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KCNK10 (NM_138318) Human Untagged Clone
CAT＃: SC316623
KCNK10 (untagged)-Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3
CNY 4,048.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | K2p10.1; PPP1R97; TREK-2; TREK2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_138318, the custom clone sequence may differ by one or more nucleotides
ATGGAGGATGGATTTAAGGGGGACAGGACTGAAGGCTGTCGCAGTGATTCAGTGGCCGTTCCCGCAGCAG CACCGGTGTGCCAGCCCAAGAGCGCCACTAACGGGCAACCCCCGGCTCCGGCTCCGACTCCAACTCCGCG CCTGTCCATTTCCTCCCGAGCCACAGTGGTAGCCAGGATGGAAGGCACCTCCCAAGGGGGCTTGCAGACC GTCATGAAGTGGAAGACGGTGGTTGCCATCTTTGTGGTTGTGGTGGTCTACCTTGTCACTGGCGGTCTTG TCTTCCGGGCATTGGAGCAGCCCTTTGAGAGCAGCCAGAAGAATACCATCGCCTTGGAGAAGGCGGAATT CCTGCGGGATCATGTCTGTGTGAGCCCCCAGGAGCTGGAGACGTTGATCCAGCATGCTCTTGATGCTGAC AATGCGGGAGTCAGTCCAATAGGAAACTCTTCCAACAACAGCAGCCACTGGGACCTCGGCAGTGCCTTTT TCTTTGCTGGAACTGTCATTACGACCATAGGGTATGGGAATATTGCTCCGAGCACTGAAGGAGGCAAAAT CTTTTGTATTTTATATGCCATCTTTGGAATTCCACTCTTTGGTTTCTTATTGGCTGGAATTGGAGACCAA CTTGGAACCATCTTTGGGAAAAGCATTGCAAGAGTGGAGAAGGTCTTTCGAAAAAAGCAAGTGAGTCAGA CCAAGATCCGGGTCATCTCAACCATCCTGTTCATCTTGGCCGGCTGCATTGTGTTTGTGACGATCCCTGC TGTCATCTTTAAGTACATCGAGGGCTGGACGGCCTTGGAGTCCATTTACTTTGTGGTGGTCACTCTGACC ACGGTGGGCTTTGGTGATTTTGTGGCAGGGGGAAACGCTGGCATCAATTATCGGGAGTGGTATAAGCCCC TAGTGTGGTTTTGGATCCTTGTTGGCCTTGCCTACTTTGCAGCTGTCCTCAGTATGATCGGAGATTGGCT ACGGGTTCTGTCCAAAAAGACAAAAGAAGAGGTGGGTGAAATCAAGGCCCATGCGGCAGAGTGGAAGGCC AATGTCACGGCTGAGTTCCGGGAGACACGGCGAAGGCTCAGCGTGGAGATCCACGATAAGCTGCAGCGGG CGGCCACCATCCGCAGCATGGAGCGCCGGCGGCTGGGCCTGGACCAGCGGGCCCACTCACTGGACATGCT GTCCCCCGAGAAGCGCTCTGTCTTTGCTGCCCTGGACACCGGCCGCTTCAAGGCCTCATCCCAGGAGAGC ATCAACAACCGGCCCAACAACCTGCGCCTGAAGGGGCCGGAGCAGCTGAACAAGCATGGGCAGGGTGCGT CCGAGGACAACATCATCAACAAGTTCGGGTCCACCTCCAGACTCACCAAGAGGAAAAACAAGGACCTCAA AAAGACCTTGCCCGAGGACGTTCAGAAAATCTACAAGACCTTCCGGAATTACTCCCTGGACGAGGAGAAG AAAGAGGAGGAGACGGAAAAGATGTGTAACTCAGACAACTCCAGCACAGCCATGCTGACGGACTGTATCC AGCAGCACGCTGAGTTGGAGAACGGAATGATACCCACGGACACCAAAGACCGGGAGCCGGAGAACAACTC ATTACTTGAAGACAGAAACTAA >OriGene 5' read for NM_138318 unedited
GTGCCTTGTATACGACTCCTATAGGGCGGCCGCGAATTCATGGAGGATGGATTTAAGGGG GACAGGACTGAAGGCTGTCGCAGTGATTCAGTGGCCGTTCCCGCAGCAGCACCGGTGTGC CAGCCCAAGAGCGCCACTAACGGGCAACCCCCGGCTCCGGCTCCGACTCCAACTCCGCGC CTGTCCATTTCCTCCCGAGCCACAGTGGTAGCCAGGATGGAAGGCACCTCCCAAGGGGGC TTGCAGACCGTCATGAAGTGGAAGACGGTGGTTGCCATCTTTGTGGTTGTGGTGGTCTAC CTTGTCACTGGCGGTCTTGTCTTCCGGGCATTGGAGCAGCCCTTTGAGAGCAGCCAGAAG AATACCATCGCCTTGGAGAAGGCGGAATTCCTGCGGGATCATGTCTGTGTGAGCCCCCAG GAGCTGGAGACGTTGATCCAGCATGCTCTTGATGCTGACAATGCGGGAGTCAGTCCAATA GGAAACTCTTCCAACAACAGCAGCCACTGGGACCTCGGCAGTGCCTTTTTCTTTGCTGGA