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SART2 (DSE) (NM_001080976) Human Untagged Clone
CAT＃: SC315973
DSE (untagged)-Human dermatan sulfate epimerase (DSE), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DS-epi1; DSEP; DSEPI; EDSMC2; SART-2; SART2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315973 representing NM_001080976.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGACTCACACACGGGGGGCTCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTGTCAGCCTAC ATCACCGACGAGAACCCAGAAGTTATGATTCCCTTCACCAATGCCAACTACGACAGCCATCCCATGCTG TACTTCTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCTGCCAGCTCGCACGAGCACATTGCAGCC CGCCTCACGGAGGCTGTGCACACGATGCTGTCCAGCCCCTTGGAATACCTCCCTCCCTGGGATCCCAAG GACTACAGTGCCCGCTGGAATGAAATTTTTGGAAACAACTTGGGTGCCTTGGCAATGTTCTGTGTGCTG TATCCTGAGAACATTGAAGCCCGAGACATGGCCAAAGACTACATGGAGAGGATGGCAGCGCAGCCTAGT TGGTTGGTGAAAGATGCTCCTTGGGATGAGGTCCCGCTTGCTCACTCCCTGGTTGGTTTTGCCACTGCT TATGACTTCTTGTACAACTACCTGAGCAAGACACAACAGGAGAAGTTTCTTGAAGTGATTGCCAATGCC TCAGGGTATATGTATGAAACTTCATACAGGAGAGGATGGGGATTTCAATACCTGCACAATCATCAGCCC ACCAACTGTATGGCTTTGCTCACGGGAAGCCTAGTCCTGATGAATCAAGGATATCTTCAAGAAGCCTAC TTATGGACCAAACAAGTTCTGACCATCATGGAGAAATCTCTGGTCTTGCTCAGGGAGGTGACGGATGGC TCCCTCTATGAAGGAGTTGCGTATGGCAGCTACACCACTAGATCACTCTTCCAATACATGTTTCTCGTC CAGAGGCACTTCAACATCAACCACTTTGGCCATCCGTGGCTTAAACAACACTTTGCATTTATGTATAGA ACCATCCTGCCAGGGTTTCAAAGGACTGTGGCTATTGCGGACTCAAATTACAACTGGTTTTATGGTCCA GAAAGCCAATTAGTGTTCCTTGATAAATTTGTCATGCGTAATGGCAGTGGTAACTGGCTAGCTGACCAA ATCAGAAGGAACCGTGTGGTGGAAGGTCCAGGAACACCATCCAAAGGGCAGCGCTGGTGCACTCTGCAC ACAGAATTTCTCTGGTATGATGGCAGCTTGAAATCGGTTCCTCCTCCAGACTTTGGCACCCCTACACTG CATTATTTTGAAGACTGGGGTGTCGTGACTTATGGAAGTGCACTACCTGCAGAAATCAATAGATCTTTC CTTTCCTTCAAGTCTGGAAAACTGGGGGGACGTGCAATATATGACATTGTCCACAGAAACAAATACAAA GATTGGATCAAAGGATGGAGAAATTTTAATGCAGGGCATGAACATCCTGATCAAAACTCATTTACTTTT GCTCCCAATGGTGTGCCTTTCATTACTGAGGCTCTGTACGGGCCAAAGTACACCTTCTTCAACAATGTT TTGATGTTTTCCCCAGCTGTGTCAAAGAGCTGCTTTTCTCCCTGGGTGGGTCAGGTCACAGAAGACTGC TCATCAAAATGGTCTAAATACAAGCATGACCTGGCAGCTAGTTGTCAGGGGAGGGTGGTTGCAGCAGAG GAGAAAAATGGGGTGGTTTTCATCCGAGGAGAAGGTGTGGGAGCTTATAACCCCCAGCTCAACCTGAAG AATGTTCAGAGGAATCTCATCCTCCTACATCCACAGCTGCTTCTCCTTGTAGACCAAATACACCTGGGA GAGGAGAGTCCCTTGGAGACAGCAGCGAGCTTCTTCCATAATGTGGATGTTCCTTTTGAGGAGACTGTG GTAGATGGTGTCCATGGGGCTTTCATCAGGCAGAGAGATGGTCTCTATAAAATGTACTGGATGGACGAT ACTGGCTACAGCGAGAAAGCAACCTTTGCCTCAGTGACATATCCTCGGGGCTATCCCTACAACGGGACA