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LAMC2 (NM_018891) Human Untagged Clone
CAT＃: SC315189
LAMC2 (untagged)-Human laminin, gamma 2 (LAMC2), transcript variant 2
CNY 10,450.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_018891 edited
GTGAGAACCACCAACCGAGGCGCCGGGCAGCGACCCCTGCAGCGGAGACAGAGACTGAGC GGCCCGGCACCGCCATGCCTGCGCTCTGGCTGGGCTGCTGCCTCTGCTTCTCGCTCCTCC TGCCCGCAGCCCGGGCCACCTCCAGGAGGGAAGTCTGTGATTGCAATGGGAAGTCCAGGC AGTGTATCTTTGATCGGGAACTTCACAGACAAACTGGTAATGGATTCCGCTGCCTCAACT GCAATGACAACACTGATGGCATTCACTGCGAGAAGTGCAAGAATGGCTTTTACCGGCACA GAGAAAGGGACCGCTGTTTGCCCTGCAATTGTAACTCCAAAGGTTCTCTTAGTGCTCGAT GTGACAACTCTGGACGGTGCAGCTGTAAACCAGGTGTGACAGGAGCCAGATGCGACCGAT GTCTGCCAGGCTTCCACATGCTCACGGATGCGGGGTGCACCCAAGACCAGAGACTGCTAG ACTCCAAGTGTGACTGTGACCCAGCTGGCATCGCAGGGCCCTGTGACGCGGGCCGCTGTG TCTGCAAGCCAGCTGTTACTGGAGAACGCTGTGATAGGTGTCGATCAGGTTACTATAATC TGGATGGGGGGAACCCTGAGGGCTGTACCCAGTGTTTCTGCTATGGGCATTCAGCCAGCT GCCGCAGCTCTGCAGAATACAGTGTCCATAAGATCACCTCTACCTTTCATCAAGATGTTG ATGGCTGGAAGGCTGTCCAACGAAATGGGTCTCCTGCAAAGCTCCAATGGTCACAGCGCC ATCAAGATGTGTTTAGCTCAGCCCAACGACTAGACCCTGTCTATTTTGTGGCTCCTGCCA AATTTCTTGGGAATCAACAGGTGAGCTATGGTCAAAGCCTGTCCTTTGACTACCGTGTGG ACAGAGGAGGCAGACACCCATCTGCCCATGATGTGATTCTGGAAGGTGCTGGTCTACGGA TCACAGCTCCCTTGATGCCACTTGGCAAGACACTGCCTTGTGGGCTCACCAAGACTTACA CATTCAGGTTAAATGAGCATCCAAGCAATAATTGGAGCCCCCAGCTGAGTTACTTTGAGT ATCGAAGGTTACTGCGGAATCTCACAGCCCTCCGCATCCGAGCTACATATGGAGAATACA GTACTGGGTACATTGACAATGTGACCCTGATTTCAGCCCGCCCTGTCTCTGGAGCCCCAG CACCCTGGGTTGAACAGTGTATATGTCCTGTTGGGTACAAGGGGCAATTCTGCCAGGATT GTGCTTCTGGCTACAAGAGAGATTCAGCGAGACTGGGGCCTTTTGGCACCTGTATTCCTT GTAACTGTCAAGGGGGAGGGGCCTGTGATCCAGACACAGGAGATTGTTATTCAGGGGATG AGAATCCTGACATTGAGTGTGCTGACTGCCCAATTGGTTTCTACAACGATCCGCACGACC CCCGCAGCTGCAAGCCATGTCCCTGTCATAACGGGTTCAGCTGCTCAGTGATGCCGGAGA CGGAGGAGGTGGTGTGCAATAACTGCCCTCCCGGGGTCACCGGTGCCCGCTGTGAGCTCT GTGCTGATGGCTACTTTGGGGACCCCTTTGGTGAACATGGCCCAGTGAGGCCTTGTCAGC CCTGTCAATGCAACAACAATGTGGACCCCAGTGCCTCTGGGAATTGTGACCGGCTGACAG GCAGGTGTTTGAAGTGTATCCACAACACAGCCGGCATCTACTGCGACCAGTGCAAAGCAG GCTACTTCGGGGACCCATTGGCTCCCAACCCAGCAGACAAGTGTCGAGCTTGCAACTGTA ACCCCATGGGCTCAGAGCCTGTAGGATGTCGAAGTGATGGCACCTGTGTTTGCAAGCCAG GATTTGGTGGCCCCAACTGTGAGCATGGAGCATTCAGCTGTCCAGCTTGCTATAATCAAG TGAAGATTCAGATGGATCAGTTTATGCAGCAGCTTCAGAGAATGGAGGCCCTGATTTCAA AGGCTCAGGGTGGTGATGGAGTAGTACCTGATACAGAGCTGGAAGGCAGGATGCAGCAGG CTGAGCAGGCCCTTCAGGACATTCTGAGAGATGCCCAGATTTCAGAAGGTGCTAGCAGAT CCCTTGGTCTCCAGTTGGCCAAGGTGAGGAGCCAAGAGAACAGCTACCAGAGCCGCCTGG ATGACCTCAAGATGACTGTGGAAAGAGTTCGGGCTCTGGGAAGTCAGTACCAGAACCGAG TTCGGGATACTCACAGGCTCATCACTCAGATGCAGCTGAGCCTGGCAGAAAGTGAAGCTT CCTTGGGAAACACTAACATTCCTGCCTCAGACCACTACGTGGGGCCAAATGGCTTTAAAA