alpha 1 Spectrin (SPTA1) (NM_003126) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EL2; HPP; HS3; SPH3; SPTA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315387 representing NM_003126. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCAATTTCCAAAGGAAACCGTTGTGGAGAGCAGTGGGCCAAAGGTTTTGGAAACAGCAGAAGAG ATCCAGGAGAGGCGTCAGGAAGTGTTGACTCGGTATCAAAGTTTCAAGGAGCGGGTCGCTGAGAGGGGT CAGAAGCTTGAGGATTCCTATCACTTACAAGTTTTCAAGCGAGATGCAGATGATCTGGGGAAGTGGATC ATGGAGAAAGTCAATATCTTAACCGATAAGAGCTATGAAGACCCAACTAATATACAGGGGAAATATCAG AAGCATCAATCCCTTGAAGCAGAGGTGCAAACAAAATCAAGACTCATGTCTGAACTGGAAAAAACAAGG GAAGAACGATTTACCATGGGTCATTCTGCCCACGAAGAAACGAAGGCCCATATAGAGGAGCTACGCCAC CTGTGGGACCTGCTGTTAGAGCTGACCCTGGAGAAGGGTGACCAGTTGCTGCGGGCCCTGAAGTTCCAG CAGTATGTACAGGAGTGTGCTGACATCTTAGAGTGGATTGGAGACAAGGAGGCTATAGCGACATCAGTG GAGCTAGGTGAAGACTGGGAGCGCACCGAAGTTCTGCATAAGAAATTTGAAGACTTCCAAGTGGAGCTG GTAGCTAAAGAAGGGAGAGTTGTTGAAGTGAACCAATATGCCAATGAGTGTGCCGAGGAAAACCATCCT GACCTACCCTTAATTCAGTCTAAGCAAAATGAGGTGAATGCTGCCTGGGAGCGCCTTCGTGGTTTGGCT CTCCAGAGACAGAAAGCTCTGTCCAATGCTGCAAACTTACAACGATTCAAAAGGGATGTGACTGAAGCC ATCCAGTGGATCAAGGAGAAGGAACCTGTACTCACCTCTGAGGACTATGGCAAAGACCTTGTTGCCTCT GAAGGACTGTTTCACAGTCACAAGGGACTTGAGAGAAATCTTGCTGTCATGAGTGACAAGGTGAAGGAG TTATGTGCTAAAGCAGAGAAGCTGACACTTTCCCATCCTTCAGATGCACCTCAGATCCAGGAGATGAAA GAAGATCTGGTCTCCAGCTGGGAGCATATTCGTGCCCTGGCCACCAGCAGATATGAAAAACTGCAGGCT ACTTATTGGTACCATCGATTTTCATCTGACTTTGATGAACTCTCAGGCTGGATGAACGAGAAGACTGCT GCGATCAATGCTGATGAGCTGCCAACAGATGTGGCTGGTGGAGAAGTTCTGCTGGACAGGCATCAGCAG CATAAGCATGAGATTGACTCTTACGATGACCGATTTCAATCTGCTGATGAGACTGGTCAAGACCTCGTG AATGCCAATCATGAAGCCTCTGATGAAGTTCGGGAAAAGATGGAAATACTTGACAACAACTGGACTGCC CTGCTGGAACTGTGGGACGAGCGTCATCGTCAGTATGAGCAGTGCTTGGACTTTCATCTCTTCTACAGA GACAGTGAGCAAGTGGACAGTTGGATGAGTAGACAAGAGGCCTTCCTGGAAAACGAGGATCTGGGAAAC TCACTGGGCAGTGCAGAAGCCCTTCTTCAGAAGCATGAAGACTTTGAGGAAGCCTTTACTGCCCAGGAA GAGAAGATCATAACTGTAGACAAGACTGCAACCAAATTGATTGGTGATGACCATTATGATTCAGAGAAC ATCAAGGCTATCCGTGACGGGCTGTTAGCCCGGCGGGATGCCCTACGTGAAAAGGCTGCCACTAGACGT