AACTATGTGAATGTCACCATGCACCTCCGAAGTCCCATCACCAGGGCAGCTTACCTCTTCATAGGGCCA TCTATAGATGTTCAGAGCTTCACTGTCCACGGAGACTCTCAGCAACTGGATGTGTTCATAGCCACCAGC AAACATGCCTACGCCACATACCTGTGGACAGGTGAGGCCACAGGACAGTCTGCCTTTGCACAGGTCATT GCTGATCGTCACAAAATTCTGTTTGACCGGAATTCAGCCATCAAGAGCAGCATTGTCCCTGAGGTGAAG GACTATGCTGCTATTGTGGAACAGAACTTGCAGCATTTTAAACCAGTGTTTCAGCTGCTGGAGAAGCAG ATACTGTCCCGAGTCCGGAACACAGCTAGCTTTAGGAAGACTGCTGAACGCCTGCTGAGATTTTCAGAT AAGAGACAGACTGAGGAGGCCATTGACAGGATTTTTGCCATATCACAGCAACAGCAGCAGCAAAGCAAG TCAAAGAAAAACCGAAGGGCAGGCAAACGCTATAAATTTGTGGATGCTGTCCCTGATATTTTTGCACAG ATTGAAGTCAATGAGAAAAAGATTAGACAGAAAGCTCAGATTTTGGCACAGAAAGAACTACCCATAGAT GAAGATGAAGAAATGAAAGACCTTTTAGATTTTGCAGATGTAACATACGAGAAACATAAAAATGGGGGC TTGATTAAAGGCCGGTTTGGACAGGCACGGATGGTGACAACTACACACAGCAGGGCCCCATCACTGTCT GCTTCCTATACCAGGTTGTTCCTGATTCTGAACATTGCTATTTTCTTTGTCATGTTGGCAATGCAACTG ACTTATTTCCAGAGGGCCCAGAGCCTACATGGCCAAAGATGTCTTTATGCAGTTCTTCTCATAGATAGC TGTATTTTATTATGGTTGTACTCTTCTTGTTCCCAATCACAGTGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001080976 |
| Insert Size | 2877 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080976.2 |
| RefSeq Size | 4092 bp |
| RefSeq ORF | 2877 bp |
| Locus ID | 29940 |
| UniProt ID | Q9UL01 |
| Protein Families | Transmembrane |
| Protein Pathways | Chondroitin sulfate biosynthesis |
| MW | 109.8 kDa |
| Gene Summary | The protein encoded by this gene is a tumor-rejection antigen. It is localized to the endoplasmic reticulum and functions to convert D-glucuronic acid to L-iduronic acid during the biosynthesis of dermatan sulfate. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. Mutations in this gene cause inmusculocontractural Ehlers-Danlos syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9, and a paralogous gene exists on chromosome 18. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 all encode the same isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218893 | DSE (Myc-DDK-tagged)-Human dermatan sulfate epimerase (DSE), transcript variant 2 |
CNY 7,016.00 |
|
| RC218893L3 | Lenti ORF clone of Human dermatan sulfate epimerase (DSE), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218893L4 | Lenti ORF clone of Human dermatan sulfate epimerase (DSE), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG218893 | DSE (tGFP-tagged) - Human dermatan sulfate epimerase (DSE), transcript variant 2 |
CNY 8,616.00 |
