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USP5 (NM_001098536) Human Untagged Clone
CAT＃: SC316428
USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ISOT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316428 representing NM_001098536.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGAC CGGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGT ATGAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTC TACTTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCC CGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAGAAGTTTGAA TTAGACGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGA CTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCC CGCAAGCAGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAG CAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAAC CTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAAC AACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCT GATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTG TCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGAC ATGAACCAGCGGATTGGTGAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGG CCTGGCTACACAGGCATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTC AGCATCCCTGACTTCCAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGAC CCTACCCAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCCAAG CCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGATGGCATTGCCCCT CGGATGTTCAAGGCCCTCATCGGCAAGGGCCACCCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAG GAGTTCTTCCTTCACCTTATCAACATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTG TTCCGCTTCTTGGTGGAGGAAAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTT GACTACATCATGCAGCTGCCTGTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAG GAGAAGAAGCGGCAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTC AGCTCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTGCAGGCC AAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTCCCTGACTACCTGGTCATCCAGATCAAGAAG TTCACCTTCGGCTTAGACTGGGTGCCCAAGAAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGAC ATCTCCCAGTTGAGGGGCACAGGGCTGCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTG GTCACTCCGGATGAGCCCAAAGGTAGCCTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCC CCTCACTTCTCCTCTCCGACATCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGA TTCCCTATGGACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAAC TGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCTGGCTCTAGTGGGCCG GGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTGACCACCATTGTCTCCATGGGCTTC TCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACGAACAATAGTTTAGAACGGGCTGTGGACTGGATC TTCAGTCACATTGACGACCTGGATGCTGAAGCTGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGAC TCCATCTCTGAGTCTGTGCCAGTGGGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCC TTCATTAGTCACATGGGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGA TGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGCTACATCTAC TTCTACCAGAGAGTGGCCAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001098536 |
| Insert Size | 2577 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001098536.1 |
| RefSeq Size | 3214 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 8078 |
| UniProt ID | P45974 |
| Protein Families | Druggable Genome, Protease |
| MW | 95.8 kDa |
| Gene Summary | Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224191 | USP5 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1 |
CNY 3,990.00 |
|
| RC224191L1 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224191L2 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC224191L3 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224191L4 | Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG224191 | USP5 (tGFP-tagged) - Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1 |
CNY 4,370.00 |