AGATTGCTGAAGGAGTCATTGCTTCTGCAAAAACTGTATGAGGACTCAGATGACCTAAAGAACTGGATC AACAAGAAGAAAAAGTTGGCAGATGATGAAGATTACAAGGACATACAGAACTTGAAGAGCAGGGTTCAA AAGCAGCAAGTCTTTGAAAAGGAGTTGGCAGTTAATAAGACCCAGCTGGAAAACATACAGAAAACTGGC CAAGAGATGATTGAGGGTGGTCACTATGCCTCTGACAATGTGACCACTCGTCTGAGTGAAGTTGCCAGC CTCTGGGAGGAGTTGCTGGAGGCTACAAAACAGAAAGGGACCCAGTTGCATGAGGCCAACCAGCAGCTG CAATTTGAAAATAATGCAGAAGATTTGCAGCGCTGGCTGGAGGATGTTGAGTGGCAAGTCACCTCTGAG GATTATGGGAAAGGCCTGGCCGAGGTACAGAATCGACTCAGGAAACACGGCCTCCTGGAGTCGGCTGTG GCTGCTCGTCAGGATCAGGTGGATATCCTTACAGACCTGGCTGCATATTTTGAAGAAATAGGCCATCCT GATTCTAAGGATATAAGGGCAAGGCAAGAGTCCTTGGTATGCCGATTTGAAGCTCTGAAAGAGCCACTG GCCACCCGAAAGAAGAAGCTCTTAGACCTTCTCCATCTGCAGCTGATTTGTAGAGACACAGAGGATGAG GAGGCCTGGATCCAAGAGACTGAACCCTCAGCTACTTCCACCTACCTTGGAAAGGACCTGATTGCTTCC AAAAAGCTTCTGAATAGGCATAGAGTCATCCTGGAGAACATTGCCAGCCATGAACCACGCATTCAAGAG ATAACAGAAAGGGGAAACAAAATGGTAGAGGAAGGACACTTTGCTGCAGAAGATGTGGCCTCTAGGGTC AAGAGTTTGAACCAGAATATGGAGTCTCTCCGTGCTCGAGCTGCTAGGCGACAAAATGATCTTGAAGCC AATGTCCAGTTCCAGCAGTACCTGGCTGACCTGCATGAAGCAGAAACATGGATCAGAGAGAAGGAACCT ATTGTAGATAATACTAACTATGGTGCTGATGAAGAAGCAGCTGGGGCTCTTCTAAAGAAGCATGAGGCC TTTCTATTAGATCTCAATTCATTTGGAGACAGTATGAAAGCTCTGCGGAATCAGGCAAACGCCTGCCAG CAACAACAGGCTGCACCAGTGGAGGGAGTTGCTGGAGAACAAAGGGTCATGGCTTTATATGACTTCCAG GCCCGCAGCCCCCGAGAAGTCACCATGAAGAAAGGTGATGTCTTAACGCTGCTCAGTTCCATCAATAAG GACTGGTGGAAGGTGGAAGCTGCTGATCATCAGGGCATTGTCCCAGCTGTCTATGTCAGAAGACTGGCC CACGATGAGTTCCCGATGCTCCCACAGCGGCGACGAGAAGAGCCAGGAAACATCACCCAGCGCCAGGAG CAGATTGAGAACCAATACCGCTCCCTCTTGGATCGGGCAGAAGAACGCAGACGTCGTCTATTGCAACGT TATAATGAATTTTTATTGGCCTATGAGGCAGGAGACATGCTGGAATGGATTCAAGAGAAAAAGGCAGAA AACACTGGAGTGGAACTAGATGATGTTTGGGAGCTGCAGAAAAAGTTTGATGAGTTCCAAAAGGATTTG AATACCAATGAGCCTCGGCTAAGGGATATCAACAAGGTAGCTGATGATCTACTATTTGAAGGACTTCTA ACACCAGAAGGAGCTCAAATCCGGCAGGAATTGAATTCCCGCTGGGGTTCTTTGCAGAGGCTTGCAGAT GAACAGCGGCAGCTGCTGGGCAGTGCCCATGCTGTTGAAGTGTTTCACAGAGAAGCAGATGACACGAAG GAGCAGATTGAGAAGAAATGCCAGGCCCTCAGTGCTGCAGACCCTGGCTCAGATCTGTTCAGTGTTCAG GCTCTTCAGCGACGGCATGAGGGCTTTGAAAGGGACCTCGTACCCCTGGGAGATAAGGTGACCATACTG