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TAF4B (NM_005640) Human Untagged Clone
CAT＃: SC316429
TAF4B (untagged)-Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B)
CNY 16,630.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPGF13; TAF2C2; TAFII105 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316429 representing NM_005640.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCGCCGGCCTCACCGAACCCGCCGGCGCCGCTCCCCCGGCTGCTGTGAGCGCCTCGGGGACCGTG ACCATGGCCCCGGCCGGGGCGCTGCCGGTGCGGGTGGAGAGCACTCCGGTGGCCCTGGGCGCCGTGACT AAGGCTCCTGTCAGCGTCTGCGTGGAGCCCACGGCGTCCCAGCCCCTGCGGTCCCCCGTGGGGACCCTG GTGACCAAAGTGGCTCCGGTCAGCGCCCCTCCTAAAGTCAGCAGCGGCCCTAGGCTGCCTGCTCCTCAG ATAGTCGCCGTGAAAGCCCCCAACACCACGACAATCCAGTTTCCTGCTAATTTGCAGCTTCCTCCAGGA ACCGTTTTGATTAAAAGTAACAGTGGTCCGTTGATGTTGGTATCTCCTCAGCAAACTGTAACAAGAGCC GAGACCACAAGTAACATAACCTCAAGGCCAGCAGTACCAGCGAATCCTCAAACAGTCAAAATCTGTACA GTGCCGAACTCTAGCTCACAATTAATCAAGAAAGTGGCAGTGACACCTGTTAAAAAATTGGCACAAATA GGAACTACTGTGGTAACCACTGTTCCGAAGCCTTCCTCAGTACAATCTGTGGCTGTGCCAACCAGTGTC GTCACAGTTACTCCTGGAAAGCCATTGAATACTGTAACTACCCTGAAGCCTTCAAGTTTGGGAGCATCA TCCACTCCTTCAAATGAGCCCAATCTTAAAGCAGAGAACTCAGCAGCTGTTCAGATTAATCTTTCTCCG ACAATGCTAGAAAATGTGAAGAAATGCAAGAACTTCCTTGCAATGTTAATAAAACTAGCATGTAGTGGA TCACAGTCCCCAGAAATGGGGCAAAATGTGAAGAAGCTGGTGGAACAACTTTTGGATGCAAAAATCGAA GCAGAAGAATTTACTAGGAAACTGTATGTTGAACTCAAGTCTTCACCTCAGCCTCACCTGGTTCCTTTT CTTAAGAAAAGCGTGGTTGCCTTACGACAACTTCTGCCTAACTCCCAGAGCTTCATCCAGCAATGTGTT CAGCAGACTTCTAGTGACATGGTCATTGCTACCTGTACTACAACAGTAACAACTTCTCCTGTGGTGACA ACTACAGTGTCCTCAAGCCAGTCTGAAAAGTCAATTATTGTTTCTGGAGCAACAGCACCCAGAACTGTG TCAGTGCAAACTTTGAACCCACTTGCTGGTCCAGTGGGAGCAAAAGCTGGAGTTGTGACACTTCATTCT GTGGGCCCAACTGCTGCAACAGGAGGAACAACAGCTGGAACTGGTTTGCTTCAGACTTCAAAACCACTT GTGACATCTGTGGCAAACACAGTGACCACGGTCTCACTGCAACCTGAAAAGCCAGTTGTCTCTGGAACA GCAGTAACACTGTCCCTTCCAGCAGTAACTTTTGGAGAAACTTCAGGTGCAGCTATTTGTCTTCCATCT GTGAAACCTGTTGTTTCTTCTGCTGGGACCACATCTGACAAGCCTGTTATTGGGACTCCAGTTCAAATC AAACTTGCCCAGCCGGGCCCTGTCCTTTCACAACCAGCTGGGATTCCACAGGCAGTTCAAGTCAAGCAA CTAGTAGTTCAGCAGCCTTCAGGAGGCAATGAAAAACAAGTGACCACAATTTCACATTCCTCAACATTG ACCATTCAGAAATGTGGACAGAAGACGATGCCAGTGAACACCATAATACCTACTAGTCAGTTTCCTCCA GCTTCCATTCTAAAGCAAATTACTCTGCCTGGAAATAAAATTCTGTCACTTCAAGCATCTCCTACTCAG AAAAATAGAATAAAAGAGAATGTAACATCATGCTTCCGAGATGAGGATGACATCAATGATGTGACTTCT ATGGCAGGGGTCAACCTTAATGAAGAAAATGCCTGCATCTTAGCAACAAACTCTGAATTGGTTGGCACA CTCATTCAGTCATGTAAAGATGAACCATTTCTTTTTATTGGAGCTCTACAAAAGAGAATTTTAGACATT GGTAAAAAGCATGACATTACAGAACTTAACTCTGATGCTGTGAACTTGATCTCCCAAGCAACACAGGAA CGACTACGAGGCCTTCTAGAAAAACTGACTGCAATTGCTCAGCATCGAATGACTACTTACAAGGCAAGT GAAAATTACATCCTGTGTAGTGATACCAGGTCACAGCTCAAATTTCTTGAAAAGCTGGATCAATTGGAG AAGCAGAGAAAGGATTTGGAAGAAAGAGAAATGTTACTTAAGGCAGCCAAGAGTCGTTCTAATAAAGAA GATCCAGAACAGCTGAGATTAAAGCAGAAAGCCAAAGAGTTACAGCAATTGGAACTTGCACAGATACAG CATAGAGACGCTAATCTCACAGCTCTTGCAGCTATTGGACCAAGGAAGAAGAGACCACTAGAATCTGGA ATTGAGGGCTTAAAAGACAACCTTCTTGCTTCTGGGACATCCAGCCTGACAGCCACCAAACAGTTGCAT CGTCCAAGAATCACGAGAATCTGCCTCAGGGACTTGATATTTTGTATGGAACAGGAACGGGAGATGAAG TATTCTCGAGCTCTATACCTGGCCCTTCTGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_005640 |
| Insert Size | 2589 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005640.2 |
| RefSeq Size | 4339 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 6875 |
| UniProt ID | Q92750 |
| Protein Pathways | Basal transcription factors, Huntington's disease |
| MW | 91.1 kDa |
| Gene Summary | TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212361 | TAF4B (Myc-DDK-tagged)-Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B) |
CNY 6,312.00 |
|
| RC212361L3 | Lenti ORF clone of Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B), Myc-DDK-tagged |
CNY 11,020.00 |
|
| RC212361L4 | Lenti ORF clone of Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B), mGFP tagged |
CNY 11,020.00 |
|
| RG212361 | TAF4B (tGFP-tagged) - Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B) |
CNY 10,070.00 |
