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MITF (NM_000248) Human Untagged Clone
CAT＃: SC317086
MITF (untagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 4
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe32; CMM8; COMMAD; MI; WS2; WS2A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000248 edited
GGGAGGGATAGTCTACCGTCTCTCACTGGATTGGTGCCACCTAAAACATTGTTATGCTGG AAATGCTAGAATATAATCACTATCAGGTGCAGACCCACCTCGAAAACCCCACCAAGTACC ACATACAGCAAGCCCAACGGCAGCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATA AACATGCCAACCAAGTCCTGAGCTTGCCATGTCCAAACCAGCCTGGCGATCATGTCATGC CACCGGTGCCGGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCTTAACTCCA ACTGTGAAAAAGAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAGAGCGAGTGCC CAGGCATGAACACACATTCACGAGCGTCCTGTATGCAGATGGATGATGTAATCGATGACA TCATTAGCCTAGAATCAAGTTATAATGAGGAAATCTTGGGCTTGATGGATCCTGCTTTGC AAATGGCAAATACGTTGCCTGTCTCGGGAAACTTGATTGATCTTTATGGAAACCAAGGTC TGCCCCCACCAGGCCTCACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAACATAAAAA GGGAGCTCACAGCGTGTATTTTTCCCACAGAGTCTGAAGCAAGAGCACTGGCCAAAGAGA GGCAGAAAAAGGACAATCACAACCTGATTGAACGAAGAAGAAGATTTAACATAAATGACC GCATTAAAGAACTAGGTACTTTGATTCCCAAGTCAAATGATCCAGACATGCGCTGGAACA AGGGAACCATCTTAAAAGCATCCGTGGACTATATCCGAAAGTTGCAACGAGAACAGCAAC GCGCAAAAGAACTTGAAAACCGACAGAAGAAACTGGAGCACGCCAACCGGCATTTGTTGC TCAGAATACAGGAACTTGAAATGCAGGCTCGAGCTCATGGACTTTCCCTTATTCCATCCA CGGGTCTCTGCTCTCCAGATTTGGTGAATCGGATCATCAAGCAAGAACCCGTTCTTGAGA ACTGCAGCCAAGACCTCCTTCAGCATCATGCAGACCTAACCTGTACAACAACTCTCGATC TCACGGATGGCACCATCACCTTCAACAACAACCTCGGAACTGGGACTGAGGCCAACCAAG CCTATAGTGTCCCCACAAAAATGGGATCCAAACTGGAAGACATCCTGATGGACGACACCC TTTCTCCCGTCGGTGTCACTGATCCACTCCTTTCCTCAGTGTCCCCCGGAGCTTCCAAAA CAAGCAGCCGGAGGAGCAGTATGAGCATGGAAGAGACGGAGCACACTTGTTAGCGAATCC TCCCTGCACTGCATTCGCACAAACTGCTTCCTTTCTTGATTCGTAGATTTAATAACTTAC CTGAAGGGGTTTTCTTGATAATTTTCCTTTAATATGAAATTTTTTTTCATGCTTTATCAA TAGCCCAGGATATATTTTATTTTTAGAATTTTGTGAAACAGACTTGTATATTCTATTTTA CAACTACAAATGCCTCCAAAGTATTGTACAAATAAGTGTGCAGTATCTGTGAACTGAATT CACCACAGACTTTAGCTTTCTGAGCAAGAGGATTTTGCGTCAGAGAAATGTCTGTCCATT TTTATTCAGGGGAAACTTGATTTGAGATTTTTATGCCTGTGACTTCCTTGGAAATCAAAT GTAAAGTTTAATTGAAAGAATGTAAAGCAACCAAAAAGAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000248 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000248.2, NP_000239.1 |
| RefSeq Size | 4490 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 4286 |
| UniProt ID | O75030 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Melanogenesis, Melanoma, Pathways in cancer |
| Gene Summary | The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (4) differs in the 5' UTR, the 5' coding region and uses an alternate, in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (4), also known as isoform MITF-M, has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209561 | MITF (Myc-DDK-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 4 |
CNY 3,656.00 |
|
| RC209561L1 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209561L2 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, mGFP tagged |
CNY 6,056.00 |
|
| RC209561L3 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209561L4 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, mGFP tagged |
CNY 6,056.00 |
|
| RG209561 | MITF (tGFP-tagged) - Human microphthalmia-associated transcription factor (MITF), transcript variant 4 |
CNY 4,370.00 |
