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GIT2 (NM_057170) Human Untagged Clone
CAT＃: SC314271
GIT2 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2
CNY 10,170.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAT-2; CAT2; PKL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314271 representing NM_057170.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCA GTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGCCATATCTCC CAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAAT AACGGTGCTAACTCTATATGGGAGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAA GCTAATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCG TTCGTCCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCAT TCGAGCGTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAACTTC TTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATTTTACAGGCTGAA TTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCA AGGCAAGGAGGGCACCATGAGCTGGCAGAGCGCCTCGTGGAAATACAGTATGAGCTAACGGACAGACTA GCCTTCTATCTCTGTGGCAGGAAACCAGATCACAAAAATGGACAGCACTTTATAATACCTCAAATGGCA GACAGCAGCCTGGATTTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCAT TTGTTTGAAGAACTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGGCTT GCCACGCAAAACCACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCGGTCAATCCTGAG TACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAACGCCCATGAGTTTGCCACGCTG GTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAGGGCAGTTCTCTCTCGGGTTCAAAAGACAAT GTGGAGCTCATACTGAAAACCATCAATAACCAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGAC TATGACAGCGTGGCATCAGACGAAGACACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAG AGCCTAGATTCAGATTTATCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTA GTGGCTTCTGAGGCCAAGATACAGCAGCTAATGAAGGCATCCAGGCTGGAGAAGCAGAACAGCACACCT GAGAGTGACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGGACGG CAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTACCAGACACAGCAGAACCCCATGTGGCCCCAAGC CCCACTCTCCCTAGCACCGAAGATGTCATCAGGAAGACTGAACAGATCACCAAAAACATACAGGAGCTC TTAAGAGCAGCCCAAGAAAATAAACATGACAGTTATATTCCCTGCTCAGAGAGGATACACGTAGCTGTT ACAGAAATGGCAGCATTATTCCCCAAAAAACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTG ACGTCCAGTGCCTACCGACTGCAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACA GACGTTCAGCTGGTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTG GTTACCATCACCACCAAAGAGAACAACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_057170 |
| Insert Size | 1896 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_057170.3 |
| RefSeq Size | 5259 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 9815 |
| UniProt ID | Q14161 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis |
| MW | 70.2 kDa |
| Gene Summary | This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (2) lacks two exons and a portion of a third exon in the coding region, as compared to variant 1. The reading frame is maintained. Isoform 2 is missing internal 128 aa but has the same N- and C-termini as isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219972 | GIT2 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2 |
CNY 4,696.00 |
|
| RC219972L3 | Lenti-ORF clone of GIT2 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2 |
CNY 5,890.00 |
|
| RC219972L4 | Lenti-ORF clone of GIT2 (mGFP-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2 |
CNY 5,890.00 |
|
| RG219972 | GIT2 (tGFP-tagged) - Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2 |
CNY 4,370.00 |
