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DGKG (NM_001080744) Human Untagged Clone
CAT＃: SC315970
DGKG (untagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAGK3; DGK-GAMMA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315970 representing NM_001080744.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTGAAGAACGGTGGGTCTCCCTCACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATAT TCCTCCAAGAAGATAAAAGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCAAACAATATGACCCA CATGAGCCGATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTGGAGGTGGACCTTCCCCAG CCACTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCTGACCACCCGACG GAGGGAGCCAGCAACAGTGAGGCCAACAGCGCAGATACTAATATACAGAATGCAGATAATGCCACCAAA GCAGACGAGGCCTGTGCCCCTGATACTGAATCAAATATGGCTGAGAAGCAAGCACCAGCTGAAGACCAA GTGGCTGCGACCCCCCTGGAACCCCCCGTCCCTCGGTCTTCAAGCTCGGAATCCCCAGTGGTATACCTG AAGGATGTTGTGTGCTACCTGTCCCTGCTGGAGACGGGGAGGCCTCAGGATAAGCTGGAGTTCATGTTT CGCCTCTATGATTCAGATGAGAACGGTCTCCTGGACCAAGCGGAGATGGATTGCATTGTCAACCAAATG CTGCATATTGCCCAGTACCTGGAGTGGGATCCCACAGAGCTGAGGCCTATATTGAAGGAGATGCTGCAA GGGATGGACTACGACCGGGACGGCTTTGTGTCTCTACAGGAATGGGTCCATGGAGGGATGACCACCATC CCATTGCTGGTCCTCCTGGGGATGGATGACTCTGGCTCCAAGGGGGATGGGCGGCACGCCTGGACCATG AAGCACTTCAAGAAACCAACCTACTGCAACTTCTGCCATATCATGCTCATGGGCGTCCGCAAGCAAGGC CTGTGCTGCACTTACTGTAAATACACTGTCCACGAACGCTGTGTGTCCAGAAACATTCCTGGTTGTGTC AAAACGTACTCAAAAGCCAAAAGGAGTGGTGAGGTGATGCAGCACGCATGGGTGGAAGGGAACTCCTCC GTCAAGTGTGACCGGTGCCACAAAAGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGCGTGTGG TGCCGGATGACGTTTCACCGCAAATGTGAATTATCAACGTTGTGTGACGGTGGGGAACTCAGAGACCAC ATCTTACTGCCCACCTCCATATGCCCCATCACCCGGGACAGGCCAGGTGAGAAGTCTGATGGCTGCGTG TCCGCCAAGGGCGAACTTGTCATGCAGTATAAGATCATCCCCACCCCGGGTACCCACCCCCTGCTGGTC TTGGTGAACCCCAAGAGTGGAGGGAGACAAGGAGAAAGGTTGAACTTTTTCCGTGATACTCCAGACTTC CGTGTTTTGGCCTGTGGTGGAGATGGGACAGTTGGCTGGATTTTGGATTGCATTGATAAGGCCAACTTT GCAAAGCATCCACCAGTGGCTGTCCTGCCTCTTGGAACAGGAAATGACCTTGCCCGTTGTCTCCGCTGG GGAGGAGGTTATGAAGGGGGCAGCTTGACAAAAATCCTGAAAGACATTGAGCAGAGCCCCTTGGTGATG CTGGACCGCTGGCATCTGGAAGTCATCCCCAGAGAGGAAGTGGAAAACGGGGACCAGGTCCCATACAGC ATCATGAACAACTATTTCTCCATTGGTGTGGACGCTTCCATTGCACACAGATTCCATGTGATGAGAGAG AAACATCCTGAAAAATTCAACAGCAGGATGAAGAACAAGCTGTGGTACTTTGAATTTGGCACCTCGGAG ACTTTTGCAGCGACCTGCAAGAAACTCCACGACCACATTGAGTTGGAGTGTGATGGGGTTGGGGTGGAC CTGAGCAACATCTTCCTGGAAGGCATTGCCATTCTCAACATTCCCAGCATGTACGGAGGCACCAATCTC TGGGGAGAAAACAAGAAGAACCGGGCTGTGATCCGGGAAAGCAGGAAGGGTGTCACTGACCCCAAAGAA CTGAAATTCTGCGTTCAAGACCTCAGTGACCAGCTCCTTGAAGTGGTGGGGCTAGAAGGAGCCATGGAG ATGGGGCAGATCTACACCGGCCTGAAGAGTGCAGGCAGGAGGCTGGCCCAGTGCGCCTCTGTCACCATC AGGACAAACAAGCTGCTGCCAATGCAAGTGGATGGAGAACCCTGGATGCAGCCATGTTGCACGATTAAA ATTACTCACAAGAACCAAGCGCCCATGATGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGA AGGAAGAGCCGTTCAAAAGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001080744 |
| Insert Size | 2301 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080744.1 |
| RefSeq Size | 5743 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 1608 |
| UniProt ID | P49619 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 86.3 kDa |
| Gene Summary | This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224710 | DGKG (Myc-DDK-tagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 2 |
CNY 5,608.00 |
|
| RC224710L3 | Lenti ORF clone of Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224710L4 | Lenti ORF clone of Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 2, mGFP tagged |
CNY 8,008.00 |
|
| RG224710 | DGKG (tGFP-tagged) - Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 2 |
CNY 4,370.00 |
