Neurofilament (NEFM) (NM_001105541) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NEF3; NF-M; NFM
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001105541, the custom clone sequence may differ by one or more nucleotides ATGGCTCGTCATTTGCGCGAATACCAGGACCTCCTCAACGTCAAGATGGCTCTGGATATA GAAATCGCTGCGTACAGAAAACTCCTGGAGGGTGAAGAGACTAGATTTAGCACATTTGCA GGAAGCATCACTGGGCCACTGTATACACACCGACCCCCAATCACAATATCCAGTAAGATT CAGAAACCCAAGGTGGAAGCTCCCAAGCTTAAGGTCCAACACAAATTTGTCGAGGAGATC ATAGAGGAAACCAAAGTGGAGGATGAGAAGTCAGAAATGGAAGAGGCCCTGACAGCCATT ACAGAGGAATTGGCCGTTTCCATGAAGGAAGAGAAGAAAGAAGCAGCAGAAGAAAAGGAA GAGGAACCCGAAGCTGAAGAAGAAGAAGTAGCTGCCAAAAAGTCTCCAGTGAAAGCAACT GCACCTGAAGTTAAAGAAGAGGAAGGGGAAAAGGAGGAAGAAGAAGGCCAGGAAGAAGAG GAGGAAGAAGATGAGGGAGCTAAGTCAGACCAAGCCGAAGAGGGAGGATCCGAGAAGGAA GGCTCTAGTGAAAAAGAGGAAGGTGAGCAGGAAGAAGGAGAAACAGAAGCTGAAGCTGAA GGAGAGGAAGCCGAAGCTAAAGAGGAAAAGAAAGTGGAGGAAAAGAGTGAGGAAGTGGCT ACCAAGGAGGAGCTGGTGGCAGATGCCAAGGTGGAAAAGCCAGAAAAAGCCAAGTCTCCT GTGCCAAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAA GAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTG CCGAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGTCAAAATCACCAGTGGAAGAG AAAGCCAAATCTCCTGTGCCAAAATCACCAGTGGAAGAGGCAAAGTCAAAAGCAGAAGTG GGGAAAGGTGAACAGAAAGAGGAAGAAGAAAAGGAAGTCAAGGAAGCTCCCAAGGAAGAG AAGGTAGAGAAAAAGGAAGAGAAACCAAAGGATGTGCCAGAGAAGAAGAAAGCTGAGTCC CCTGTAAAGGAGGAAGCTGTGGCAGAGGTGGTCACCATCACCAAATCGGTAAAGGTGCAC TTGGAGAAAGAGACCAAAGAAGAGGGGAAGCCACTGCAGCAGGAGAAAGAGAAGGAGAAA GCGGGAGGAGAGGGAGGAAGTGAGGAGGAAGGGAGTGATAAAGGTGCCAAGGGATCCAGG AAGGAAGACATAGCTGTCAATGGGGAGGTAGAAGGAAAAGAGGAGGTAGAGCAGGAGACC AAGGAAAAAGGCAGTGGGAGGGAAGAGGAGAAAGGCGTTGTCACCAATGGCCTAGACTTG AGCCCAGCAGATGAAAAGAAGGGGGGTGATAAAAGTGAGGAGAAAGTGGTGGTGACCAAA ACGGTAGAAAAAATCACCAGTGAGGGGGGAGATGGTGCTACCAAATACATCACTAAATCT GTAACCGTCACTCAAAAGGTTGAAGAGCATGAAGAGACCTTTGAGGAGAAACTAGTGTCT ACTAAAAAGGTAGAAAAAGTCACTTCACACGCCATAGTAAAGGAAGTCACCCAGAGTGAC
Restriction Sites	Please inquire
ACCN	NM_001105541
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105541.1, NP_001099011.1
RefSeq Size	2514 bp
RefSeq ORF	1623 bp
Locus ID	4741
Protein Pathways	Amyotrophic lateral sclerosis (ALS)
Gene Summary	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1.

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