Tomosyn (STXBP5) (AK090549) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LGL3; LLGL3; Nbla04300
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090549, the custom clone sequence may differ by one or more nucleotides ATGCTGTGCATCGCTGGAGTTTCAGCTCATGTCATTATTTATAGATTCAGCAAGCAGGAA GTAATCACAGAAGTCATTCCGATGCTTGAAGTTCGATTATTATATGAGATAAATGATGTG GAAACTCCGGAGGGTGAGCAGCCACCACCTTTGCCAACACCCGTGGGAGGGTCCAACCCT CAGCCCATCCCTCCTCAGTCTCATCCATCTACCAGTAGCAGTTCATCTGATGGGCTTCGT GATAATGTACCTTGTTTAAAAGTTAAAAACTCACCACTTAAACAGTCTCCAGGTTATCAA ACAGAACTAGTTATTCAGTTGGTTTGGGTGGGTGGAGAACCACCACAACAAATAACCAGC CTGGCAGTCAATTCTTCCTATGGACTGGTGGTTTTTGGCAATTGCAATGGCATTGCTATG GTTGACTACCTCCAGAAAGCAGTGCTGCTCAACCTGGGCACTATTGAATTATATGGCTCT AATGATCCTTATCGGAGAGAACCCCGATCTCCTCGTAAATCTCGACAGCCTTCAGGAGCC GGTCTGTGTGATATTAGTGAAGGGACTGTTGTTCCAGAGGATCGCTGCAAATCTCCAACC TCTGGTTCTTCATCACCACACAATTCAGATGATGAACAAAAAATGAATAATTTTATAGAA AAGGTGAAGACCAAAAGCAGAAAGTTTTCCAAGATGGTAGCCAATGATATAGCAAAGATG TCAAGGAAGTTAAGCTTACCTACTGACCTAAAGCCTGATTTAGATGTAAAGGATAACTCC TTTAGCCGATCACGGAGTTCAAGTGTAACAAGCATTGACAAAGAATCCCGAGAAGCGATC TCCGCTCTTCATTTCTGTGAAACGTTTACTCGAAAGACGGACTCGTCCCCTTCCCCTTGT CTGTGGGTTGGAACAACGCTAGGAACAGTGCTTGTCATTGCACTGAACCTTCCCCCAGGG GGAGAGCAAAGACTTCTTCAGCCAGTAATTGTGTCTCCAAGTGGTACTATATTGAGGTTA AAAGGTGCAATCTTGAGAATGGCATTTCTGGATACCACAGGCTGCTTAATACCACCTGCG TATGAACCCTGGAGAGAGCACAATGTTCCTGAAGAAAAAGACGAAAAGGAGAAATTGAAA AAACGGCGGCCTGTCTCAGTATCCCCCTCCTCTTCTCAGGAAATTAGTGAAAACCAGTAT GCAGTGATATGTTCTGAAAAGCAAGCAAAAGTAATCTCACTGCCAACCCAGAACTGTGCT TATAAGCAAAATATTACAGAGACCTCGTTTGTGCTTCGTGGAGATATTGTAGCATTGAGT AACAGTATCTGCCTTGCCTGTTTCTGTGCCAATGGACATATAATGACTTTTAGTTTGCCA AGTTTAAGACCTCTGTTGGATGTGTATTACTTGCCCCTTACCAATATGCGGATAGCCAGA ACGTTCTGCTTTACCAACAATGGACAAGCATTATACCTTGTTTCACCTACAGAAATCCAG AGACTTACTTATAGTCAAGAGACCTGTGAAAATCTTCAGGAAATGTTGGGTGAACTCTTC ACTCCTGTAGAAACACCTGAAGCACCAAACAGGGGATTCTTTAAAGGCTTATTTGGAGGT GGTGCACAATCTCTTGACAGAGAAGAACTATTTGGAGAATCGTCCTCAGGAAAGGCTTCA AGGAGCCTTGCACAGCATATTCCTGGCCCTGGTGGCATTGAAGGCGTAAAAGGGGCAGCA TCTGGAGTTGTTGGTGAATTAGCACGAGCCAGGCTGGCACTAGATGAAAGAGGGCAGAAA CTTGGCGATCTGGAAGAAAGAACTGCGGCCATGTTATCAAGTGCAGAGTCATTTTCTAAA CATGCTCATGAGATTATGTTGAAATACAAAGATAAGAAGTGGTACCAGTTC
Restriction Sites	Please inquire
ACCN	AK090549
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK090549.1, BAC03475.1
RefSeq Size	3316 bp
RefSeq ORF	1914 bp
Locus ID	134957
Gene Summary	Syntaxin 1 is a component of the 7S and 20S SNARE complexes which are involved in docking and fusion of synaptic vesicles with the presynaptic plasma membrane. This gene encodes a syntaxin 1 binding protein. In rat, a similar protein dissociates syntaxin 1 from the Munc18/n-Sec1/rbSec1 complex to form a 10S complex, an intermediate which can be converted to the 7S SNARE complex. Thus this protein is thought to be involved in neurotransmitter release by stimulating SNARE complex formation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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