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RASGRP2 (NM_001098670) Human Untagged Clone
CAT＃: SC316410
RASGRP2 (untagged)-Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 3
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CALDAG-GEFI; CDC25L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316410 representing NM_001098670.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGGCACCCTGGACCTGGACAAGGGCTGCACGGTGGAGGAGCTGCTCCGCGGGTGCATCGAAGCC TTCGATGACTCCGGGAAGGTGCGGGACCCGCAGCTGGTGCGCATGTTCCTCATGATGCACCCCTGGTAC ATCCCCTCCTCTCAGCTGGCGGCCAAGCTGCTCCACATCTACCAACAATCCCGGAAGGACAACTCCAAT TCCCTGCAGGTGAAAACGTGCCACCTGGTCAGGTACTGGATCTCCGCCTTCCCAGCGGAGTTTGACTTG AACCCGGAGTTGGCTGAGCAGATCAAGGAGCTGAAGGCTCTGCTAGACCAAGAAGGGAACCGACGGCAC AGCAGCCTAATCGACATAGACAGCGTCCCTACCTACAAGTGGAAGCGGCAGGTGACTCAGCGGAACCCT GTGGGACAGAAAAAGCGCAAGATGTCCCTGTTGTTTGACCACCTGGAGCCCATGGAGCTGGCGGAGCAT CTCACCTACTTGGAGTATCGCTCCTTCTGCAAGATCCTGTTTCAGGACTATCACAGTTTCGTGACTCAT GGCTGCACTGTGGACAACCCCGTCCTGGAGCGGTTCATCTCCCTCTTCAACAGCGTCTCACAGTGGGTG CAGCTCATGATCCTCAGCAAACCCACAGCCCCGCAGCGGGCCCTGGTCATCACACACTTTGTCCACGTG GCGGAGAAGCTGCTACAGCTGCAGAACTTCAACACGCTGATGGCAGTGGTCGGGGGCCTGAGCCACAGC TCCATCTCCCGCCTCAAGGAGACCCACAGCCACGTTAGCCCTGAGACCATCAAGCTCTGGGAGGGTCTC ACGGAACTAGTGACGGCGACAGGCAACTATGGCAACTACCGGCGTCGGCTGGCAGCCTGTGTGGGCTTC CGCTTCCCGATCCTGGGTGTGCACCTCAAGGACCTGGTGGCCCTGCAGCTGGCACTGCCTGACTGGCTG GACCCAGCCCGGACCCGGCTCAACGGGGCCAAGATGAAGCAGCTCTTTAGCATCCTGGAGGAGCTGGCC ATGGTGACCAGCCTGCGGCCACCAGTACAGGCCAACCCCGACCTGCTGAGCCTGCTCACGGTGTCTCTG GATCAGTATCAGACGGAGGATGAGCTGTACCAGCTGTCCCTGCAGCGGGAGCCGCGCTCCAAGTCCTCG CCAACCAGCCCCACGAGTTGCACCCCACCACCCCGGCCCCCGGTACTGGAGGAGTGGACCTCGGCTGCC AAACCCAAGCTGGATCAGGCCCTCGTGGTGGAGCACATCGAGAAGATGGTGGAGTCTGTGTTCCGGAAC TTTGACGTCGATGGGGATGGCCACATCTCACAGGAAGAATTCCAGATCATCCGTGGGAACTTCCCTTAC CTCAGCGCCTTTGGGGACCTCGACCAGAACCAGGATGGCTGCATCAGCAGGGAGGAGATGGTTTCCTAT TTCCTGCGCTCCAGCTCTGTGTTGGGGGGGCGCATGGGCTTCGTACACAACTTCCAGGAGAGCAACTCC TTGCGCCCCGTCGCCTGCCGCCACTGCAAAGCCCTGATCCTGGGCATCTACAAGCAGGGCCTCAAATGC CGAGCCTGTGGAGTGAACTGCCACAAGCAGTGCAAGGATCGCCTGTCAGTTGAGTGTCGGCGCAGGGCC CAGAGTGTGAGCCTGGAGGGGTCTGCACCCTCACCCTCACCCATGCACAGCCACCATCACCGCGCCTTC AGCTTCTCTCTGCCCCGCCCTGGCAGGCGAGGCTCCAGGCCTCCAGAGATCCGTGAGGAGGAGGTACAG ACGGTGGAGGATGGGGTGTTTGACATCCACTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001098670 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001098670.1 |
| RefSeq Size | 2244 bp |
| RefSeq ORF | 1830 bp |
| Locus ID | 10235 |
| UniProt ID | Q7LDG7 |
| Protein Pathways | Chemokine signaling pathway, MAPK signaling pathway |
| MW | 69.2 kDa |
| Gene Summary | The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 2. Variants 2, 3, and 4 all encode the same protein. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212672 | RASGRP2 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 3 |
CNY 4,536.00 |
|
| RC212672L3 | Lenti ORF clone of Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212672L4 | Lenti ORF clone of Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG212672 | RASGRP2 (tGFP-tagged) - Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 3 |
CNY 4,370.00 |
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