ILF3 (NM_153464) Human Untagged Clone
CAT#: SC314442
ILF3 (untagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3
CNY 13,300.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF90; NF90a; NF90b; NF90c; NF90ctv; NF110; NF110b; NFAR; NFAR-1; NFAR-2; NFAR2; NFAR90; NFAR110; TCP80; TCP110 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC314442 representing NM_153464.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGTCCAATGCGAATTTTTGTGAATGATGACCGCCATGTGATGGCAAAGCATTCTTCCGTTTATCCA ACACAAGAGGAGCTGGAGGCAGTCCAGAACATGGTGTCCCACACGGAGCGGGCGCTCAAAGCTGTGTCC GACTGGATAGACGAGCAGGAAAAGGGTAGCAGCGAGCAGGCAGAGTCCGATAACATGGATGTGCCCCCA GAGGACGACAGTAAAGAAGGGGCTGGGGAACAGAAGACGGAGCACATGACCAGAACCCTGCGGGGAGTG ATGCGGGTGGGCCTGGTGGCAAAGGGCCTCCTACTCAAGGGGGACTTGGATCTGGAGCTGGTGCTGCTG TGTAAGGAGAAGCCCACAACCGCCCTCCTGGACAAGGTGGCCGACAACCTGGCCATCCAGCTTGCTGCT GTAACAGAAGACAAGTACGAAATACTGCAATCTGTCGACGATGCTGCGATTGTGATAAAAAACACAAAA GAGCCTCCATTGTCCCTGACCATCCACCTGACATCCCCTGTTGTCAGAGAAGAAATGGAGAAAGTATTA GCTGGAGAAACGCTATCAGTCAACGACCCCCCGGACGTTCTGGACAGGCAGAAATGCCTTGCTGCCTTG GCGTCCCTCCGACACGCCAAGTGGTTCCAGGCCAGAGCCAACGGGCTGAAGTCTTGTGTCATTGTGATC CGGGTCTTGAGGGACCTGTGCACTCGCGTGCCCACCTGGGGTCCCCTCCGAGGCTGGCCTCTCGAGCTC CTGTGTGAGAAATCCATTGGCACGGCCAACAGACCGATGGGTGCTGGCGAGGCCCTGCGGAGAGTGCTG GAGTGCCTGGCGTCGGGCATCGTGATGCCAGATGGTTCTGGCATTTATGACCCTTGTGAAAAAGAAGCC ACTGATGCTATTGGGCATCTAGACAGACAGCAACGGGAAGATATCACACAGAGTGCGCAGCACGCACTG CGGCTCGCTGCCTTCGGCCAGCTCCATAAAGTCCTAGGCATGGACCCTCTGCCTTCCAAGATGCCCAAG AAACCAAAGAATGAAAACCCAGTGGACTACACCGTTCAGATCCCACCAAGCACCACCTATGCCATTACG CCCATGAAACGCCCAATGGAGGAGGACGGGGAGGAGAAGTCGCCCAGCAAAAAGAAGAAGAAGATTCAG AAGAAAGAGGAGAAGGCAGAGCCCCCCCAGGCTATGAATGCCCTGATGCGGTTGAACCAGCTGAAGCCA GGGCTGCAGTACAAGCTGGTGTCCCAGACTGGGCCCGTCCATGCCCCCATCTTTACCATGTCTGTGGAG GTTGATGGCAATTCATTCGAGGCCTCTGGGCCCTCCAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAG GTGTTACAGGACATGGGCTTGCCGACGGGTGCTGAAGGCAGGGACTCGAGCAAGGGGGAGGACTCGGCT GAGGAGACCGAGGCGAAGCCAGCAGTGGTGGCCCCTGCCCCAGTGGTAGAAGCTGTCTCCACCCCTAGT GCGGCCTTTCCCTCAGATGCCACTGCCGAGCAGGGGCCGATCCTGACAAAGCACGGCAAGAACCCAGTC ATGGAGCTGAACGAGAAGAGGCGTGGGCTCAAGTACGAGCTCATCTCCGAGACCGGGGGCAGCCACGAC AAGCGCTTCGTCATGGAGGTCGAAGTGGATGGACAGAAGTTCCAAGGTGCTGGTTCCAACAAAAAGGTG GCGAAGGCCTACGCTGCTCTTGCTGCCCTAGAAAAGCTTTTCCCTGACACCCCTCTCGCCCTTGATGCC AACAAAAAGAAGAGAGCCCCAGTACCCGTCAGAGGGGGACCGAAATTTGCTGCTAAGCCACATAACCCT GGCTTCGGCATGGGAGGCCCCATGCACAACGAAGTGCCCCCACCCCCCAACCTTCGAGGGCGGGGAAGA GGCGGGAGCATCCGGGGACGAGGGCGCGGGCGAGGATTTGGTGGCGCCAACCATGGAGGCTACATGAAT GCCGGTGCTGGGTATGGAAGCTATGGGTACGGAGGCAACTCGGCGACAGCAGGCTACACAGGTTTTGTA TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_153464 |
Insert Size | 2073 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_153464.2 |
RefSeq Size | 3302 bp |
RefSeq ORF | 2073 bp |
Locus ID | 3609 |
UniProt ID | Q12906 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 74.6 kDa |
Gene Summary | This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014] Transcript Variant: This variant (3) differs in the 3' UTR and coding region compared to variant 4. The resulting isoform (c) has a distinct C-terminus compared to isoform d. This isoform is also called DRBP76 delta and ILF3-C. |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219617 | ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 |
CNY 5,056.00 |
|
RC219617L3 | Lenti-ORF clone of ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 |
CNY 9,120.00 |
|
RC219617L4 | Lenti-ORF clone of ILF3 (mGFP-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 |
CNY 9,120.00 |
|
RG219617 | ILF3 (tGFP-tagged) - Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 |
CNY 7,980.00 |