PIGW (NM_178517) Human Untagged Clone
CAT#: SC313764
PIGW (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW)
CNY 6,016.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | Gwt1; HPMRS5 |
Vector | pCMV6 series |
Restriction Sites | Please inquire |
ACCN | NM_178517 |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_178517.2, NP_848612.1 |
RefSeq Size | 2255 bp |
RefSeq ORF | 1515 bp |
Locus ID | 284098 |
UniProt ID | Q7Z7B1 |
Protein Families | Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
Gene Summary | The protein encoded by this gene is an inositol acyltransferase that acylates the inositol ring of phosphatidylinositol. This occurs in the endoplasmic reticulum and is a step in the biosynthesis of glycosylphosphatidylinositol (GPI), which anchors many cell surface proteins to the membrane. Defects in this gene are a cause of the age-dependent epileptic encephalopathy West syndrome as well as a syndrome exhibiting hyperphosphatasia and cognitive disability (HPMRS5). [provided by RefSeq, Jul 2017] Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217028 | PIGW (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 4,470.00 |
|
RC217028L3 | Lenti-ORF clone of PIGW (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 6,370.00 |
|
RC217028L4 | Lenti-ORF clone of PIGW (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 6,370.00 |
|
RG217028 | PIGW (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 4,940.00 |