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DOT1L (NM_032482) Human Untagged Clone
CAT＃: SC315370
DOT1L (untagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
CNY 29,550.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DOT1; KMT4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_032482 edited
GGCACGAGGCGGGCCTGTGACTACAAAGAGGGAGTCGGGGGCCGGGCCGGACCGGAGCGC GGCGGCGGCGGCGGCGGCGGCCGAGGCCGAGGCCAGGCCCCCTCCCCTCAGCCTCCCGCC CCTCCCTCCCGCCCGCCCTCCTCCGCCCACCGGCGGCCCCGCCCCTCCCCCAACCGCCCG CCTAGCATGGTGCGGCGGCCGCGCGCGCGGACATGGGGGAGAAGCTGGAGCTGAGACTGA AGTCGCCCGTGGGGGCTGAGCCCGCCGTCTACCCGTGGCCGCTGCCGGTCTACGCTAAAC ATCACGATGCTGCTCATGAAATCATCGAGACCATCCGATGGGTCTGTGAAGAAATCCCGG ATCTCAAGCTCGCTATGGAGAATTACGTTTTAATTGACTATGACACCAAAAGCTTCGAGA GCATGCAGAGGCTCTGCGACAAGTACAACCGTGCCATCGACAGCATCCACCAGCTGTGGA AGGGCACCACGCAGCCCATGAAGCTGAACACGCGGCCGTCCACTGGACTCCTGCGCCATA TCCTGCAGCAGGTCTACAACCACTCGGTGACCGACCCCGAGAAGCTCAACAACTACGAGC CCTTCTCCCCCGAGGTGTACGGGGAGACCTCCTTCGACCTGGTGGCCCAGATGATTGATG AGATCAAGATGACCGACGACGACCTGTTTGTGGACTTGGGGAGCGGTGTGGGCCAGGTCG TGCTCCAGGTTGCTGCTGCCACCAACTGCAAACATCACTATGGCGTCGAGAAAGCAGACA TCCCGGCCAAGTATGCGGAGACCATGGACCGCGAGTTCAGGAAGTGGATGAAATGGTATG AAAAAAAGCATGCAGAATACACATTGGAGAGAGGCGATTTCCTCTCAGAAGAGTGGAGGG AGCGAATCGCCAACACGAGTGTTATATTTGTGAATAATTTTGCCTTTGGTCCTGAGGTGG ATCACCAGCTGAAGGAGCGGTTTGCAAACATGAAGGAAGGTGGCAGAATCGTGTCCTCGA AACCCTTTGCACCTCTGAACTTCAGAATAAACAGTAGAAACTTGAGTGACATCGGCACCA TCATGCGCGTGGTGGAGCTCTCGCCCCTGAAGGGCTCGGTGTCGTGGACGGGGAAGCCAG TCTCCTACTACCTGCACACTATCGACCGCACCATACTTGAAAACTATTTTTCTAGTCTGA AAAACCCAAAACTCAGGGAGGAACAGGAGGCAGCCCGGCGCCGCCAGCAGCGCGAGAGCA AGAGCAACGCGGCCACGCCCACTAAGGGCCCAGAGGGCAAGGTGGCCGGCCCCGCCGACG CCCCCATGGACTCTGGTGCTGAGGAAGAGAAGGCGGGAGCAGCCACCGTGAAGAAGCCGT CTCCCTCCAAAGCCCGCAAGAAGAAGCTAAACAAGAAGGGGAGGAAGATGGCTGGCCGCA AGCGCGGGCGCCCCAAGAAGATGAACACTGCGAACCCCGAGCGGAAGCCCAAGAAGAACC AAACTGCACTGGATGCCCTGCACGCTCAGACCGTGTCTCAGACGGCGGCCTCCTCACCCC AGGATGCCTACAGATCCCCTCACAGCCCGTTCTACCAGCTACTTCCGAGCGTGCAGCGGC ACTCCCCCAACCCGCTGCTGGTGGCGCCCACCCCGCCCGCGCTGCAGAAGCTTCTAGAGT CCTTCAAGATCCAGTACCTGCAGTTCCTGGCATACACAAAGACCCCCCAGTACAAGGCCA GCCTGCAGGAGCTGCTGGGCCAGGAGAAGGAGAAGAACGCCCAGCTCCTGGGTGCGGCTC AGCAGCTCCTCAGCCACTGCCAGGCCCAGAAGGAGGAGATCAGGAGGCTGTTTCAGCAAA