United States
Germany
Japan
United Kingdom
China
POMT1 (NM_001077366) Human Untagged Clone
CAT＃: SC315608
POMT1 (untagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LGMD2K; LGMDR11; MDDGA1; MDDGB1; MDDGC1; RT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315608 representing NM_001077366.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAACAAATCTTCTTCTTGGATGACAGTGGGCCGCCATTTGGCCACATGGTGCTGGCCTTGGGAGGT TATTTAGGAGGATTCGATGGCAATTTTTTGTGGAACAGAATTGGAGCAGAATACAGTAGCAACGTGCCT GTGTGGTCCCTGCGCCTGCTGCCAGCACTCGCGGGGGCCTTGTCGGTCCCCATGGCCTACCAGATAGTG TTGGAGCTCCACTTTTCTCATTGTGCCGCCATGGGAGCTGCTCTGTTGATGCTTATCGAGAATGCTCTC ATCACTCAGTCAAGGCTAATGCTTTTGGAATCAGTGTTAATATTTTTCAATCTATTGGCCGTGTTGTCC TACCTGAAGTTCTTCAACTGCCAAAAGCACAGCCCTTTTTCTCTGAGCTGGTGGTTCTGGCTAACACTG ACAGGGGTCGCTTGTTCCTGTGCAGTGGGCATCAAGTACATGGGTGTGTTCACGTACGTGCTCGTGCTG GGTGTTGCAGCTGTCCATGCCTGGCACCTGCTTGGAGACCAGACTTTGTCCAATGTCTGTGTGTTCTGT CACTTGCTCGCCCGAGCAGTGGCTTTGCTGGTCATCCCGGTCGTCCTGTACTTACTGTTCTTCTACGTC CACTTGATTCTAGTCTTCCGCTCTGGGCCCCACGACCAAATCATGTCCAGTGCCTTCCAGGCCAGCTTA GAGGGAGGACTAGCTCGGATCACCCAGGGTCAGCCACTGGAGGTGGCCTTTGGGTCCCAGGTCACTCTG AGGAACGTCTTTGGGAAACCTGTGCCCTGCTGGCTTCATTCCCACCAGGACACCTACCCCATGATATAT GAGAACGGCCGAGGCAGCTCCCACCAGCAACAGGTGACCTGTTACCCCTTCAAAGACGTCAATAACTGG TGGATTGTAAAGGATCCCAGGAGGCACCAGCTGGTGGTGAGCAGCCCTCCGAGACCTGTGAGGCACGGG GACATGGTGCAGCTGGTCCACGGCATGACCACCCGCTCCCTGAACACGCATGATGTTGCAGCCCCCCTG AGCCCCCATTCACAGGAGGTCTCCTGCTACATTGACTATAACATCTCCATGCCCGCCCAGAACCTCTGG AGACTGGAAATTGTGAACAGAGGATCTGACACAGACGTCTGGAAGACCATCCTCTCAGAGGTCCGCTTT GTGCACGTGAACACTTCCGCTGTCTTAAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTATCGGCAA CTGGAGATCGTCGGGGAGAAGCTGTCCCGGGGCTACCACGGGAGCACGGTGTGGAACGTGGAGGAGCAC CGATACGGCGCGAGCCAGGAGCAGAGGGAGCGGGAACGGGAGCTGCACTCACCTGCGCAGGTGGACGTC AGCAGGAACCTCAGCTTCATGGCGAGATTCTCGGAGCTGCAGTGGAGGATGCTGGCGCTGAGAAGTGAT GACTCGGAACACAAGTACAGCTCCAGCCCACTGGAGTGGGTCACCCTGGACACCAATATTGCCTACTGG CTGCACCCCAGGACCAGCGCTCAGATCCACCTACTTGGAAACATAGTGATCTGGGTTTCGGGCAGCCTC GCTCTGGCCATCTACGCCCTGCTGTCCTTGTGGTACCTGCTCCGACGGCGAAGAAATGTCCATGACCTC CCTCAGGATGCCTGGCTGCGCTGGGTGCTGGCTGGGGCGCTGTGTGCCGGTGGCTGGGCAGTGAACTAC CTCCCGTTCTTCCTGATGGAGAAGACACTCTTCCTCTACCACTACCTGCCCGCACTCACCTTCCAAATC CTTCTGCTCCCTGTGGTCCTGCAGCACATCAGCGACCACCTGTGCAGGTCCCAGCTCCAGAGGAGCATC TTCAGCGCCCTGGTGGTGGCCTGGTACTCCTCCGCGTGCCACGTGTCCAACACGCTGCGCCCACTCACC TACGGGGACAAGTCACTCTCGCCACATGAACTCAAGGCCCTTCGCTGGAAAGACAGCTGGGACATCTTG ATCCGAAAACACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001077366 |
| Insert Size | 2016 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001077366.1 |
| RefSeq Size | 2928 bp |
| RefSeq ORF | 2016 bp |
| Locus ID | 10585 |
| UniProt ID | Q9Y6A1 |
| Protein Families | Transmembrane |
| Protein Pathways | O-Mannosyl glycan biosynthesis |
| MW | 76.3 kDa |
| Gene Summary | The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008] Transcript Variant: This variant (3) uses an alternate in-frame splice site and lacks the exon containing the translation start site compared to variant 1. The resulting isoform (c) is shorter at the N-terminus and lacks an internal segment compared to isoform a. Variants 3 and 7 both encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209439 | POMT1 (Myc-DDK-tagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3 |
CNY 5,320.00 |
|
| RC209439L3 | Lenti ORF clone of Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209439L4 | Lenti ORF clone of Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3, mGFP tagged |
CNY 7,700.00 |
|
| RG209439 | POMT1 (tGFP-tagged) - Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3 |
CNY 6,370.00 |
