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PPEF1 (NM_152224) Human Untagged Clone
CAT＃: SC314250
PPEF1 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1b
CNY 10,070.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PP7; PPEF; PPP7C; PPP7CA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314250 representing NM_152224.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGATGCAGCAGTTCTTCAACGAAAACCAGGAGATCTGACACATCACTGAGAGCTGCGTTGATCATC CAGAACTGGTACCGAGGTTACAAAGCTCGACTGAAGGCCAGACAACACTATGCCCTCACCATCTTCCAG TCCATCGAATATGCTGATGAACAAGGCCAAATGCAGTTATCCACCTTCTTTTCCTTCATGTTGGAAAAC TACACACATATACATAAGGAAGAGCTAGAATTAAGAAATCAGTCTCTTGAAAGCGAACAGGACATGAGG GATAGATGGGATTATGTGGACTCGATAGATGTCCCAGACTCCTATAATGGTCCTCGGCTACAATTTCCT CTCACTTGTACGGATATTGATTTACTTCTTGAGGCCTTCAAGGAACAACAGATACTTCATGCCCATTAT GTCTTAGAGGTGCTATTTGAAACCAAGAAAGTCCTGAAGCAAATGCCGAATTTCACTCACATACAAACT TCTCCCTCCAAAGAGGTAACAATCTGTGGTGATTTGCATGGGAAACTGGATGATCTTTTTTTGATCTTC TACAAGAATGGTCTCCCCTCAGAGAGGAACCCGTATGTTTTTAATGGTGACTTTGTAGATCGAGGAAAG AATTCCATAGAGATCCTAATGATCCTGTGTGTGAGTTTTCTTGTCTACCCCAATGACCTGCACTTGAAC AGAGGGAACCACGAAGATTTTATGATGAATCTGAGGTATGGCTTCACGAAAGAAATTTTGCATAAATAT AAGCTACATGGAAAAAGAATCTTACAAATCTTGGAAGAATTCTATGCCTGGCTCCCAATCGGTACAATC GTTGACAATGAAATCCTGGTCATCCATGGTGGGATATCAGAGACCACAGACTTGAATTTACTCCACCGT GTAGAGAGGAACAAGATGAAATCTGTGCTGATACCACCAACGGAAACAAACAGAGACCATGACACTGAC TCGAAGCACAATAAAATTATTGATATTCTGTGGAGTGATCCCAGAGGCAAAAATGGCTGTTTTCCAAAT ACGTGCCGAGGAGGGGGCTGCTATTTTGGACCAGATGTTACTTCCAAGATTCTTAATAAATACCAGTTG AAGATGCTCATCAGGTCTCATGAATGTAAGCCCGAAGGGTATGAAATCTGTCATGATGGGAAGGTGGTG ACTATATTTTCTGCTTCTAATTATTATGAAGAAGGCAGCAATCGAGGAGCTTACATCAAACTATGTTCT GGTACAACTCCTCGATTTTTCCAGTACCAAGTAACTAAAGCAACGTGCTTTCAGCCTCTTCGCCAAAGA GTGGATACTATGGAAAACAGCGCCATCAAGATATTAAGAGAGAGAGTGATTTCACGAAAAAGTGACCTT ACTCGTGCTTTCCAACTTCAAGACCACAGAAAATCAGGAAAACTTTCTGTGAGCCAGTGGGCTTTTTGC ATGGAGAACATTTTGGGGCTGAACTTACCATGGAGATCCCTCAGTTCGAATCTGGTAAACATAGACCAA AATGGAAACGTTGAATACATGTCCAGCTTCCAGAATATCCGCATTGAAAAACCTGTACAAGAGGCTCAT TCTACTCTAGTTGAAACTCTGTACAGATACAGATCTGACCTGGAAATCATATTTAATGCCATTGACACT GATCACTCAGGCCTGATCTCCGTGGAAGAATTTCGTGCCATGTGGAAACTTTTTAGTTCTCACTACAAT GTTCACATTGATGATTCCCAAGTCAATAAGCTTGCCAACATAATGGACTTGAACAAAGATGGAAGCATT GACTTTAATGAGTTTTTAAAGGCTTTCTATGTAGTGCATAGATATGAAGACTTGATGAAACCTGATGTC ACCAACCTTGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_152224 |
| Insert Size | 1878 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152224.1 |
| RefSeq Size | 2789 bp |
| RefSeq ORF | 1878 bp |
| Locus ID | 5475 |
| UniProt ID | O14829 |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 72.6 kDa |
| Gene Summary | This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1b) uses an alternate in-frame splice site compared to variant 1, resulting in an isoform (1b) that lacks an internal segment compared to isoform 1. Isoform 1b contains a catalytic domain and two EF-hand motifs. Its functional significance is unknown. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213104 | PPEF1 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1b |
CNY 4,656.00 |
|
| RC213104L3 | Lenti-ORF clone of PPEF1 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1b |
CNY 5,890.00 |
|
| RC213104L4 | Lenti-ORF clone of PPEF1 (mGFP-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1b |
CNY 5,890.00 |
|
| RG213104 | PPEF1 (tGFP-tagged) - Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 1b |
CNY 4,370.00 |