AATTGGATGAGCTGGGTGTGAAGGCGCTGACCTACAACGACCTGATTCAAGCGCAGAAGG AGATCTCCGCCCATAACCAGCAGCTGCGGGAGCAGTCGGAGCAGCTGGAGCAGGACAACC GCGCGCTCCGCGGCCAGAGCTTGCAGCTGCTCAAGGCTCGCTGCGAGGAGCTGCAGCTGG ACTGGGCCACGCTGTCGCTGGAGAAGCTGTTGAAGGAGAAGCAGGCCCTGAAGAGCCAGA TCTCGGAGAAGCAGAGGCACTGCCTGGAGCTGCAGATCAGCATTGTGGAGCTAGAGAAGA GCCAGCGGCAGCAGGAGCTCCTGCAGCTCAAGTCCTGTGTGCCGCCTGACGACGCCCTGT CCCTGCACCTGCGTGGGAAGGGCGCCCTGGGCCGCGAGCTGGAGCCTGACGCCAGCCGGC TGCACCTGGAGCTGGACTGCACCAAGTTCTCGCTGCCTCACTTGAGCAGCATGAGCCCGG AGCTCTCCATGAACGGCCAGGCTGCTGGCTATGAGCTCTGCGGTGTGCTGAGCCGGCCTT CGTCGAAGCAGAACACGCCCCAGTACCTGGCCTCACCCCTGGACCAGGAGGTGGTGCCCT GTACCCCTAGCCACGTCGGCCGGCCGCGCCTGGAGAAGCTGTCTGGCCTAGCCGCACCCG ACTACACTAGGCTGTCCCCGGCCAAGATTGTGCTGAGGCGGCACCTGAGCCAGGACCACA CGGTGCCCGGCAGGCCGGCTGCCAGTGAGCTGCATTCGAGAGCTGAGCACACCAAGGAGA ACGGCCTTCCCTACCAGAGCCCCAGCGTGCCTGGCAGCATGAAGCTGAGCCCTCAGGACC CGCGGCCCCTGTCCCCTGGGGCCTTGCAGCTTGCTGGAGAGAAGAGCAGTGAGAAGGGCC TGAGAGAGCGCGCCTACGGCAGCAGCGGGGAGCTCATCACCAGCCTGCCCATCAGCATCC CGCTCAGCACCGTGCAGCCCAACAAGCTCCCGGTCAGCATTCCCCTGGCCAGCGTGGTGC TGCCCAGCCGCGCCGAGAGGGCGAGGAGCACCCCCAGTCCCGTGCTGCAGCCCCGTGACC CCTCGTCCACACTTGAAAAGCAGATTGGTGCTAATGCCCACGGTGCTGGGAGCAGAAGCC TTGCCCTGGCCCCCGCAGGCTTCTCCTACGCTGGCTCGGTGGCCATCAGCGGGGCCTTGG CGGGCAGCCCGGCCTCTCTCACACCTGGAGCCGAGCCGGCCACCTTGGATGAGTCCTCCA GCTCTGGGAGCCTTTTTGCCACCGTGGGGTCCCGCAGCTCCACGCCACAGCACCCCCTGC TGCTGGCACAGCCCCGGAACTCGCTTCCTGCCTCTCCCGCCCACCAGCTCTCCTCCAGTC CCCGGCTTGGTGGGGCCGCCCAGGGCCCGTTGCCCGAGGCCAGCAAGGGAGACCTGCCCT CCGATTCCGGCTTCTCAGATCCTGAGAGTGAAGCCAAGAGGAGGATTGTGTTCACCATCA CCACTGGTGCGGGCAGTGCCAAGCAGTCGCCCTCCAGCAAGCACAGCCCCCTGACCGCCA GCGCCCGTGGGGACTGTGTGCCGAGCCACGGGCAGGACAGTCGCAGGCGCGGCCGGCGGA AGCGAGCATCTGCGGGGACGCCCAGCTTGAGCGCAGGCGTGTCCCCCAAGCGCCGAGCCC TGCCGTCCGTCGCTGGCCTTTTCACACAGCCTTCGGGGTCTCCCCTCAACCTCAACTCCA TGGTCAGTAACATCAACCAGCCCCTGGAGATTACAGCCATCTCGTCCCCGGAGACCTCCC TGAAGAGCTCCCCTGTGCCCTACCAGGACCACGACCAGCCCCCCGTGCTCAAGAAGGAGC GGCCTCTGAGCCAGACCAATGGGGCACACTACTCCCCACTCACCTCAGACGAGGAGCCAG GCTCTGAGGACGAGCCCAGCAGTGCTCGAATTGAGAGAAAAATTGCAACAATCTCCTTAG AAAGCAAATCTCCCCCGAAAACCTTGGAAAATGGTGGTGGCTTGGCGGGAAGGAAGCCCG CGCCCGCCGGCGAGCCAGTCAATAGCAGCAAGTGGAAGTCCACCTTCTCGCCCATCTCCG ACATCGGCCTGGCCAAGTCGGCGGACAGCCCGCTGCAGGCCAGCTCCGCCCTCAGCCAGA