ketohexokinase (KHK) (NM_000221) Human Untagged Clone

CAT#: SC315989

KHK (untagged)-Human ketohexokinase (fructokinase) (KHK), transcript variant a



  "NM_000221" in other vectors (4)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
KHK (Ketohexokinase) mouse monoclonal antibody, clone OTI3D1 (formerly 3D1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000221 edited
TCGAGATCGCTTAGCCGCGCTTTAAAAAGGTTTGCATCAGCTGTGAGTCCATCTGACAAG
CGAGGAAACTAAGGCTGAGAAGTGGGAGGCGTTGCCATCTGCAGGCCCAGGCAACCTGCT
ACGGGAAGACCGGGGACCAAGACCTCTGGGTTGGCTTTCCTAGACCCGCTCGGGTCTTCG
GGTGTCGCGAGGAAGGGCCCTGCTCCTTTCGTTCCCTGCACCCCTGGCCGCTGCAGGTGG
CTCCCTGGAGGAGGAGCTCCCACGCGGAGGAGGAGCCAGGGCAGCTGGGAGCGGGGACAC
CATCCTCCTGGATAAGAGGCAGAGGCCGGGAGGAACCCCGTCAGCCGGGCGGGCAGGAAG
CTCTGGGAGTAGCCTCATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTAGTGGTGCTGGA
CGTCATCAGCCTGGTGGACAAGTACCCTAAGGAGGACTCGGAGATAAGGTGTTTGTCCCA
GAGATGGCAGCGCGGAGGCAACGCGTCCAACTCCTGCACCATTCTCTCCCTGCTCGGAGC
CCCCTGTGCCTTCATGGGCTCAATGGCTCCTGGCCATGTTGCTGATTTTGTCCTGGATGA
CCTCCGCCGTTATTCTGTGGACCTACGCTACACAGTCTTTCAGACCACAGGCTCCGTCCC
CATCGCCACGGTCATCATCAACGAGGCCAGTGGTAGCCGCACCATCCTATACTATGACAG
GAGCCTGCCAGATGTGTCTGCTACAGACTTTGAGAAGGTTGATCTGACCCAGTTCAAGTG
GATCCACATTGAGGGCCGGAACGCATCGGAGCAGGTGAAGATGCTGCAGCGGATAGACGC
ACACAACACCAGGCAGCCTCCAGAGCAGAAGATCCGGGTGTCCGTGGAGGTGGAGAAGCC
ACGAGAGGAGCTCTTCCAGCTGTTTGGCTACGGAGACGTGGTGTTTGTCAGCAAAGATGT
GGCCAAGCACTTGGGGTTCCAGTCAGCAGAGGAAGCCTTGAGGGGCTTGTATGGTCGTGT
GAGGAAAGGGGCTGTGCTTGTCTGTGCCTGGGCTGAGGAGGGCGCCGACGCCCTGGGCCC
TGATGGCAAATTGCTCCACTCGGATGCTTTCCCGCCACCCCGCGTGGTGGATACACTGGG
AGCTGGAGACACCTTCAATGCCTCCGTCATCTTCAGCCTCTCCCAGGGGAGGAGCGTGCA
GGAAGCACTGAGATTCGGGTGCCAGGTGGCCGGCAAGAAGTGTGGCCTGCAGGGCTTTGA
TGGCATCGTGTGAGAGCAGGTGCCGGCTCCTCACACACCATGGAGACTACCATTGCGGCT
GCATCGCCTTCTCCCCTCCATCCAGCCTGGCGTCCAGGTTGCCCTGTTCAGGGGACAGAT
GCAAGCTGTGGGGAGGACTCTGCCTGTGTCCTGTGTTCCCCACAGGGAGAGGCTCTGGGG
GGATGGCTGGGGGATGCAGAGCCTCAGAGCAAATAAATCTTCCTCAGAGCCAAAAAAAAA
AAAAAAAAAA
>OriGene 5' read for NM_000221 unedited
GAGACAGATGTATACGACTCCTATAGGGCGGCCGCGAACTCTCAGATCTGGTACCGATAT
CAAGCTTGTCGAGATTCGCTTAGCCGCGCTTTAAAAAGGTTTGCATCAGCTGTGAGTCCA
TCTGACAAGCGAGGAAACTAAGGCTGAGAAGTGGGAGGCGTTGCCATCTGCAGGCCCAGG
CAACCTGCTACGGGAAGACCGGGGACCAAGACCTCTGGGTTGGCTTTCCTAGACCCGCTC
GGGTCTTCGGGTGTCGCGAGGAAGGGCCCTGCTCCTTTCGTTCCCTGCACCCCTGGCCGC
TGCAGGTGGCTCCCTGGAGGAGGAGCTCCCACGCGGAGGAGGAGCCAGGGCAGCTGGGAG
CGGGGACACCATCCTCCTGGATAAGAGGCAGAGGCCGGGAGGAACCCCGTCAGCCGGGCG
GGCAGGAAGCTCTGGGAGTAGCCTCATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTAGT
GGTGCTGGACGTCATCAGCCTGGTGGACAAGTACCCTAAGGAGGACTCGGAGATAAGGTG
TTTGTCCCAGAGATGGCAGCGCGGAGGCAACGCGTCCAACTCCTGCACCATTCTCTCCCT
GCTCGGAGCCCCCTGTGCCTTCATGGGCTCAATGGCTCCTGGCCATGTTGCTGATTTTGT
CCTGGATGACCTCCGCCGTTATTCTGTGGACCTACGCTACACAGTCTTTCAGACCACAGG
CTCCGTCCCCATCGCCACGGTCATCATCAACGAGGCCAGTGGTAGCCGCACCATCCTATA
CTATGACAGGAGCCTGCCAGATGTGTCTGCTACAGACTTTGAGATGTTGATCTGACCCAG
TTCAAGTGGATCCACATTGAGGGCCGGACGCATCGGAGCAGTGAAGATGCTGCAGCGATA
GACGCACACACACCAGCAGCCTCCAGAGCAGAGATCCGGTGTCCGTGGGAGGTGGAGAAG
CCACGGAGAGGAGCTCTTTCAG
Restriction Sites Please inquire     
ACCN NM_000221
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a good match to NM_000221.2 except for one SNP.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000221.1, NP_000212.1
RefSeq Size 1899 bp
RefSeq ORF 897 bp
Locus ID 3795
UniProt ID P50053
Domains pfkB
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism, Metabolic pathways
Gene Summary This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant utilizes the alternate exon 3a and encodes isoform a, which exhibits lower enzymatic activity than isoform b.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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