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KLHL18 (NM_025010) Human Untagged Clone
CAT＃: SC314064
KLHL18 (untagged)-Human kelch-like 18 (Drosophila) (KLHL18)
CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314064 representing NM_025010.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGGAGGACGGCGCGGAGGAGCTGGAGGATCTGGTGCACTTCTCCGTGTCTGAGTTGCCTAGTCGC GGCTACGGCGTCATGGAGGAGATCCGGCGGCAGGGCAAGCTGTGCGACGTGACCCTCAAGATTGGGGAC CACAAATTCAGTGCCCACCGGATTGTCTTAGCAGCCTCGATCCCGTATTTCCATGCTATGTTTACAAAT GACATGATGGAGTGCAAGCAGGATGAGATTGTAATGCAAGGAATGGACCCAAGTGCCCTGGAGGCTCTG ATCAACTTTGCCTACAACGGCAACCTTGCCATTGACCAGCAAAATGTCCAGTCATTGCTGATGGGGGCG AGCTTCCTGCAGCTGCAGAGCATCAAAGACGCCTGCTGCACATTCCTTCGAGAACGGCTTCACCCAAAA AACTGCCTGGGTGTGCGCCAGTTTGCTGAGACAATGATGTGTGCTGTGCTGTACGACGCTGCCAACAGC TTCATCCACCAGCACTTTGTGGAGGTGTCCATGTCAGAAGAGTTCCTGGCCCTGCCCTTGGAAGACGTG CTTGAGCTGGTGTCTCGGGATGAGCTGAATGTCAAATCTGAGGAGCAGGTCTTTGAAGCTGCATTGGCC TGGGTCAGATACGACCGGGAGCAGAGGGGTCCCTACCTGCCTGAGCTGCTGTCCAATATCCGCCTGCCC CTCTGTCGGCCCCAGTTCCTTTCAGACAGAGTACAGCAGGATGACCTGGTGCGTTGCTGCCACAAATGC AGGGACCTGGTAGACGAAGCAAAGGACTACCACCTCATGCCAGAGCGCCGGCCCCACCTGCCAGCTTTC AGAACCCGGCCACGCTGCTGCACATCCATCGCTGGACTTATCTACGCTGTAGGGGGCCTCAACTCAGCA GGTGATTCCCTGAATGTGGTGGAAGTGTTCGACCCCATTGCCAATTGCTGGGAGAGATGCCGTCCCATG ACAACAGCCCGCAGCCGCGTTGGCGTGGCTGTGGTGAACGGGCTTCTCTATGCCATCGGAGGATATGAC GGCCAGCTACGGCTGAGCACTGTGGAGGCCTACAACCCGGAGACAGACACATGGACCAGAGTGGGGAGC ATGAATAGCAAGAGAAGTGCCATGGGGACAGTCGTGCTGGATGGGCAGATCTACGTCTGTGGGGGCTAC GATGGCAACTCTTCCCTCAGCTCCGTGGAGACCTACTCACCTGAGACGGACAAATGGACAGTGGTGACC TCGATGAGCTCGAATCGCAGTGCTGCTGGGGTTACAGTCTTTGAGGGCAGGATATATGTGTCAGGCGGC CATGATGGTTTGCAGATCTTCAGCAGTGTGGAACACTACAACCACCACACAGCCACCTGGCACCCTGCA GCTGGCATGCTCAACAAGCGCTGCCGGCACGGAGCCGCCTCCCTGGGGAGCAAGATGTTTGTCTGCGGG GGCTACGATGGCTCTGGCTTCCTCAGCATTGCCGAGATGTACAGCTCTGTGGCAGACCAGTGGTGCCTG ATTGTCCCCATGCACACGCGCAGGAGCCGGGTCTCCCTGGTGGCCAGCTGTGGGCGCCTCTACGCTGTT GGGGGCTACGACGGACAGTCAAACCTAAGCTCAGTGGAGATGTATGACCCAGAGACAGACTGCTGGACA TTCATGGCCCCCATGGCGTGCCATGAGGGAGGGGTCGGTGTGGGCTGCATCCCTCTCCTCACCATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025010 |
| Insert Size | 1725 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025010.4 |
| RefSeq Size | 4743 bp |
| RefSeq ORF | 1725 bp |
| Locus ID | 23276 |
| UniProt ID | O94889 |
| Domains | Kelch |
| MW | 63.6 kDa |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis (PubMed:23213400). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (PubMed:23213400). Regulates light-and dark-dependent alpha-transducin localization changes in rod photoreceptors through UNC119 ubiquitination and degradation (By similarity). Preferentially ubiquitinates the unphosphorylated form of UNC119 over the phosphorylated form (By similarity). In the presence of UNC119, under dark-adapted conditions alpha-transducin mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223003 | KLHL18 (Myc-DDK-tagged)-Human kelch-like 18 (Drosophila) (KLHL18) |
CNY 4,272.00 |
|
| RC223003L3 | Lenti ORF clone of Human kelch-like 18 (Drosophila) (KLHL18), Myc-DDK-tagged |
CNY 6,940.00 |
|
| RC223003L4 | Lenti ORF clone of Human kelch-like 18 (Drosophila) (KLHL18), mGFP tagged |
CNY 6,940.00 |
|
| RG223003 | KLHL18 (tGFP-tagged) - Human kelch-like 18 (Drosophila) (KLHL18) |
CNY 5,510.00 |