ACTGTCATTACGACCATAGGGTATGGGAATATTGCTCCGAGCACTGAAGGAGGCAAAATC TTTTGTATTTTATATGCCATCTTTGGAATTCCACTCTTTGGTTTCTTATTGGCTGGAATT GGAGACCAACTTGGAACCATCTTTGGGAAAAGCATTGCAAGAGTGGAGAAGTCTTTCGAA AAAAGCAAGTGAGTCAGACCAAGATCCCGGGTCATCTCAACCATCCTGTTCATCTTGGCC GGCTGCATTGTGTTTGTGACGATCCCTGCTGTCATCTTTAGTACATCGAGGGCTGACGCC TTGAGTCCATTTACTTTGTGTGTCACTCTGAACACGTGGGCTTTGTGATTTGTGCAGGGG AACGCTGGCATCATTATCGGGAGTGGTATTAAGCCCCCTAGA >OriGene 3' read for NM_138318 unedited
CGCTGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT TTTTTTTTTTTTGACATTTCCAAATCAGTTTATTGTCACCATTGCACCCACTGAGGAAAA TGCATGATTGGCCTTTAACCACGCAGCAGTTGATGTCATGTTTTCACCACTGCTCGCCAG TTCATGTGGACTACCTACCCCTGTGAAAAAAACCAAACTCAGCAGGCTTCTGTCATTGGT TAAGGTGATATCCACAGTGTGATGTGTTTGTGATTATTTTTTTTCTTTTGTCATGAAAAA AGCAATTGCACCTTTTCGTACGTGTGCTCTGATCTGCTTCCAACAAATTCCAGGTGCTCT CAGGTCCCCACAGACATTCATTTGGAGCATAAAAAGACTAGGTATGATGCACTGAATGGT TGGTATCAAAATTCAAGCTCAGAGCACATGCCAAAATGTCAACTCCAACGCTAGTTACAC ATAACCAGCTCCCACCCAATCAGGTCCCCTTCTGTGTGTGCACATATTACTACGACTCCA CTAAAAAGACACACCTGTTTCTTTCAGGAGGCTGGTCTAAGGAATAGCTGCCCTTGTCTA CGTGTGTGCCCAGGTAGCACTGCCCAGGGGTACAGCACTGCCACTGAAATGCCCTTAACA TGTACGCCAGAACCGGCTACGTGCACGGACACTCTTGGTGTGTCTGCGTTTGCTATCTGA AATGAAGTTCTTGTCTCTGATCCTTTGCAGAGCAGCTTTCAGTGTGATGGCACAGTGAAA TATATTCTCATGCTATGTATTGACTAAAAGTCTGTTTAGCACATGTCTCATGTGAATATT AAAACAC |
| Restriction Sites | Please inquire |
| ACCN | NM_138318 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138318.1, NP_612191.1 |
| RefSeq Size | 2401 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 54207 |
| UniProt ID | P57789 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Gene Summary | The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (3, also known as TREK2 splice variant b) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (3) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The isoforms generated by alternative translation initiation adopt similar conformation in the selectivity filter in TREK-2
,Zhuo, RG;Peng, P;Liu, XY;Zhang, SZ;Xu, JP;Zheng, JQ;Wei, XL;Ma, XY;,
J. Physiol. Biochem.
,PubMed ID 26271386
[KCNK10]
|
|
Insights into the stimulatory mechanism of 2-aminoethoxydiphenyl borate on TREK-2 potassium channel
,Zhuo, RG;Liu, XY;Zhang, SZ;Wei, XL;Zheng, JQ;Xu, JP;Ma, XY;,
Neuroscience
,PubMed ID 25982558
[KCNK10]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210446 | KCNK10 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3 |
CNY 4,040.00 |
|
| RC210446L3 | Lenti ORF clone of Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210446L4 | Lenti ORF clone of Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG210446 | KCNK10 (tGFP-tagged) - Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3 |
CNY 5,640.00 |