GTCTGGCTCAGGAGGCCACAAGATTAGCAGAAAGCCACGTTGAGTCAGCCAGTAACATGG AGCAACTGACAAGGGAAACTGAGGACTATTCCAAACAAGCCCTCTCACTGGTGCGCAAGG CCCTGCATGAAGGAGTCGGAAGCGGAAGCGGTAGCCCGGACGGTGCTGTGGTGCAAGGGC TTGTGGAAAAATTGGAGAAAACCAAGTCCCTGGCCCAGCAGTTGACAAGGGAGGCCACTC AAGCGGAAATTGAAGCAGATAGGTCTTATCAGCACAGTCTCCGCCTCCTGGATTCAGTGT CTCGGCTTCAGGGAGTCAGTGATCAGTCCTTTCAGGTGGAAGAAGCAAAGAGGATCAAAC AAAAAGCGGATTCACTCTCAAGCCTGGTAACCAGGCATATGGATGAGTTCAAGCGTACAC AAAAGAATCTGGGAAACTGGAAAGAAGAAGCACAGCAGCTCTTACAGAATGGAAAAAGTG GGAGAGAGAAATCAGATCAGCTGCTTTCCCGTGCCAATCTTGCTAAAAGCAGAGCACAAG AAGCACTGAGTATGGGCAATGCCACTTTTTATGAAGTTGAGAGCATCCTTAAAAACCTCA GAGAGTTTGACCTGCAGGTGGACAACAGAAAAGCAGAAGCTGAAGAAGCCATGAAGAGAC TCTCCTACATCAGCCAGAAGGTTTCAGATGCCAGTGACAAGACCCAGCAAGCAGAAAGAG CCCTGGGGAGCGCTGCTGCTGATGCACAGAGGGCAAAGAATGGGGCCGGGGAGGCCCTGG AAATCTCCAGTGAGATTGAACAGGAGATTGGGAGTCTGAACTTGGAAGCCAATGTGACAG CAGATGGAGCCTTGGCCATGGAAAAGGGACTGGCCTCTCTGAAGAGTGAGATGAGGGAAG TGGAAGGAGAGCTGGAAAGGAAGGAGCTGGAGTTTGACACGAATATGGATGCAGTACAGA TGGTGATTACAGAAGCCCAGAAGGTTGATACCAGAGCCAAGAACGCTGGGGTTACAATCC AAGACACACTCAACACATTAGACGGCCTCCTGCATCTGATGGGTATGTGACTCAGTGTAG ATGAAGAGGGGCTGGTCTTACTGGAGCAGAAGCTTTCCCGAGCCAAGACCCAGATCAACA GCCAACTGCGGCCCATGATGTCAGAGCTGGAAGAGAGGGCACGTCAGCAGAGGGGCCACC TCCATTTGCTGGAGACAAGCATAGATGGGATTCTGGCTGATGTGAAGAACTTGGAGAACA TTAGGGACAACCTGCCCCAGGCTGCTAC |
| Restriction Sites | Please inquire |
| ACCN | NM_018891 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found the protein associated with this clone is identical to NM_018891.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018891.1, NP_061486.1 |
| RefSeq Size | 4297 bp |
| RefSeq ORF | 3336 bp |
| Locus ID | 3918 |
| UniProt ID | Q13753 |
| Domains | LamB, EGF_Lam |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
| Gene Summary | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) represents a shorter transcript variant, compared to variant 1, and encodes a shorter isoform (b). Transcript variant 2, unlike variant 1, has limited expression only in the embryonic cerebral cortex, lung and distal tubules of the kidney. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218516 | LAMC2 (Myc-DDK-tagged)-Human laminin, gamma 2 (LAMC2), transcript variant 2 |
CNY 7,736.00 |
|
| RC218516L1 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, Myc-DDK-tagged |
CNY 10,136.00 |
|
| RC218516L2 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RC218516L3 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC218516L4 | Lenti ORF clone of Human laminin, gamma 2 (LAMC2), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RG218516 | LAMC2 (tGFP-tagged) - Human laminin, gamma 2 (LAMC2), transcript variant 2 |
CNY 9,336.00 |