GGGGAGACAGCAGAGCGGCTCAGTGAGTCCCATCCAGATGCCACTGAGGACCTGCAGAGACAGAAAATG GAGCTGAATGAGGCCTGGGAAGACCTGCAGGGGCGTACAAAGGATCGTAAGGAGAGCCTAAATGAGGCC CAGAAATTCTACCTGTTCCTCAGCAAGGCCAGGGATCTGCAGAACTGGATCAGTAGCATTGGTGGCATG GTATCATCACAGGAGCTGGCCGAAGACTTAACTGGCATAGAGATCTTGCTGGAGAGACATCAGGAGCAC CGTGCTGACATGGAGGCAGAGGCTCCCACCTTCCAGGCCTTAGAGGACTTCAGTGCAGAACTTATCGAC AGTGGGCACCATGCTAGCCCTGAAATTGAAAAAAAGCTTCAAGCTGTCAAGCTAGAGAGAGATGATTTG GAGAAGGCTTGGGAAAAACGCAAGAAGATCCTAGACCAGTGCCTGGAGTTGCAGATGTTCCAGGGGAAC TGTGATCAAGTTGAGAGCTGGATGGTGGCACGTGAGAATTCCCTGAGGTCAGATGACAAAAGTTCCTTA GACAGTCTGGAGGCTTTGATGAAGAAACGGGACGATTTGGACAAAGCAATCACTGCCCAGGAAGGGAAG ATCACTGACCTAGAACATTTTGCTGAGAGCCTCATTGCTGATGAACACTATGCCAAAGAAGAGATTGCT ACGCGGCTCCAACGTGTACTAGACAGGTGGAAGGCTCTCAAAGCACAACTGATTGATGAGCGGACAAAG CTTGGAGACTATGCCAACCTAAAACAATTCTACCGAGACCTTGAGGAGCTGGAAGAATGGATCAGTGAG ATGCTGCCCACAGCCTGTGATGAATCCTACAAAGACGCCACTAACATTCAGAGGAAATACCTGAAACAC CAGACCTTTGCACATGAAGTCGATGGCCGATCTGAGCAGGTGCATGGCGTCATCAACCTGGGGAACTCC CTGATTGAGTGTAGCGCTTGTGATGGCAATGAAGAGGCCATGAAGGAGCAACTGGAACAGCTGAAGGAA CATTGGGATCATCTGCTTGAGAGAACAAATGACAAAGGGAAGAAGCTCAATGAGGCCAGTCGTCAACAG AGGTTCAACACAAGCATCCGGGACTTTGAGTTCTGGCTCTCAGAGGCAGAGACATTGCTGGCCATGAAA GATCAGGCCAGGGACTTGGCTTCAGCAGGAAACCTACTCAAGAAGCATCAGCTATTGGAGAGAGAGATG TTGGCTCGAGAGGATGCACTCAAGGACCTGAATACATTGGCTGAAGATTTGCTCTCCAGCGGGACTTTC AACGTTGATCAGATTGTGAAGAAAAAAGATAATGTCAACAAGCGTTTCCTGAATGTCCAAGAATTGGCA GCTGCACACCACGAAAAATTGAAAGAGGCCTATGCCTTGTTCCAGTTCTTCCAGGATCTAGATGATGAG GAATCCTGGATAGAGGAGAAGTTGATACGAGTGAGCTCCCAGGACTATGGGAGAGATCTTCAGGGGGTT CAGAACTTGCTGAAGAAGCACAAACGCCTAGAGGGGGAGCTGGTGGCCCATGAGCCTGCCATCCAGAAT GTGCTGGATATGGCAGAGAAGCTGAAAGACAAGGCTGCTGTGGGGCAAGAGGAGATCCAGTTGCGGCTG GCTCAGTTTGTTGAACACTGGGAGAAGCTCAAAGAGTTGGCCAAGGCCCGAGGACTTAAGTTGGAAGAA TCCCTAGAATACTTGCAATTCATGCAGAATGCTGAGGAAGAGGAAGCTTGGATCAATGAAAAGAATGCT TTGGCTGTCCGAGGAGATTGTGGAGATACATTAGCTGCTACTCAGAGCTTGCTAATGAAGCATGAAGCT TTGGAAAATGACTTTGCTGTCCATGAGACCCGAGTACAAAATGTGTGTGCACAAGGAGAAGACATCCTA