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CRPPA (NM_001101417) Human Untagged Clone
CAT＃: SC317085
ISPD (untagged)-Human isoprenoid synthase domain containing (ISPD), transcript variant 2
CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hISPD; ISPD; LGMDR20; MDDGA7; MDDGC7; Nip |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317085 representing NM_001101417.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGCCGGGCCGCCGGGCAGCGCCAGGCCGGCGGAGCCGGGTCCTTGCCTGAGTGGTCAGCGCGGC GCGGACCACACGGCTTCCGCCTCCCTGCAGAGCGTGGCCGGGACCGAGCCCGGGCGCCACCCGCAAGCC GTGGCAGCTGTGTTGCCTGCCGGGGGGTGCGGGGAGAGGATGGGGGTCCCCACCCCGAAGCAATTCTGC CCCATCCTGGAGAGGCCGCTCATCAGCTACACCCTACAGGCCCTGGAGAGAGTATGTTGGATAAAGGAC ATTGTTGTGGCAGTAACTGGAGAGAACATGGAAGTAATGAAAAGTATTATTCAGAAGTATCAGCATAAA CGCATCTCACTGGTCGAAGCTGGAGTGACCCGCCACAGGTCAATTTTCAATGGACTAAAAGCACTGGCA GAAGATCAGATCAACTCTAAACTCTCTAAGCCAGAAGTAGTGATTATCCATGATGCTGTGAGACCATTT GTTGAGGAAGGTGTCCTTCTTAAAGTTGTCACAGCTGCTAAGGAACACGGGTGTAGTGACTATGACTTG GAATTTGGAACTGAGTGTTTGCAATTGGCCCTAAAATACTGTTGCACTAAAGCCAAACTTGTAGAAGGA TCACCTGACCTCTGGAAGGTGACCTACAAACGAGATCTCTATGCGGCTGAATCGATTATTAAGGAGAGA ATTTCCCAAGAGATTTGTGTAGTTATGGATACAGAAGAAGATAACAAACATGTAGGTCATCTTCTTGAA GAAGTGCTGAAAAGTGAATTAAATCATGTAAAAGTCACATCTGAGGCTCTGGGTCATGCTGGCAGACAT CTTCAGCAAATCATCTTAGATCAATGCTACAATTTTGTTTGTGTGAATGTTACAACCTCTGATTTTCAA GAAACCCAGAAGTTACTGAGCATGCTTGAAGAGAGTAGTCTTTGCATTTTATATCCTGTTGTTGTTGTT TCAGTTCATTTTCTTGATTTTAAATTAGTACCTCCCAGTCAGAAAATGGAAAACCTAATGCAGATTAGA GAATTTGCAAAGGAAGTAAAAGAAAGAAATATTTTGTTATATGGGCTTCTCATATCTTACCCACAGGAT GATCAGAAGCTACAGGAGAGTTTAAGGCAAGGTGCTATCATTATTGCTTCATTAATCAAGGAAAGAAAT TCTGGACTCATTGGTCAGCTTCTGATAGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001101417 |
| Insert Size | 1206 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001101417.3 |
| RefSeq Size | 5374 bp |
| RefSeq ORF | 1206 bp |
| Locus ID | 729920 |
| UniProt ID | A4D126 |
| MW | 44.4 kDa |
| Gene Summary | This gene encodes a 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein. Mutations in this gene are the cause of Walker-Warburg syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The full extent of this transcript is supported by and orthologous data in mouse (AK051322.1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225626 | ISPD (Myc-DDK-tagged)-Human isoprenoid synthase domain containing (ISPD), transcript variant 2 |
CNY 3,656.00 |
|
| RC225626L3 | Lenti ORF clone of Human isoprenoid synthase domain containing (ISPD), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC225626L4 | Lenti ORF clone of Human isoprenoid synthase domain containing (ISPD), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG225626 | ISPD (tGFP-tagged) - Human isoprenoid synthase domain containing (ISPD), transcript variant 2 |
CNY 4,370.00 |