ACTCCCTGTTCACGTTCCGGCCCGCCCTGGAGGAGCCCTCTGCCGATGCCAAGCTGGCCG CTCACCCCAGGAAAGGCTTTCCCGGCTCCCTGTCGGGGGCTGACGGACTCAGCCCGGGCA CCAACCCTGCCAACGGCTGCACCTTCGGCGGGGGCCTGGCCGCGGACCTGAGTTTACACA GCTTCAGTGATGGTGCTTCTCTTCCCCACAAGGGCCCCGAGGCGGCCGGCCTGAGCTCCC CGCTGAGCTTCCCCTCGCAGCGCGGCAAGGAGGGCTCGGACGCCAACCCTTTCCTGAGCA AGAGGCAGCTGGACGGCCTGGCTGGGCTGAAGGGCGAGGGCAGCCGCGGCAAGGAGGCAG GGGAGGGCGGCCTACCGCTGTGCGGGCCCACGGACAAGACCCCACTGCTGAGCGGCAAGG CCGCCAAGGCCCGGGACCGCGAGGTCGACCTCAAGAATGGCCACAACCTCTTCATCTCTG CGGCGGCCGTGCCTCCCGGAAGCCTCCTCAGCGGCCCCGGCCTGGCCCCGGCGGCGTCCT CCGCAGGCGGCGCGGCGTCCTCCGCCCAGACGCACCGGTCCTTCCTGGGCCCCTTCCCGC CGGGACCGCAGTTCGCGCTCGGCCCCATGTCCCTGCAGGCCAACCTCGGCTCCGTGGCCG GCTCCTCCGTGCTGCAGTCGCTGTTCAGCTCTGTGCCGGCCGCCGCAGGCCTGGTGCACG TGTCGTCCGCTGCCACCAGACTGACCAACTCGCACGCCATGGGCAGCTTTTCCGGGGTGG CAGGCGGCACAGTTGGAGGTAACTAGGATTTCTACCTCAACCGCGAGACCTATGCAAGGA CGGTGTGGACCAACTCGCGCCCGCGGCATGGTGCCCGCCGGCCTGCCGGGCTCCCACCCC TGGACGGCAGAGGCAAGGACGGACGGGAGCTCCACTGTGAATCGGCGGCACGCGCCGCAG GAGGCTGGGACTGGTCCAGTTTGTACTGTCGATAGTTTTAGATAAAGTATTTATCATTTT TTAAAAAGTATAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_032482 |
| Insert Size | 5100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a good match to the protein associated to this reference except for a few SNPs. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032482.1, NP_115871.1 |
| RefSeq Size | 4889 bp |
| RefSeq ORF | 4614 bp |
| Locus ID | 84444 |
| UniProt ID | Q8TEK3 |
| Domains | AT_hook |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
| Gene Summary | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218547 | DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
CNY 15,776.00 |
|
| RC218547L1 | Lenti-ORF clone of DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
CNY 18,176.00 |
|
| RC218547L2 | Lenti-ORF clone of DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
CNY 13,970.00 |
|
| RC218547L3 | Lenti-ORF clone of DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
CNY 13,970.00 |
|
| RC218547L4 | Lenti-ORF clone of DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
CNY 18,176.00 |
|
| RG218547 | DOT1L (tGFP-tagged) - Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
CNY 17,376.00 |
