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214341 | IDI1 (Myc-DDK-tagged)-Human isopentenyl-diphosphate delta isomerase 1 (IDI1) |
CNY 2,400.00 |
|
RC214341L1 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC214341L2 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), mGFP tagged |
CNY 5,890.00 |
|
RC214341L3 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC214341L4 | Lenti ORF clone of Human isopentenyl-diphosphate delta isomerase 1 (IDI1), mGFP tagged |
CNY 5,890.00 |
|
RG214341 | IDI1 (tGFP-tagged) - Human isopentenyl-diphosphate delta isomerase 1 (IDI1) |
CNY 4,370.00 |
|
SC117309 | IDI1 (untagged)-Human isopentenyl-diphosphate delta isomerase 1 (IDI1) |
CNY 2,400.00 |