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MARCH I (MARCH1) (NM_017923) Human Untagged Clone
CAT＃: SC312747
41334 (untagged)-Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MARCH-I; MARCH1; RNF171 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC312747 representing NM_017923.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCAGCAGCCACGTTTGTTGTAATTTTTTAAATATGTGGAAAAAAAGCAAGATATCAACCATGTAT TACCTTAACCAAGATGCCAAATTATCTAACTTGTTTCTCCAGGCAAGCAGCCCAACAACAGGGACAGCT CCCAGGAGCCAGTCAAGGTTGTCTGTCTGTCCATCCACTCAGGACATCTGCAGAATCTGTCACTGCGAA GGGGATGAAGAGAGCCCCCTCATCACACCCTGTCGCTGCACTGGGACACTGCGCTTTGTCCACCAGTCC TGCCTCCACCAGTGGATAAAGAGCTCAGATACACGCTGCTGTGAGCTCTGCAAGTATGACTTCATAATG GAGACCAAGCTCAAACCCCTCCGGAAGTGGGAGAAACTACAGATGACCACAAGTGAAAGGAGGAAAATA TTCTGCTCTGTCACATTCCACGTAATCGCGATCACCTGTGTGGTTTGGTCTTTGTATGTATTGATAGAC CGGACAGCGGAGGAAATCAAGCAAGGCAATGACAATGGTGTCCTTGAATGGCCATTTTGGACAAAACTG GTTGTGGTAGCCATTGGCTTCACAGGAGGTCTTGTCTTCATGTACGTACAGTGTAAAGTCTATGTTCAG TTGTGGCGCAGGCTGAAGGCCTACAACCGTGTGATCTTTGTACAAAATTGCCCAGACACTGCCAAAAAA CTGGAGAAGAACTTCTCATGTAATGTAAACACAGACATCAAAGATGCTGTGGTAGTGCCTGTACCACAA ACAGGTGCAAATTCACTGCCATCTGCAGAGGGTGGCCCCCCTGAAGTTGTATCAGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017923 |
| Insert Size | 819 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017923.3 |
| RefSeq Size | 5389 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 55016 |
| UniProt ID | Q8TCQ1 |
| Domains | RING |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 30.9 kDa |
| Gene Summary | MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (2) lacks multiple exons at its 5' end and has a novel segment at its 5' end, compared to variant 1. These differences result in a protein (isoform 2) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222000 | MARCH1 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2 |
CNY 2,400.00 |
|
| RC222000L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222000L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG222000 | 41334 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2 |
CNY 4,370.00 |
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