AATAAGGTGTTGCAGGAGGAAAGTCAGAACAAAGAGATTTCTTCCAAGATAGAGGCTCTGAATGAAAAG ACCCCTTCTCTGGCTAAGGCAATAGCTGCTTGGAAGTTGCAATTGGAAGACGATTATGCCTTTCAGGAA TTCAACTGGAAGGCTGATGTGGTAGAGGCTTGGATAGCTGATAAGGAAACAAGCCTAAAGACCAATGGC AATGGTGCAGACCTTGGTGACTTCCTCACTCTTCTGGCAAAACAGGACACTCTGGATGCCAGTCTGCAG AGTTTCCAGCAAGAGAGACTTCCCGAGATCACTGACCTGAAGGACAAACTGATTTCTGCTCAACACAAC CAGTCTAAAGCCATTGAAGAGCGTTATGCCGCTCTGCTGAAGCGCTGGGAACAGTTGCTGGAAGCCTCG GCAGTCCACAGACAGAAATTGCTGGAGAAACAGCTGCCTCTACAGAAGGCTGAGGACCTGTTCGTGGAA TTTGCACATAAGGCTTCAGCTTTGAACAACTGGTGTGAAAAGATGGAAGAAAACTTGTCAGAGCCTGTG CACTGTGTCTCCCTGAATGAAATTCGGCAGCTGCAGAAAGACCATGAGGACTTCTTGGCCTCCCTGGCT AGGGCTCAAGCAGACTTTAAATGTTTGCTGGAGCTAGACCAGCAGATTAAGGCCTTAGGTGTGCCTTCC AGCCCTTATACCTGGTTAACAGTGGAGGTGCTGGAAAGGACCTGGAAGCACCTATCTGACATCATTGAG GAACGGGAGCAGGAGCTGCAAAAGGAAGAGGCAAGACAGGTCAAGAACTTTGAGATGTGTCAGGAGTTT GAACAGAATGCCAGTACCTTCCTTCAATGGATCCTGGAAACCAGGGCTTACTTTCTGGATGGATCATTG CTCAAAGAAACAGGAACTCTGGAATCTCAGCTGGAAGCAAATAAAAGAAAACAGAAGGAGATCCAGGCG ATGAAGCGTCAACTAACCAAGATTGTGGACCTGGGGGACAACTTGGAAGACGCTCTGATCCTTGATATC AAATACAGCACCATTGGATTGGCTCAGCAGTGGGACCAGCTCTACCAGCTTGGGTTGCGGATGCAACAC AACCTGGAGCAACAGATCCAGGCCAAGGACATCAAAGGTGTGAGTGAAGAGACTCTAAAGGAATTTAGC ACAATCTATAAACACTTTGATGAGAATTTGACAGGGCGCCTGACTCACAAAGAGTTCCGGTCCTGCCTG AGAGGACTCAATTACTACTTGCCCATGGTGGAGGAGGATGAACATGAGCCCAAGTTTGAGAAGTTCCTG GATGCTGTGGATCCAGGGAGGAAGGGCTATGTCTCACTGGAGGACTATACTGCTTTCCTGATTGACAAG GAGTCAGAAAACATCAAGTCCAGTGATGAAATAGAGAATGCCTTCCAAGCCCTGGCAGAGGGCAAGTCA TATATTACCAAAGAAGACATGAAGCAGGCCCTTACCCCAGAGCAAGTGTCATTCTGTGCCACACATATG CAGCAATATATGGACCCACGGGGTCGAAGCCATCTCTCTGGCTATGACTACGTTGGCTTCACCAATTCC TACTTTGGCAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_003126
Insert Size	7260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003126.2
RefSeq Size	8017 bp
RefSeq ORF	7260 bp
Locus ID	6708
UniProt ID	P02549
Domains	SH3, spectrin, EFh
MW	280 kDa
Gene Summary	This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017]

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